Reaction of somaclonal lines of winter triticale to Fusarium infection
Part I. Fusarium head blight caused by F. culmorum W. G. Smith (Sacc.)
Tomasz Góral
t.goral@ihar.edu.plZakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Edward Arseniuk
Zakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
Resistance to Fusarium head blight of somaclonal lines (generations R1–R3) generated from 20 parental genotypes was studied. Heads were artificially inoculated with Fusarium culmorum isolates. Significant variation among parental genotypes in the resistance and tolerance to Fusarium head blight was found. Somaclonal lines generated from different parental genotypes (of different resistance) and the lines generated from different calli of the same parental genotype varied significantly in the level of resistance. Mean levels of resistance and tolerance for consecutive generations of lines did not differ significantly, which proved the stability of reaction of the lines to head blight. The increase or decrease of resistance to the level determined for parental genotype was observed only with some lines. The results presented in the paper show that the use of somaclonal variation can result in the stable improvement of the genotype resistance.
Keywords:
Fusarium culmorum, head blight, in vitro, resistance, somaclonal line, triticaleReferences
Adkins S. W., Kunanuvatchaidach R., Godwin I. D. 1995, Somaclonal variation in rice — drought tolerance and other agronomic characters. Aust. J. Bot. 43: 201 — 209.
DOI: https://doi.org/10.1071/BT9950201
Google Scholar
Ahmed, K. Z., A. Mesterhazy, T. Bartok, Sagi F. 1996. In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. II. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones. Euphytica 91: 341 — 349.
DOI: https://doi.org/10.1007/BF00033096
Google Scholar
Arseniuk E., Czembor H. J., Zimny J., Scharen A. L., Laudański Z. 1998. Somaclonal variation as a tool for improvement of triticale resistance to Stagonospora nodorum. Proc. 4th International Triticale Symp., July 26-31, 1998, Red Deer, Canada: 124 — 147.
Google Scholar
Breiman A., Rotem-Abarbanell D., Karp A., Shaskin H. 1987. Heritable somaclonal variation in wild barley (Hordeum sponatneum). Theor. Appl. Genet. 74: 104 — 112.
DOI: https://doi.org/10.1007/BF00290092
Google Scholar
Brettell R. I. S., Pallotta M. A., Gustafson J. P., Appels R. 1986. Variation at the Nor loci in triticale derived from tissue culture. Theor. Appl. Genet. 71: 637 — 643.
DOI: https://doi.org/10.1007/BF00264268
Google Scholar
Bretziger P., Halbert S. E., Lemaux P. G. 1998. Somaclonal variation in the progeny of transgenic barley. Theor. Appl. Genet 96: 421 — 425.
DOI: https://doi.org/10.1007/s001220050758
Google Scholar
Bruins M. B. M., Karsai I., Schepers J., Snijders C. H. A. 1993. Phytotoxicity of deoxynivalenol to wheat tissue with regard to in vitro selection for Fusarium head blight resistance. Plant Sci. 94: 195 — 206.
DOI: https://doi.org/10.1016/0168-9452(93)90020-Z
Google Scholar
Burgess L. W., Summerell B. A., Bullock S., Gott K. P., Backhouse D. 1994. Laboratory manual for Fusarium research. Third ed. University of Sydney, Australia.
Google Scholar
Chowdhury M. K. U., Vasil I. K. 1993. Molecular analysis of plants regenerated from embryogenic cultures of hybrid sugarcane cultivars (Saccharum spp.). 86: 181 — 188.
DOI: https://doi.org/10.1007/BF00222077
Google Scholar
Chowdhury M. K. U., Vasil V., Vasil I. K. 1994. Molecular analysis of plants regenerated from embryogenic cultures of wheat (Triticum aestivum L.). Theor. Appl. Genet. 87: 821 — 828.
DOI: https://doi.org/10.1007/BF00221134
Google Scholar
Góral T., Foremska E., Chełkowski J., Arseniuk E. 1995. Charakterystyka odmian pszenżyta, pszenicy i żyta pod względem odporności i tolerancji na porażenie kłosa przez Fusarium spp. Biuletyn IHAR 195/196: 251 — 259.
Google Scholar
Góral T., Perkowski J., Stachowiak J., Arseniuk E. 2000. Podatność na fuzariozę kłosów (Fusarium culmorum) oraz akumulacja deoksyniwalenolu w ziarnie linii somaklonalnych uzyskanych z odmian pszenżyta ozimego Moniko i Presto. Fol. Univ. Agric. Stetin. 82: 73 — 78.
Google Scholar
Góral T., Buśko M., Cichy H., Jackowiak H., Perkowski J. 2002. Resistance of winter triticale lines and cultivars to Fusarium head blight and deoxynivalenol accumulation in kernels. J. Appl. Genet 43A: 237 — 248
Google Scholar
Góral T., Perkowski J., Arseniuk E. 2002. Study on Fusarium head blight of winter triticale. Proc. 5th International Triticale Symposium, June 30–July 5, 2002, Radzików, Poland, Vol. I: 179 — 184.
Google Scholar
Hessemann C. U., Maier F. J. 1998. Application of biotechnological methods as tissue and suspension culture techniques in triticale. Proc. 4th International Triticale Symp., July 26-31, 1998, Red Deer, Canada: 30 — 37.
Google Scholar
Ivanov P., Atanassov Z., Milkova V., Nikolova L. 1998. Culture selected somaclonal variation in five Triticum aestivum L. genotypes. Euphytica 104: 167 — 172.
DOI: https://doi.org/10.1023/A:1018656903559
Google Scholar
Karp A. 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85: 295 — 302.
DOI: https://doi.org/10.1007/BF00023959
Google Scholar
Lapitan N. L. V., Sears R. G., Gill B. S. 1984. Translocations and other karyotypic structural changes in wheat × rye hybrids regenerated from tissue culture. Theor. Appl. Genet. 68: 547 — 554.
DOI: https://doi.org/10.1007/BF00285012
Google Scholar
Larkin P. J., Ryan S. A., Brettell R. I. S., Scowcroft W. R. 1984. Heritable somaclonal variation in wheat. Theor. Appl. Genet. 67: 443 — 455.
DOI: https://doi.org/10.1007/BF00263410
Google Scholar
Liang G. H., McHughen A. 1987. Novel approaches to wheat improvement. Wheat and wheat improvement. Agronomy Monograph 13: 472 — 488.
DOI: https://doi.org/10.2134/agronmonogr13.2ed.c28
Google Scholar
Maier F. J., Oettler G. 1996. Genetic variation for head blight resistance in triticale caused by Fusarium graminearum isolates of different deoxynivalenol production. Euphytica 89: 387 — 394.
DOI: https://doi.org/10.1007/BF00022297
Google Scholar
Miedaner T. 1997. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding 116: 201 — 220.
DOI: https://doi.org/10.1111/j.1439-0523.1997.tb00985.x
Google Scholar
Miedaner T., Reinbrecht C., Lauber U., Schollenberger M., Geiger H. 2001. Effects of genotype and genotype-environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breeding 120: 97 — 105.
DOI: https://doi.org/10.1046/j.1439-0523.2001.00580.x
Google Scholar
Parry D. W., Jenkinson P., McLeod L. 1995. Fusarium ear scab in small grain cereals — a review. Plant Pathol. 44: 207 — 238.
DOI: https://doi.org/10.1111/j.1365-3059.1995.tb02773.x
Google Scholar
Perkowski J. 1999. Badania zawartości toksyn fuzaryjnych w ziarnie zbóż. Rocz. AR w Poznaniu, Rozprawy Naukowe, Zeszyt 295.
Google Scholar
Posslet U. K., Altpeter F. 1994. Improvement of snow mould resistance by conventional and in vitro techniques. Euphytica 77: 251 — 255.
DOI: https://doi.org/10.1007/BF02262638
Google Scholar
Semal J., Kummert P., Lepoivre P., Meulemans, Viseur J., Anceau C. 1988. In vitro cultures producing pathogen-free plants and selecting disease resistant genotypes. Bull. Rech. Agron. Gembloux 23: 261 — 269.
Google Scholar
Symillides Y., Henry Y., De Buyser J. 1995. Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis. Euphytica 82: 263 — 268.
DOI: https://doi.org/10.1007/BF00029569
Google Scholar
Van den Bulk R.W. 1991. Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review. Euphytica 56: 269 — 285.
DOI: https://doi.org/10.1007/BF00042373
Google Scholar
Yang Z. P., Yang X. Y., Huang D. C. 1998. Studies on somaclonal variants for resistance to scab in bread wheat (Triticum aestivum L.) through in vitro selection for tolerance to deoxynivalenol. Euphytica 101: 213 — 219.
DOI: https://doi.org/10.1023/A:1018354606939
Google Scholar
Authors
Tomasz Góralt.goral@ihar.edu.pl
Zakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Edward ArseniukZakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Statistics
Abstract views: 182PDF downloads: 26
License
Copyright (c) 2003 Tomasz Góral, Edward Arseniuk

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Tomasz Góral, Halina Wiśniewska, Piotr Ochodzki, Dorota Walentyn-Góral, Iga Grzeszczak, Jolanta Belter, Maciej Majka, Jerzy Bogacki, Tadeusz Drzazga, Bogusława Ługowska, Przemysław Matysik, Edward Witkowski, Krzysztof Rubrycki, Urszula Woźna-Pawlak, Fuzarioza kłosów oraz akumulacja toksyn fuzaryjnych w ziarnie rodów hodowlanych pszenicy ozimej , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Maciej Majka, Magdalena Gawłowska, Adriana Twardawska, Marek Korbas, Jakub Danielewicz, Tomasz Góral, Bogusława Ługowska, Jolanta Belter, Edward Witkowski, Tadeusz Drzazga, Przemysław Matysik, Urszula Woźna-Pawlak, Halina Wiśniewska, Use of molecular and phenotypic markers to identify wheat eyespot resistance genes caused by Oculimacula yallundae and O. acuformis , Bulletin of Plant Breeding and Acclimatization Institute: No. 288 (2020): Regular issue
- Tomasz Góral, Dorota Walentyn-Góral, Variation for resistance to Fusarium head blight in winter and spring wheat varieties studied in 2009–2016. , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Tomasz Góral, Piotr Ochodzki, Zofia Bulińska-Radomska, Resistance to Fusarium head blight caused by Fusarium culmorum and Fusarium mycotoxins content in grain of spring cereal species intended for organic farming , Bulletin of Plant Breeding and Acclimatization Institute: No. 263 (2012): Regular issue
- Wiesław Golka, Edward Arseniuk, Adrian Golka, Tomasz Góral, Artificial neural networks and remote sensing in the assessment of spring wheat infection by Fusarium head blight , Bulletin of Plant Breeding and Acclimatization Institute: No. 288 (2020): Regular issue
- Tomasz Góral, Piotr Ochodzki, Linda Kærgaard Nielsen , Dorota Walentyn-Góral, Species of the genus Fusarium and Fusarium toxins in the grain of winter and spring wheat in Poland , Bulletin of Plant Breeding and Acclimatization Institute: No. 296 (2021): Regular issue
- Tomasz Góral, Piotr Ochodzki, Linda Kærgaard Nielsen, Dorota Walentyn-Góral, Fusarium species and Fusarium mycotoxins in grain of barley in Poland in 2009 and 2010. Short communication , Bulletin of Plant Breeding and Acclimatization Institute: No. 288 (2020): Regular issue
- Elżbieta Małuszyńska, Tomasz Góral, Dorota Walentyn-Góral, Effect of pre-chilling on germination of infected by Fusarium spp. winter wheat kernels. , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Tomasz Góral, Fusarium head blight of wheat. Part 1. Disease description and characteristics of pathogens , Bulletin of Plant Breeding and Acclimatization Institute: No. 300 (2023): Regular issue
- Tomasz Góral, Dorota Walentyn-Góral, Halina Wiśniewska, Fusarium head blight resistance of types I and II in wheat and triticale , Bulletin of Plant Breeding and Acclimatization Institute: No. 277 (2015): Regular issue








