Application of the Taguchi method in the androgenesis efficiency enhancment in cereal tissue cultures
Renata Orłowska
r.orlowska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0001-7150-6920
Katarzyna Pachota
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
Joanna Machczyńska
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0003-3383-8443
Agnieszka Niedziela
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0002-2886-8835
Janusz Zimny
Zakład Biotechnologii i Cytogenetyki Roślin (Poland)
https://orcid.org/0000-0002-1521-0874
Piotr T. Bednarek
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0002-1553-8378
Abstract
In cereal tissue cultures, one of the most difficult problems to overcome during the production of doubled haploids (DH) is the albinism. It seems that a quite effective way to reduce albinism is to choose the right stress factor in combination with a balanced composition of medium. In the presented work supplementation of nutrients with copper and silver ions (CuSO4, AgNO3) and modulation of cultures time on induction medium for three cereal species: barley (Hordeum vulgare L.), wheat (Triticum aestivum L.) and triticale (x Triticosecale spp. Wittmack ex A. Camus 1927) were used. Application of the Taguchi method enabled to limit the number of experiments and to optimize the conditions for tissue cultures, which enhanced of the amount of green regenerants in all analyzed species.
Keywords:
Taguchi method, optimization, cereal tissue cultures, CuSO4, AgNO3, induction lengthOrłowska, R. (2019) “Application of the Taguchi method in the androgenesis efficiency enhancment in cereal tissue cultures”, Bulletin of Plant Breeding and Acclimatization Institute, (287), pp. 29–30. doi: 10.37317/biul-2019-0102.
Authors
Renata Orłowskar.orlowska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0001-7150-6920
Authors
Katarzyna PachotaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
Authors
Joanna MachczyńskaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0003-3383-8443
Authors
Agnieszka NiedzielaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0002-2886-8835
Authors
Janusz ZimnyZakład Biotechnologii i Cytogenetyki Roślin Poland
https://orcid.org/0000-0002-1521-0874
Authors
Piotr T. BednarekInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0002-1553-8378
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