ISOLATION, IDENTIFICATION AND PRESERVATION OF PECTINOLYTIC BACTERIA PATHOGENIC TO POTATO



Abstract

Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.


Keywords

Blackleg; Dickeya; Pectobacterium; Polymerase Chain Reaction; selective medium; soft rot

De Boer S. H., Li X., and Ward L. J. 2012. Pectobacterium spp. Associated with bacterial stem rot syndrome of potato in Canada. Phytopathology 102: 937–947.

Degefu Y., Potrykus M., Golanowska M., Virtanen E., Lojkowska E. 2013. A new clade of Dickeya spp. plays a major role in potato blackleg outbreaks in North Finland. Ann. Appl. Biol. 162(2): 231-241.

Duarte V., de Boer S. H., Ward L. J., & de Oliveira A. M. R. 2004. Characterization of atypical Erwinia ca-rotovora strains causing blackleg of potato in Brazil. J. Appl. Microbiology 96: 535–545.

Helias V., Hamon P., Huchet E., van der Wolf J.M., Andrivon D. 2012. Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya. Plant Pathol. 61: 339–345.

Kang H.W., Kwon S.W., Go S.J. 2003. PCR-based specific and sensitive detection of Pectobacterium caroto-vorum ssp. carotovorum by primers generated from a URP-PCR fingerprinting-derived polymorphic band. Plant Pathol. 52: 127–133.

Khayi S., Cigna J., Min Chong T., Quêtu-Laurent A., Chan K.-G., Hélias V., Faure D. 2016. Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. Int. J. Syst. and Evol. Micr. 66: 5379–5383.

Kim M. H., Cho M. S., Kim B. K., Choi H. J., Hahn J. H., Kim C., Kang M. J., Kim S. H., and Park D. S. 2012. Quantitative real-time polymerase chain reaction assay for detection of Pectobacterium wasabiae using YD repeat protein gene-based primers. Plant Dis. 96: 253-257.

Gardan L., Gouy C., Christen R. & Samson R. 2003. Elevation of three subspecies of Pectobacterium caroto-vorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. Int. J. Syst. and Evol. Micr. 53: 381–391.

Oksińska M., Magnucka E., Pietr S. 2016. Fast molecular detection of Pectobacterium atrosepticum, Pecto-bacterium carotovorum subsp. carotovorum, and Dickeya spp. from potato (Solanum tuberosum L.) stem tissues. Wydawnictwo Uniwersytetu Przyrodniczego we Wrocławiu, ISSN 1505-0297, Electronic Jour-nal of Polish Agricultural Universities 19 (4), #09

Potrykus M., Sledz W., Golanowska M., Slawiak M., Binek A., Motyka A., Zoledowska S., Czajkowski R., Lojkowska E. 2014. Simultaneous detection of major blackleg and soft rot bacterial pathogens in potato by multiplex polymerase chain reaction. Ann. Appl. Biol. 165: 474-487.

Pritchard L., Humphris S., Saddler G.S., Parkinson N. M., Bertrand V., Elphinstone J.G., and Toth I.K. 2012. Detection of phytopathogens of the genus Dickeya using a PCR primer prediction pipeline for draft bacterial genome sequences. Plant Pathol. 62: 587-596

Samson R., Legendre J. B., Christen R., Fischer-Le Saux M., Achouak W. & Gardan L. 2005. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. Int. J. Syst. and Evol. Micr. 55 : 1415–1427.

Slawiak M., van Beckhoven J. R. C. M., Speksnijder A. G. C. L., Czajkowski R., Grabe, G. & van der Wolf J. M. 2009. Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe. Eur. J. Plant Pathol. 125: 245–261.

Toth I.K., van der Wolf J.M., Saddler G. et al., 2011. Dickeya species: an emerging problem for potato pro-duction in Europe. Plant Pathol. 60: 385–399

Tsror L., Erlich O., Lebiush S., Hazanovsky M., Zig U., Slawiak M., Grabe G., van der Wolf J. M., & van de Haar J. J. 2009. Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel. Eur. J. Plant Pathol. 123: 311–320.

Tsror L., Erlich O., Hazanovsky M., Ben Daniel B., Zig U., and Lebiush S. 2012. Detection of Dickeya spp. latent infection in potato seed tubers using PCR or ELISA and correlation with disease incidence in commercial field crops under hot-climate conditions. Plant Pathol. 61: 161–168.

van der Wolf, J.M., de Haan, E.G., Kastelein, P., Krijger, M., de Haas, B.H., Velvis, H., Mendes, O., Kooman-Gersmann M., van der Zouwen, P.S. 2017. Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands. Plant Pathol. 66(4):571-583.

van der Wolf J.M., Speksnijder A., Velvis H., van de Haar J., van Doorn J. 2007. Why is Erwinia chrysanthe-mi (Dickeya sp.) taking over? The ecology of a blackleg pathogen. Hannukkala, A, Segerstedt M. New and Old Pathogens of Potato in Changing Climate. Proceedings of the EAPR Pathology Section seminar, 2–6 July 2007, Hattula, Finland.

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Published : 2017-06-20


Lebecka, R. (2017). ISOLATION, IDENTIFICATION AND PRESERVATION OF PECTINOLYTIC BACTERIA PATHOGENIC TO POTATO. Plant Breeding and Seed Science, 75, 87-96. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/215

Renata Lebecka  r.lebecka@ihar.edu.pl
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center; Platanowa Str.19, 05-831 Młochów, Poland  Poland