PLAUSIBLE MECHANISMS BY WHICH ULTRASONIC WAVES AFFECT SEEDS



Abstract

To study the effect of sonication on the seed germination percentage and rate as well as the cell area of barley (Hordeum vulgare L.) a laboratorial experiment was performed as Completely Randomized Design (CRD) with 3 replications. The results indicated that the ultrasonic waves affect the seed germination, germination rate and cell area significantly (at 0.05). The results of the mean comparison tests (LSD, 0.05) showed that the highest germination percentage (100 %), germination rate and cell area (1370.71 µ2 ) is achieved through 15 minutes exposure to ultrasonic waves. It is concluded that weakening the seed’s cell wall rigidity by sonication results in more and faster water imbibition by the cells and improved germination.


Keywords

cell area; barley; germination; seed dormancy breaking; sonication

Barton S., Bullock C., Weir D. 1996 The effects of ultrasound on the activities of some glucosidase enzymes of industrial importance, Enzyme Microb. Technol., vol. 18, pp. 190-194, 1996.

Bewley J.D., Black M. 1994. Seeds. Physiology of Development and Germination. 2nd ed. Plenum Press,New York.

Bradbeer J.W. 1988. Seed dormancy and Germination. Chapman and Hall, New York, USA. 38-54.

Tabatabaie F., Mortazavi A. 2008. Studying the Effects of Ultrasound Shock on Cell Wall Permeability and Survival of Some LAB in Milk. World Applied Sciences Journal 3 (1): 119-121.

Gill P.K., Sharma A.D., Singh P., Bhullar S.S. 2003. Changes in germination, growth and soluble sugar contents of Sorghum bicolor (L.) Moench seeds under various abiotic stresses. Plant Growth Regulation 40: 157–162.

Goussous S.J., Samarah N.H., Alqudah A.M. Othman M.O. 2010. Enhancing seed germination of four crops species using an ultrasonic technique. Experimental Agriculture 46: 231-242.

Gupta A.K., Singh J., Kaur N., Singh R. 1993. Effect of poly-ethylene glycol-induced water stress on uptake, intercon-version and transport of sugars in chickpea seedlings. Plant Physiology and Biochemistry 31: 743–747.

Jambrak A.R., Mason T.J., Paniwnyk L., Lelas V. (2007). Accelerated drying of buttonmushrooms, Brussels sprouts and cauliflower by applying power ultrasound and its rehydrationproperties. Journal of Food Engineering, 81(1), 88-97.

Jun R., Tao L. 2004. Effects of different presowing seed treatments on germination of 10 Calligonum species. Forest Ecology and Management. 195: 291-300.

Kaur S., Gupta A.K., . Kaur N. 2000. Effect of GA3, kinetin and indole acetic acid on carbohydrate metabolism in chickpea seedlings germinating under water stress. Plant Growth Regulation 30: 61–70.

Liu Y., Takatsuki H., Yoshikoshi A., Wang B.C., Sakanishi A. 2003. Effects of ultrasound on the growth and vacuolar H+-ATPase activity of aloe arborescens callus cells,” Colloids and Surfaces B: Biointerfaces, vol. 32, pp. 105-116, 2003.

Mason T.J., Paniwnyk L., Lorimer J.P. 1996. The uses of ultrasound in food technology.Ultrasonics Sonochemistry, 3(3), S253-S260.

Mason T.J., Chemat F., Vinatoru M. 2011. The Extraction of Natural Products Using Ultrasound or Microwaves. Current Organic Chemistry, 15(2), 237-247.

O'Donnell C. P., Tiwari B. K., Bourke P., Cullen P. J. 2010. Effect of ultrasonic processing on food enzymes of industrial importance. Trends in Food Science & Technology, 21(7), 358-367.

Pitt W.G., Ross S.A. 2003. Ultrasound increases the rate of bacterial cell growth, Biotechnol. Prog., vol. 13, pp. 232-237, 2003.

Risca I.M., Fartais L., Stiuca P. 2007. Ultrasound effects contributions on the Norway spruce seeds germination (Picea abies (L.) Karsten), Gen. Biol. Molec., Tom VIII, pp. 87-88, 2007.

Shin Y.K., Baque M.A., Elghamedi S., Lee E.J., Paek K.Y. 2011. Effects of activated charcoal, plant growth regulators and ultrasonic pre-treatments on in vitro germination and protocorm formation of Calanthe hybrids,” AJCS., vol. 5, no. 5, pp. 582-588, 2011.

Sozi G., Chiesa A.O. 1995. Improvement of Caper (Capparis spinosa L.) seed germination by breaking seed coat-induced dormancy. Scientia Horticulture. 62(4): 255-261.

Tiwari B.K., Patras A., Brunton N., Cullen P.J., O'Donnell C.P. 2010. Effect of ultrasoundprocessing on anthocyanins and color of red grape juice. Ultrasonics Sonochemistry, 17(3), 598-604.

Yaldagard M., Mortazavi S.A., Tabatabaie F. 2008a. Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology. 7 (14): 2465-2471.

Yaldagard M., Mortazavi S.A., Tabatabaie F. 2008b. The effect of ultrasound in combination with thermal treatment on the germinated barley’s alpha-amylase activity,” Korean J. Chem. Eng., vol. 25, pp. 517- 523, 2008b.

Zeid I.M., Shedeed Z.A. 2006. Response of alfalfa toputrescine treatment under drought stress. Biologia Plantarum 50: 635–640

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Published : 2016-12-20


Pour, M. E., Hobbi, M., Ghasemi, H., & Nazari, M. (2016). PLAUSIBLE MECHANISMS BY WHICH ULTRASONIC WAVES AFFECT SEEDS. Plant Breeding and Seed Science, 74, 85-92. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/225

Mohammad Eteghadi Pour 
Department of Water and Soil, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran  Iran, Islamic Republic of
Mahsa Hobbi 
Faculty of Chemistry, Islamic Azad University, Tehran, Iran  Iran, Islamic Republic of
Hadi Ghasemi 
Department of Horticultural Sciences, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran  Iran, Islamic Republic of
Meisam Nazari  meisam.nazari1991@gmail.com
F Department of Crop Sciences, Faculty of Agricultural Sciences, Georg-August University of Göttingen, Büsgenweg 5, 37077 Göttingen, Germany; Faculty of Organic Agricultural Sciences, University of Kassel, Nordbahnhofstraße, 37213 Witzenhausen, Germany  Germany