ION-EXCHANGE MEMBRANE CHROMATOGRAPHY AS AN ALTERNATIVE METHOD OF SEPARATION OF POTATO Y VIRUS
Abstract
Procedures of separation of virus particles from a plant material are multistage. Furthermore often they are difficult in terms of methodology and require use of expensive, highly specialist equipment and yield of separation is often low. The antigen obtained is often degraded and contains admixtures of other proteins. Therefore, generation of high quality and specificity antibodies based on such antigen is very difficult and quality of the antibodies has impact on reliability, sensitivity and unambiguity of results of immunodiagnostic tests (e.g. ELISA) that are currently conventionally used to detect vegetable viruses. In this study three conventionallyperformed methods of separation of potato virus Y (PVY) were compared and a method of separation based on membrane chromatography, as an alternative separation technique, has been presented. It has been demonstrated that in proper process conditions good quality virus preparation can be obtained.
Keywords
antigen; membrane techniques; immunodetection; virus purification
References
Burden C.S., Jin J., Podgornik A., Bracewell D.G. 2011. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 880: 82.
Cerovská N., Filigarová M., Šubr Z. 1997. Optimalization of purification procedure for potato virus Y strain NN. Acta Virologica 41(1): 47–49.
Chrzanowska M. 1991. New isolates of the necrotic strain of Potato virus Y (PVY N) found recently in Poland. Potato Res. 34: 179-182.
Clark M. F., Adams A. M. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483.
Downing, L.A., Bernstein, J.M., Walter, A. 1992. Active respiratory syncytial virus purified by ion-exchange chromatography: characterization of binding and elution requirements. J. Virol. Methods 38: 215–228.
Ghosh R. 2002. Protein separation using membrane chromatography: opportunities and challenges. J. Chromatogr. A. 952: 13.
Grunwald A.G., Shields M.S. 2001. Plasmid Purification Using Membrane-Based Anion-Exchange Chromatography. Anal. Biochem. 296(1): 1–4.
Hammond J., Lawson R.H. 1988. An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissues. Journal of Virological Methods 20: 203-217.
Hühnlein A., Drechsler N., Steinbach P., Thieme T., Schubert J. 2013 Comparison of three methods for the detection of Potato virus Y in seed potato certification. J Plant Dis Protection 120: 57 69.
Karger A., Bettin B., Granzow H., Mettenleiter T.C. 1998. Simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane. J. Virol. Methods 70: 219–224.
Kovac K., Gutiérrez-Aguirre I., Banjac M., Peterka M., Poljsak-Prijatelj M., Ravnikar M., Mijovski J.Z., Schultz A.C., Raspor P. 2009. A novel method for concentrating hepatitis A virus and caliciviruses from bottled water. J. Virol. Methods, 162: 272.
Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259): 680-685.
Nochumson S., Zhang S. Rapid Culture and Elution of Large Quantities of Plasmid DNA (100 mg) Using Anion Exchange Chromatography Membranes. Fifth Meeting of the American Society of Gene Therapy, 5 June 2002, Abstract No. 1031.
Paul H. L. 1975. SDS-polyacrylamide gel electrophoresis of virion proteins as a tool for detecting the presence of virus in plants. II Examination of further virus-host combinations. Phtyopath. Z. 83: 303 – 310.
Roudet-Tavert G., German-Retana S., Delaunay T., Delécolle B., Candresse T., Le Gall O. 2002. Interaction between potyvirus helper component-proteinase and capsid protein in infected plants. J. Gen. Virol. 83: 1765.
Rupar M., Ravnikar M., Tušek-Žnidari M., Kramberger P., Glais L., Gutiérrez-Aguirre I. 2013. Fast purification of the filamentous Potato virus Y using monolithic chromatographic supports. J Chromatogr. A. 1272: 33-40.
Singh R. P. 1978. Detection of Potato Viruses by Electrophoresis on Polyacrylamide Gels. Report of Planning Conference on Development in the control of potato virus diseases. International Potato Center. CIP, Lima, Peru. 130-137.
Singh R.P., McLaren D.L., Nie X., Singh M. 2003. Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection. Plant Dis 87: 679-685.
Smrekar F., Ciringer M., Peterka M., Podgornik A., Strancar A. 2008. Purification and concentration of bacteriophage T4 using monolithic chromatographic supports. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 861: 177.
Thornbury D.W., Hellmann G.M., Rhoads R.E., Pirone T.P. 1985. Purification and characterization of potyvirus helper component. Virology. 144: 260–267.
Walin, L., Tuma, R., Thomas, G.J. Jr., Bamford, D.H. 1994. Purification of viruses and macromolecular assemblies for structural investigations using a novel ion exchange method. Virology 201:1–7.
Wróbel S. 2014. Modification of ELISA by replacing incubation of microtiter plates in an incubator with their shaking in PVY, PVM and PLRV detection. American Journal of Potato Research 91(4): 354 – 362.
Valkonen J. P. 2007. Viruses: Economical losses and bio - technological potential. In: Vreugdenhil D, Bradshaw J, Gebhardt C, Govers F, McKarren DKL, Taylor MA & Ross HA (Eds.). Potato biology and biotechnology – Advances and perspectives. Elsevier 619-641.
Vincente T., Sousa M., Peixoto C., Mota J., Alves P., Carrondo M. 2008. Anion-exchange membrane chromatography for purification of rotavirus-like particles. Journal of Membrane Science 311: 270-283.
Zahn V. 2004. Altered occurrence of viruses. Results of potato virus testing by LWK Hannover. Kartoffelbau 55: 394-397.
Zhou, J. X. and Tressel, T. (2006), Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production. Biotechnol Progress, 22: 341–349.
Zhou J.X., T. Tressel, U. Gottschalk, F. Solamo, A. Pastor, S. Dermawan, Hong T., Reif O., Mora J., Hutchison F., Murphy M. 2006. New Q membrane scale-down model for process-scale antibody purification, J. Chromatogr. A 1134: 66-73.
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Warsaw University of Life Sciences-SGGW, Faculty of Agriculture and Biology, Dept. of Botany, Nowoursynowska ST 159, 02-776 Warsaw Poland
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