Genetic diversity of common bean genotypes as revealed by seed storage proteins and some agronomic traits



Abstract

Evaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 com- mon bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.

Positive correlations were observed among both seed morphological characters and developmental charac- ters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain  morphological  traits  resulted  in three factors  were named yield  components,   seed morphology  and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.

Nei’s diversity coefficient (He = 0.4), effective number of alleles (Ae  = 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common  bean are different in seed size, the result of GST statistics showed that bands with relative mobility of 30, 32, 38 and 40 differen- tiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.


Keywords

agronomic traits; genetic diversity; Phaseolus vulgaris L.; SDS-PAGE; seed storage proteins

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Published : 2012-12-20


Marzooghian, A., Moghaddam, M., Valizadeh, M., & Kooshki, M. H. (2012). Genetic diversity of common bean genotypes as revealed by seed storage proteins and some agronomic traits. Plant Breeding and Seed Science, 67, 125-137. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/310

Akbar Marzooghian  marzooghian@tabrizu.ac.ir
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran  Iran, Islamic Republic of
Mohammad Moghaddam 
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran  Iran, Islamic Republic of
Mostafa Valizadeh 
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran  Iran, Islamic Republic of
Mohammad Hasan Kooshki 
Broujerd Agricultural Research Station, Broujerd, Iran  Iran, Islamic Republic of