Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions.

A. Prodi

antonio.prodi@unibo.it
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

D. Salomoni


Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)

E. Bertacchini


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

D. Alkadri


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

A. Pisi


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

S. Tonti


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

I. Alberti


INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

M. Dal Prà


INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

D. Pancaldi


Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)

L. Covarelli


Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. (Italy)

P. Nipoti


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)


Abstract

Durum wheat production in Italy is economically of great importance. Fusarium graminearum is the main fusarium head blight (FHB) causal agent in wheat, reducing both yield and grain quality. F. graminearum produces several mycotoxins and, among trichothecenes, deoxynivalenol (DON) and nivalenol (NIV) are the most studied for their toxicity towards humans and animals. DON-producing isolates can be further distin- guished on the basis of the predominant acetyl-DON derivative in 3-acetyldeoxynivalenol (3-ADON) or 15- acetyldeoxynivalenol (15-ADON). In order to evaluate possible mycotoxin contamination risks in food, it is very important to know which chemotype is the prevalent in a F. graminearum population.

F. graminearum sensu stricto strains were collected from symptomatic durum wheat heads and grains of several naturally infected fields located mostly in Emilia – Romagna, The Marche, Lazio, Tuscany and Umbria. A multiplex PCR in the region of genes Tri12, located in the terminal gene cluster of trichothecenes, was used to characterize 187 single-spore isolates of F. graminearum as NIV, 3-ADON and 15-ADON chemotypes.

All the three chemotypes were present in the F. graminearum population studied. The most frequent chemotype was 15-ADON (83.4%), followed by 3-ADON (10.7%) and NIV (5.9%). NIV-producing isolates were found only in Emilia-Romagna (3.5%), Umbria (33.3%) and The Marche (5.7%).


Keywords:

DON and NIV chemotypes, durum wheat, Fusarium Head Blight, Tri12

Balmas V., Vitale S., Marcello A., Corazza L. 2000a La fusariosi della spiga. L’informatore Agrario 35 Supplemento: 27-29.
Google Scholar

Balmas V., Santori A., Corazza L. 2000b Le specie di Fusarium più comuni in Italia. Petria 10, suppl. 1: 1-60. Bottalico A., Perrone G. (2002) Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. Eur J of Plant Pathol 108: 611–624.
Google Scholar

Gale L.R., Ward T.J., Balmas V., Kistler H.C. 2007 Population subdivision of Fusarium graminearum sensu stricto in the upper Midwestern United States. Phytopathology 97: 1434-1439.
Google Scholar

Guo X.W., Fernando W.G.D., Seow-Brock H.Y. 2008 Population structure, chemotype diversity, and potential chemotype shifting of Fusarium graminearum in wheat fields of Manitoba. Plant Disease 92 (5): 756 -762.
Google Scholar

Jennings P., Coates M.E., Walsh K., Turner J.A., Nicholson P. 2004 Determination of deoxynivaleonl- and nivalenol-producing chemiotypes of Fusarium graminearum isolated from wheat crops in England and Wales. Plant Pathology 53: 643-652.
Google Scholar

Ji L., Cao K., Hu T., Wang S. 2007 Determination of Deoxynivalenol and Nivalenol chemotypes of Fusarium graminearum isolates from China by PCR assay. Journal of Phytopathology 155: 505-512.
Google Scholar

Leslie J.F., Summerell B.A. 2006 The Fusarium Laboratory Manual. Blackwell Publishing, Ames, U.S.A: pp. 388
Google Scholar

Lohdi M.A., Ye G., Weeden F., Reisch B.I. 1994 A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter 12: 6-13.
Google Scholar

Lops R., Pascale M., Pancaldi D., Visconti A. 1998. Infezioni fungine e presenza di deossinivalenolo in cariossidi di frumento prodotte in diverse regioni italiane. Inf.tore fitopatologico 48 (4): 60-66.
Google Scholar

Miller J.D., Greenhalgh R., Wang Y.Z., Lu M. 1991 Trichothecene chemotypes of three Fusarium species. Mycologia 83: 121-130.
Google Scholar

Nicholson P., Simpson D.R., Weston G., Rezanoor H.N., Lees A.K., Parry D.W., Joyce D. 1998 Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 53: 17-37.
Google Scholar

Pancaldi D., Grazzi G., Alberti I. 1996 La fusariosi della spiga di frumento in Emilia Romagna nel 1995. Informatore fitopatologico 46 (4): 16-20.
Google Scholar

Pancaldi D., Pisi A., Alberti I., Romani S. 2004 Control of Fusarium head blight and accumulation of deoxynivalenol in durum wheat grain, semolina and bran. Phytopathol Medit 43: 351-359.
Google Scholar

Pancaldi D., Tonti S., Prodi A., Salomoni D., Dal Prà M, Nipoti P., Alberti I., Pisi A. 2010. Survey of the main causal agents of fusarium head blight of durum wheat – Bologna - Northern Italy. Phytopathologia mediterranea 49 (2), 258-266
Google Scholar

Parry D.W., Jenkinson P., McLeod L. 1995 Fusarium ear blight (scab) in small grains - a review. Plant Pathol. 44: 207–238.
Google Scholar

Pascale M., Bottalico A., Pancaldi D., Perrone G., Visconti A. 2002 Occurrence of deoxynivalenol in cereals from experimental fields in various Italian regions. Petria 12, 123-129.
Google Scholar

Pasquali M., Giraud F., Brochot C., Cocco E., Hoffmann L., Bohn T. 2010 Genetic Fusarium chemotyping as a useful tool for predicting nivalenol contamination in winter wheat Int J Food Microbiol. 137, 246-253.
Google Scholar

Peglion V. 1900 Sulla cosidetta “Golpe Bianca” del frumento. Bollettino Notiziario Agrario 20, 17-26.
Google Scholar

Prodi A., Tonti S., Nipoti P., Pancaldi D., Pisi A. 2009 Identification of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolates from durum wheat in a restricted area of northern Italy. J of Plant Pathol 91 (3): 611-615.
Google Scholar

Qu B., Li H.P., Zhang J.B., Xu J.B., Huang T., Wu A.B., Zhao C.S., Carter J., Nicholson P., Liao Y.C. 2008. Geographic distribution and genetic diversity of Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology 57: 15-24.
Google Scholar

Quarta A., Mita G., Haidukowski M., Santino A., Mulè G., Visconti M. 2005 Assesment of trichothecene chemotypes of Fusarium culmorum occurring in Europe. Food Additives and Contaminants 22(4): 309– 315.
Google Scholar

Rossi V., Giosuè S., Cigolini M., Delogu G., Faccini N., Terzi V., Scudellari D. 2006 Un aiuto alla gestione della fusariosi della spiga. L’Inf.tore Agrario, 62 (14): 62-68.
Google Scholar

Rossi V. 2008 La Fusariosi della spiga: una annata favorevole alla malattia che conferma però l’affidabilità dei sistemi di previsione. Filiera Grano duro news 8: 2.
Google Scholar

Shah D.A., Pucci N., Infantino A. 2005. Regional and varietal differences in the risk of wheat seed infection by fungal species associated with fusarium head blight in Italy. Eur J of Plant Pathol 112: 13-21.
Google Scholar

Starkey D.E., Ward T.J., Aoki T., Gale L.R., Kistler H.C., Geiser D.M., Suga H., Toth B., Varga J., O’Donnell K. 2007. Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal genetics and biology 44(11): 1191-1204.
Google Scholar

Ohe C.,von der Gaunther V., Tamburic-Illincic L., Brule-Babel A., Fernando W.G.D., Clear r., Ward T.J., Miedaner T. 2010 A comparison of aggressiveness and Deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat. Eur J Plant Pathol 127: 407-417.
Google Scholar

Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O’Donnell K. 2002. Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium. Proceedings of the National Academy of Sciences of the USA 99: 9278-9283.
Google Scholar

Ward T.J., Clear R.M., Rooney A.P., O’Donnell K., Gaba D., Patrick S., Starkey D.E., Gilbert J., Geiser D.M., Nowicki T.W. 2008. An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America. Fungal Genetics and Biology 45: 473-484.
Google Scholar

Yli-Mattila T., O’Donnell K., Ward T., Gagkaeva T. 2008. Trichotecene chemotype composition of Fusarium graminearum and related species in Finland and Russia. J of Plant Pathol 90 (3): S 3.60.
Google Scholar

Download


Published
2011-12-20

Cited by

Prodi, A. ., Salomoni, D., Bertacchini, E., Alkadri, D. ., Pisi, A. ., Tonti, S., … Nipoti, P. . (2011). Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions. Plant Breeding and Seed Science, 64, 75–80. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/344

Authors

A. Prodi 
antonio.prodi@unibo.it
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

D. Salomoni 

Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy

Authors

E. Bertacchini 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

D. Alkadri 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

A. Pisi 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

S. Tonti 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

I. Alberti 

INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

M. Dal Prà 

INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

D. Pancaldi 

Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy

Authors

L. Covarelli 

Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. Italy

Authors

P. Nipoti 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Statistics

Abstract views: 107
PDF downloads: 50


License

All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .