Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions.
A. Prodi
antonio.prodi@unibo.itDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)
D. Salomoni
Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)
E. Bertacchini
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)
D. Alkadri
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)
A. Pisi
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)
S. Tonti
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)
I. Alberti
INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)
M. Dal Prà
INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)
D. Pancaldi
Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)
L. Covarelli
Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. (Italy)
P. Nipoti
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)
Abstrakt
Durum wheat production in Italy is economically of great importance. Fusarium graminearum is the main fusarium head blight (FHB) causal agent in wheat, reducing both yield and grain quality. F. graminearum produces several mycotoxins and, among trichothecenes, deoxynivalenol (DON) and nivalenol (NIV) are the most studied for their toxicity towards humans and animals. DON-producing isolates can be further distin- guished on the basis of the predominant acetyl-DON derivative in 3-acetyldeoxynivalenol (3-ADON) or 15- acetyldeoxynivalenol (15-ADON). In order to evaluate possible mycotoxin contamination risks in food, it is very important to know which chemotype is the prevalent in a F. graminearum population.
F. graminearum sensu stricto strains were collected from symptomatic durum wheat heads and grains of several naturally infected fields located mostly in Emilia – Romagna, The Marche, Lazio, Tuscany and Umbria. A multiplex PCR in the region of genes Tri12, located in the terminal gene cluster of trichothecenes, was used to characterize 187 single-spore isolates of F. graminearum as NIV, 3-ADON and 15-ADON chemotypes.
All the three chemotypes were present in the F. graminearum population studied. The most frequent chemotype was 15-ADON (83.4%), followed by 3-ADON (10.7%) and NIV (5.9%). NIV-producing isolates were found only in Emilia-Romagna (3.5%), Umbria (33.3%) and The Marche (5.7%).
Słowa kluczowe:
DON and NIV chemotypes, durum wheat, Fusarium Head Blight, Tri12Bibliografia
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Autorzy
A. Prodiantonio.prodi@unibo.it
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy
Autorzy
D. SalomoniDepartment of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy
Autorzy
E. BertacchiniDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy
Autorzy
D. AlkadriDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy
Autorzy
A. PisiDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy
Autorzy
S. TontiDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy
Autorzy
I. AlbertiINRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy
Autorzy
M. Dal PràINRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy
Autorzy
D. PancaldiDepartment of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy
Autorzy
L. CovarelliDipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. Italy
Autorzy
P. NipotiDepartment of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy
Statystyki
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