Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions.

A. Prodi

antonio.prodi@unibo.it
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

D. Salomoni


Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)

E. Bertacchini


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

D. Alkadri


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

A. Pisi


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)

S. Tonti


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

I. Alberti


INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

M. Dal Prà


INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. (Italy)

D. Pancaldi


Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. (Italy)

L. Covarelli


Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. (Italy)

P. Nipoti


Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. (Italy)


Abstract

Durum wheat production in Italy is economically of great importance. Fusarium graminearum is the main fusarium head blight (FHB) causal agent in wheat, reducing both yield and grain quality. F. graminearum produces several mycotoxins and, among trichothecenes, deoxynivalenol (DON) and nivalenol (NIV) are the most studied for their toxicity towards humans and animals. DON-producing isolates can be further distin- guished on the basis of the predominant acetyl-DON derivative in 3-acetyldeoxynivalenol (3-ADON) or 15- acetyldeoxynivalenol (15-ADON). In order to evaluate possible mycotoxin contamination risks in food, it is very important to know which chemotype is the prevalent in a F. graminearum population.

F. graminearum sensu stricto strains were collected from symptomatic durum wheat heads and grains of several naturally infected fields located mostly in Emilia – Romagna, The Marche, Lazio, Tuscany and Umbria. A multiplex PCR in the region of genes Tri12, located in the terminal gene cluster of trichothecenes, was used to characterize 187 single-spore isolates of F. graminearum as NIV, 3-ADON and 15-ADON chemotypes.

All the three chemotypes were present in the F. graminearum population studied. The most frequent chemotype was 15-ADON (83.4%), followed by 3-ADON (10.7%) and NIV (5.9%). NIV-producing isolates were found only in Emilia-Romagna (3.5%), Umbria (33.3%) and The Marche (5.7%).


Keywords:

DON and NIV chemotypes, durum wheat, Fusarium Head Blight, Tri12

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Published
2011-12-20

Cited by

Prodi, A. ., Salomoni, D., Bertacchini, E., Alkadri, D. ., Pisi, A. ., Tonti, S., … Nipoti, P. . (2011). Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions. Plant Breeding and Seed Science, 64, 75–80. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/344

Authors

A. Prodi 
antonio.prodi@unibo.it
Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

D. Salomoni 

Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy

Authors

E. Bertacchini 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

D. Alkadri 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

A. Pisi 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

Authors

S. Tonti 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy, and INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

I. Alberti 

INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

M. Dal Prà 

INRAN (ex ENSE), Via Ca’ Nova Zampieri 37, 37057 S. Giovanni in Lupatoto (VR), Italy. Italy

Authors

D. Pancaldi 

Department of Agri-food Protection and Improvement, Alma Mater Studiorum Universityof Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Italy

Authors

L. Covarelli 

Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy. Italy

Authors

P. Nipoti 

Department of Agroenvironmental Science and Technology – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy. Italy

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