Biomolecular characterization of Fusarium poae strains isolated from durum wheat in central Italy.
I. Alberti
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
Dal M. Prà
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
S. Tonti
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
M. Montanari
ENSE, Via Sicilia 2, Osteria Grande, Bologna, Italy. (Italy)
A. Prodi
Department of Agroenvironmental Science and Technology (DiSTA) – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale Fanin 40, Bologna, Italy. (Italy)
D. Pancaldi
Department of Agri-food Protection and Improvement (DIPROVAL), Alma Mater Studiorum University of Bologna, Viale Fanin 46, Bologna, Italy. (Italy)
Abstract
Fusarium Head Blight (FHB) is a worldwide disease affecting wheat, barley and other grains, reducing kernel weight and grain yield; infected seeds may contain a large number of mycotoxins, including trichothe- cenes of type A and B. These compounds have already been associated with human and animal toxicoses.
Most common species causing the disease are F. graminearum, F. culmorum and F. avenaceum, but in the last few years a gradual increase in incidence of another species, F. poae, has been reported. In general terms, F. poae is a relatively weak pathogen, but its contribute to the increase of mycotoxins level still has to be clarified.
Durum wheat is widely cultivated in the central part of Italy, however the effective incidence of F. poae in this area still has to be investigated.
In order to monitor Fusarium risk, we collected dozens of F. poae strains on seeds and glumes of durum wheat coming from some of the most important cultivated areas of Central Italy. Every isolate was identified both by microscope observation and by PCR assay with the primer pair Fp82 F/R.
Strains were therefore subjected to a more accurate molecular characterization by Translation Elongation Factor 1-alpha (TEF-1α) gene sequencing.
Keywords:
durum wheat, FHB, F. poae, gene sequencing, mycotoxins, trichothecenesReferences
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Authors
I. AlbertiENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Authors
Dal M. PràENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Authors
S. TontiENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Authors
M. MontanariENSE, Via Sicilia 2, Osteria Grande, Bologna, Italy. Italy
Authors
A. ProdiDepartment of Agroenvironmental Science and Technology (DiSTA) – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale Fanin 40, Bologna, Italy. Italy
Authors
D. PancaldiDepartment of Agri-food Protection and Improvement (DIPROVAL), Alma Mater Studiorum University of Bologna, Viale Fanin 46, Bologna, Italy. Italy
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