EVALUATION OF RESISTANCE TO POTATO VIRUS Y OF CULTIVARS IN PRE-REGISTRATION (STATUTORY) TRIALS
Krystyna Michalak
k.michalak@ihar.edu.plPlant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, 19, Platanowa Str., 05-831 Młochów, Poland; (Poland)
Mirosława Chrzanowska
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, 19, Platanowa Str., 05-831 Młochów, Poland (Poland)
Abstrakt
The paper describes testing potato cultivars for resistance to potato viruses in field and greenhouse condi-tions according to the scheme of the statutory trials. Seed tubers of tested cultivar are planted in the field in two replications with 30 tubers each. On both sides of each cultivar, 15 PVY-infected tubers, i.e. the source for PVY inoculation, are planted. The evaluation includes standard cultivars of known resistance levels to PVY. The progeny tubers are collected in autumn and growing-on testing is carried out in greenhouse condi-tions to estimate the number of plants infected with PVY. The other method is to evaluate the resistance of potato cultivars in greenhouse conditions. At present, the resistance of cultivars is evaluated only with respect to PVY, the most important potato virus. Two strains are used in the evaluation, i.e. PVYN-Wi and PVYNTN. For each testing cultivar, 10 plants are mechanically inoculated with each PVY strain. The analysis of the results makes it possible to classify the cultivars into four resistance groups on a 1-9 scale: highly resistant (with the resistance score of 8), resistant (with a value of 7), mid-resistant (with a value 5-6), and susceptible (with a value of 3-4). The ”9” rating is specified after the registration of the cultivars based on molecular tests, that validate the presence of a marker linked to extreme resistance (ER) gene Rysto.
Słowa kluczowe:
field test, growing-on test, greenhouse test, potato cultivar, PVYBibliografia
Chrzanowska M., Doroszewska T. 1997. Comparison between PVY isolates obtained from potato and tobacco plants grown in Poland. Phytopathol. Polonica 13: 63-71.
Google Scholar
Chrzanowska M., Michalak K., Zagórska H., Szajko K. 2011. Reakcja na wirusy odmian ziemniaka znajdują-cych się w Krajowym Rejestrze w 2010 roku. Biuletyn IHAR nr 260/261, 309 – 324.
Google Scholar
Gabriel W. 1995. Une methode d`utilisation possible de 1 echelle de 1 a 9 pour l`evaluation de la resistance aux virus chez les cultivars de pomme de terre. Potato Res. 38: 297-305.
Google Scholar
Syller J. 2001. The enzyme-linked immunosorbent assay (ELISA) procedure. Monografie i rozprawy nau-kowe 10a/2001 IHAR Radzików: 21-23.
Google Scholar
Turska E. 1971. Przerywanie okresu spoczynku nowych odmian i niektórych rodów hodowlanych. Z prac Inst. Ziemn. 2: 9-16.
Google Scholar
Wójcik A.R., Gabriel W., Woźnica W. 1976. Metody transformowania danych procentowych porażenia w epidemiologii wirusów ziemniaka. Biul. Inst. Ziemn. 17: 83-107.
Google Scholar
Autorzy
Krystyna Michalakk.michalak@ihar.edu.pl
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, 19, Platanowa Str., 05-831 Młochów, Poland; Poland
Autorzy
Mirosława ChrzanowskaPlant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, 19, Platanowa Str., 05-831 Młochów, Poland Poland
Statystyki
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Licencja
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