DETECTION OF POTATO VIRUS Y (PVY) BY REVERSE-TRANSCRIPTION LOOP-MEDIATED NUCLEIC ACID AMPLIFICATION (RT-LAMP)

Krzysztof Treder

k.treder@ihar.edu.pl
Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland (Poland)

Joanna Chołuj


Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland (Poland)

Bogumiła Zacharzewska


Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland (Poland)

Mateusz Mielczarek


Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland (Poland)


Abstrakt

Potato virus Y (PVY), a type member of the genus Potyvirus (family Potyviridae), is currently the most important virus infecting the potato crop. PVY is also a dangerous pathogen of the tomato, pepper, and tobac-co. The reverse transcription loop-mediated amplification (RT-LAMP) is gaining recognition as a good alter-native to RT-PCR in diagnosing plant viruses. Here, we provide a detailed description of a simple protocol for fast and sensitive detection of PVY by the RT-LAMP assay, which can be easily adapted to detect other plant pathogens, harboring both RNA and DNA genomes.


Słowa kluczowe:

detection, plant pathogen, RT-LAMP

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Opublikowane
2017-06-20

Cited By / Share

Treder, K., Chołuj, J., Zacharzewska, B., & Mielczarek, M. (2017). DETECTION OF POTATO VIRUS Y (PVY) BY REVERSE-TRANSCRIPTION LOOP-MEDIATED NUCLEIC ACID AMPLIFICATION (RT-LAMP). Plant Breeding and Seed Science, 75, 77–85. Pobrano z http://ojs.ihar.edu.pl/index.php/pbss/article/view/214

Autorzy

Krzysztof Treder 
k.treder@ihar.edu.pl
Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland Poland

Autorzy

Joanna Chołuj 

Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland Poland

Autorzy

Bogumiła Zacharzewska 

Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland Poland

Autorzy

Mateusz Mielczarek 

Plant Breeding and Acclimatization Institute - National Research Institute, Bonin Research Center, Laboratory of Molecular Diagnostic and Biochemistry;76-009 Bonin, Poland Poland

Statystyki

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PDF downloads: 80


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