Analysis of the impact of pollination method on the results of mapping QTL for pre-harvest sprouting in rye
Beata Myśków
beata.myskow@zut.edu.plKatedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie (Poland)
Anna Łań
Katedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie (Poland)
Monika Hanek
Katedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie (Poland)
Abstract
Rye mapping population of S120×S76 cross consisting of 143 genotypes of RIL-F8 generation and it has been used to analyze pre-harvest sprouting. The map of seven rye chromosomes was together 962 cM long and contained 1285 DArT and 62 PCR markers. Plant material was grown in an experimental field of the West Pomeranian University of Technology in Szczecin, Poland, in four years (2007–2010). PHS was measured as a percentage of germinating seeds per total number of seeds in the ear, after watering of mature, harvested spikes. Each RIL was represented by several self-pollinated spikes (6 for most genotypes) every season and by several open-pollinated spikes in two years (2008, 2010). The objective of the research was to determine the positions of QTL for pre-harvest sprouting on rye genetic map of RIL population using spikes of two types of pollinating and comparing them together. There were jointly 33 QTL for PHS detected, that were spread on all chromosomes. Data from 2008 based on isolated and non-isolated spikes allowed to map 12 different (six for each variant) and one coinciding QTL. There was one QTL detected with the use of isolated spikes in 2010 and it overlapped with one of six QTL revealed on the basis of non-isolated spikes in this year. Localization of some of these QTL was confirmed by QTL analysis from other seasons. There was no overlapping QTL between 2008 and 2010, regardless the method of plant pollinating. It was suggested that open-pollinated spikes could be used to study QTL for PHS equally well as the self-pollinated plants or as a complementary plant material.
Supporting Agencies
Keywords:
open-pollination, pre-harvest sprouting (PHS), Quantitative Trait Loci (QTL), rye, self-pollinationReferences
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Authors
Beata Myśkówbeata.myskow@zut.edu.pl
Katedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie Poland
Authors
Anna ŁańKatedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie Poland
Authors
Monika HanekKatedra Genetyki, Hodowli i Biotechnologii Roślin, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie Poland
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