Variability of quantitative traits of two mutated grasspea (Lathyrus sativus L.) cultivars
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Jan Bocianowski
Katedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Katarzyna Pankiewicz
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Abstract
Grasspea (Lathyrus sativus L.) is cultivated in Poland (Podlasie and adjacent regions) since 200 years. Although this pulse crop is characterized by high protein content (26-34%) and unique resistance against abiotic stresses, grasspea plays a marginal role in Polish agriculture. This indicates, among others, the necessity of genetic improvement of this crop to make grasspea more competitive as compared to others pulse crops. A local population of grasspea present in the Podlasie region since many years is characterized by narrow gene pool which limits effective selection in breeding works leading to release improved cultivars. Recombination but mainly induced mutations may enrich the existing grasspea materials with an additional variability of traits important in agriculture. As a result of mutagenic treatment of seeds of two Polish cultivars: Derek and Krab (derived from a local Podlasie population) with chemomutagens, in M2 progeny many changed plants were selected. After propagation of obtained lines in next generation, part of them constituted initial material for field trials conducted in 2004–2006. The aim of this work was an estimation of quantitative traits variations of mutants in comparison to initial cultivars. The results obtained from field trials were analyzed with a use of multivariate statistics. The two-way analysis of variance was carried out to determine the effects of objects, years and objects years interaction on the variability of observed phenotypic traits. The mean values and coefficient of variation were estimated. For jointly analyzed results from all years, the highest coefficients of variation were obtained for plant height, number of pods per plant, number and seed weight per plant and 1000 seed weight, and the lowest for flowering times, seeds number per pod and length and broad of pod. The values of contrast in comparisons between initial cultivars (control) and mutants in the most cases were positive which indicated higher values of traits for cultivars. Based on Mahalnobis distances the similarity of particular forms in comparison to cultivars for all traits together were estimated. The forms with highest and lowest similarity to cultivars were distinguished. Part of them, characterized by desirable traits from breeding point of view, can constitute a valuable initial material for further work on genetic improvement of domestic grasspea.
Keywords:
Lathyrus sativus, field trials, multivariate analysis, mutants, quantitative traitsReferences
Ahloowalia B. S, Małuszyński M. 2001. Induced mutations — a new paradigm in plant breeding. Euphytica 118: 167 — 173.
Google Scholar
Ahloowalia B. S., Małuszyński M., Nitcherlein K. 2004. Global impact of mutation — derived varieties. Euphytica 135: 187 — 204.
Google Scholar
Allkin R., Macfarlane T. D., White F. A., Bisby F. A., Adey M. E. 1983. Names and synonyms of species and subspecies in Vicieae. Issue 2, Vicieae Database Project Publication No. 2, Southampton, UK.
Google Scholar
Bocianowski J., Liersch A., Bartkowiak-Broda I. 2009. Badanie zmienności fenotypowej mieszańców F1 CMS ogura rzepaku ozimego i ich form rodzicielskich za pomocą statystycznych metod wielowymiarowych. Rośliny Oleiste — Oilseed Crops XXX: 161 — 184.
Google Scholar
Bocianowski J., Rybiński W. 2006. Wykorzystanie wielowymiarowej metodyki oceny cech ilościowych mutantów lędźwianu siewnego (Lathyrus sativus L.) uzyskanych działaniem chemomutagenów i światła lasera helowo-neonowego. Acta Agrophysica 8 (4): 791 — 802.
Google Scholar
Bocianowski J., Rybiński W. 2008. Wykorzystanie analizy zmiennych kanonicznych do wielocechowej charakterystyki dwurzędowych i wielorzędowych linii DH jęczmienia jarego (Hordeum vulgare L.). Annales UMCS, Sectio E: Agricultura LXIII (3): 53 — 61.
Google Scholar
Bocianowski J., Skomra U. 2008. Wykorzystanie analizy zmiennych kanonicznych do wielocechowej charakterystyki odmian chmielu zwyczajnego (Humulus lupulus L.). Pamiętnik Puławski 148: 107 — 118.
Google Scholar
Bocianowski J., Stokłosa A. 2010. Ocena kiełkowania odmian botanicznych owsa głuchego (Avena fatua L.) w różnych warunkach świetlnych i termicznych za pomocą analizy zmiennych kanonicznych. Nauka Przyroda Technologie 4: 60 — 65.
Google Scholar
Caliński T., Kaczmarek Z. 1973. Metody kompleksowej analizy doświadczenia wielocechowego. Colloquium Metodologiczne z Agrobiometrii, PAN i PTB, Warszawa, 3: 258 — 320.
Google Scholar
Campbell C. G., Mehra R. B., Agrawal S. K., Chen, Y. Z., Abd El Moneim A. M., Khawaja H. I. T., Yadow, C. R., Tay, J. U., Araya, W. A. 1994. Current status and future strategy in breeding grasspea (Lathyrus sativus L.). Euphytica 73: 167 — 175.
Google Scholar
Campbell C. G. 1997. Promoting the conservation and use of underutilized and neglected crops. 18. Institute of Plant Genetics and Crop Plant Research. Gatersleben/International Plant Genetic Resources Institute, Rome, Italy.
Google Scholar
Camussi A., Ottaviano E., Caliński T., Kaczmarek Z. 1985. Genetic distances based on quantitative traits. Genetics 111: 945 — 962.
Google Scholar
Dziamba S. 1997. Biologia i agrotechnika lędźwianu siewnego. Międzynarodowe Sympozjum Naukowe „Lędźwian siewny — agrotechnika i wykorzystanie w żywieniu zwierząt i ludzi”, Radom, 9–10 czerwca 1997: 27 — 33.
Google Scholar
GenStat 2007. GenStat Release 10 Reference Manual, Lawes Agricultural Trust Rothamsted.
Google Scholar
Grela E. R., Rybiński W., Klebaniuk R., Matras J. 2010. Morphological characteristics of some accessions of grasspea (Lathyrus sativus L.) grown in Europe and nutritional traits of their seeds. Genet. Resour. Crop. Evol. 57 (5): 693 — 701.
Google Scholar
Hanbury C. D., Sarker A., Siddique K. H. M., Perry M. W. 1995. Evaluation of Lathyrus germplasm in a Mediterranean type environment in South-Western Australia. Cooperative Research Center for Legumes in Mediterranean Agriculture, Occasional Paper, No 8. Perth.
Google Scholar
Kislev M. E. 1986. Archeobotanical findings of the origin of Lathyrus sativus and L. cicera. In: Lathyrus and Lathyrism (Eds. Kaul A.K. and Combes D.). Third World Medical Research Foundation, New York: 46 — 51.
Google Scholar
Mahalanobis P. C. 1936. On the generalized distance in statistics. Proceedings of the National Institute of Science of India 12: 49 — 55.
Google Scholar
Milczak M., Pędziński M., Mnichowska H., Szwed-Urbaś K. 1997. Hodowla twórcza lędźwianu siewnego (Lathyrus sativus L.) — podsumowanie pierwszego etapu. Międzynarodowe Sympozjum Naukowe „Lędźwian siewny — agrotechnika i wykorzystanie w żywieniu zwierząt i ludzi”. Radom, 9–10 czerwca 1997: 13 — 22.
Google Scholar
Milczak M., Pędziński M., Mnichowska H., Szwed-Urbaś K., Rybiński W. 2001. Creative breeding of grasspea (Lathyrus sativus L.) in Poland. Lathyrus Lathyrism Newsletter 2: 18 — 23.
Google Scholar
Pandey R. L., Agraval S. K., Chitale M. W., Sharma R. N., Kashyap O. P., Geda A. K., Chandrakar H. K., Agrawal K. K. 1995. Catalogue of grasspea (L. sativus L.) germplasm. I. Gandhi Agric. Univ. Press, Raipur, India.
Google Scholar
Rencher A. C. 1992. Interpretation of canonical discriminate functions, canonical varieties, and principal components. Am. Stat. 46: 217 — 225.
Google Scholar
Robertson L. D., Abd El Moneim A. M. 1995. Status of Lathyrus germplasm held at ICARDA and its use in breeding programmes. In: R. K. Arora, P. N. Mathur, K. W. Riley & Y. Adham (Eds.), Lathyrus Genetic Resources in Asia. Proceedings of a Regional Workshop, 27–29 December, Raipur, India: 97–111.
Google Scholar
Rybiński W., Bocianowski J. 2006 a. Zmienność cech ilościowych roślin lędźwianu siewnego (Lathyrus sativus L.) pokolenia M1 uzyskanego działaniem chemomutagenów. Biul. IHAR 240/241: 285 — 290.
Google Scholar
Rybiński W., Bocianowski J. 2006 b. Zmienność cech morfologicznych i struktury plonu nasion mutantów lędźwianu siewnego (Lathyrus sativus L.). Biul. IHAR 240/241: 291 — 297.
Google Scholar
Rybiński W., Bocianowski J., Pankiewicz K. 2008 a. Zróżnicowanie cech morfologicznych i plonotwórczych u indukowanych mutantów odmian lędźwianu siewnego (Lathyrus sativus L.). Biul. IHAR 249: 217 — 231.
Google Scholar
Rybiński W., Szot B., Rusinek R. 2008b. Estimation of morphological and mechanical properties of grasspea seeds (Lathyrus sativus L.) originating from EU countries. International Agrophysics 22: 261 — 275.
Google Scholar
Rybiński W., Szot B., Rusinek R., Bocianowski J. 2009. Estimation of geometric and mechanical properties of seed of Polish cultivars and lines representing selected species of pulse crops. International Agrophysics 23: 257—267.
Google Scholar
Sarwar C. D. M., Sarkar A., Murshed A. N. M. M., Malik M. A. 1995. Variation in natural population of grass pea. In: Lathyrus sativus and Human Lathyrism. Progress and Prospects. Proc. 2nd Int. Colloq. Lathyrus Lathyrism, 10–12 December 1993, Dhaka, Bangladesh.
Google Scholar
Sawicka E. 1993. Induced mutations in Andean lupine (Lupinus mutabilis Sweet). Prace Ogrodu Botanicznego. Seria: Monografie i Rozprawy 3: 1 — 101.
Google Scholar
Seidler-Łożykowska K., Król D., Bocianowski J. 2010. Zawartość olejku eterycznego i jego skład w owocach pochodzących z kolekcji kminku zwyczajnego (Carum carvi L.). Rośliny Oleiste — Oilseed Crops XXXI: 147 — 160.
Google Scholar
Vaz Patto M. C., Skiba B., Pang E. C. K., Ochatt S. J., Lambein F., Rubiales D. 2006. Lathyrus improvement for resistance against biotic and abiotic stresses: From classical breeding to marker assisted selection. Euphytica 147: 133 — 147.
Google Scholar
Waghmare V. N., Mehra R. B. 2000. Induced mutations in grasspea (Lathyrus sativus L.). Lathyrus Lathyrism Newsletter 1: 21 — 24.
Google Scholar
Authors
Jan BocianowskiKatedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Katarzyna PankiewiczInstytut Genetyki Roślin PAN w Poznaniu Poland
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