Production of spontaneous and induced doubled-haploid lines of winter triticale obtained through anther culture
Aleksandra Ponitka
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Aurelia Ślusarkiewicz-Jarzina
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Abstract
We investigated the rate of obtaining androgenic plants using anther culture of 15 winter triticale hybrids. Green plants were obtained from all genotypes, with the frequency ranging from 0.4 to 15.2/100 anthers. The medium 190-2, with two different concentrations of kinetin (0.5 and 1.5 mg/l), was used for plant regeneration. Higher number of green plants was observed in the case of the medium with 0.5 mg/l of kinetin (2.5–46.7/ 100 androgenic structures). Ploidy level was determined by flow cytometry in 350 plants and showed from 17.6 to 80.0% spontaneously doubled haploids, depending on the genotype. A total of 80.3% of DH lines was obtained, among which 38.0% were spontaneously doubled haploids, whereas 70.8% of DH lines was obtained from colchicine treatment of 209 haploid plants.
Supporting Agencies
Keywords:
haploid, anther culture, DH lines, winter triticaleReferences
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Authors
Aurelia Ślusarkiewicz-JarzinaInstytut Genetyki Roślin PAN w Poznaniu Poland
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