Estimation of variability on phenotypic and molecular level of spherical-seeded form of grasspea (Lathyrus sativus L.)
Katarzyna Pankiewicz
office@igr.poznan.plInstytut Genetyki Roślin PAN, Poznań (Poland)
Wojciech Rybiński
Instytut Genetyki Roślin PAN, Poznań (Poland)
Zygmunt Kaczmarek
Instytut Genetyki Roślin PAN, Poznań (Poland)
Abstract
A mutant with spherical seeds was investigated together with two Polish cultivars: Derek and Krab (most probable ancestors of the mutant) as well as an Italian grasspee line, typical for South-Western Europe (Mediterranean Basin region). Variation of traits on phenotypic level was estimated basing on results obtained from field trials conducted in four locations in 2008 and 2009. The multivariate analysis method was used. Genetic variation, expressed by degree of genetic similarity, was established with the use of ISSR markers. Moreover, content of protein, fat, fatty acid composition and neurotoxin content (β-ODAP) in seeds was estimated. The obtained results indicated, that the spherical-seeded mutant, as compared to the cultivars Derek and Krab, was significantly different in the following traits: number of branches and pods per plant, pod length, weight of seeds per pod, seeds number per plant, as well as weight of 1000 seeds. Moreover, all three Polish forms represented different morphotype and yield structure parameters than the Italian line. The seeds of spherical-seeded mutant showed higher content of protein and fat as compared to another analyzed forms, with domination of oleic acid in fatty acids composition. All Polish accessions were similar in neurotoxin content in seeds, markedly lower in comparison to the Italian line. Results of hierarchical grouping (after use of the ISSR method) were presented on the dendrogram which indicated that the cultivars and spherical-seeded mutant showed high level of genetic similarity and markedly differed from the Italian line. The results obtained on the phenotypic level from field trials, estimated with use of multivariate statistics, indicated the population from which the cultivar Derek was derived as the most probable ancestor of the spherical seed mutant, however on the molecular level the mutant showed closer genetic similarity to the cultivar Krab.
Keywords:
Lathyrus sativus, chemical composition of seeds, field trials, ISSR markers, spherical-seeded mutant, yield-contributing traitsReferences
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Authors
Wojciech RybińskiInstytut Genetyki Roślin PAN, Poznań Poland
Authors
Zygmunt KaczmarekInstytut Genetyki Roślin PAN, Poznań Poland
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