Identification of the genetic basis of germination in rape seed (Brassica napus L.) using genetic mapping
Katarzyna Gacek
kgacek@nico.ihar.poznan.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0001-8621-8673
Iwona Bartkowiak-Broda
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0002-4071-1849
Laurencja Szala
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0001-9157-7638
Teresa Cegielska-Taras
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0002-6002-9281
Philipp E. Bayer
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0001-8530-3067
David Edwards
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0002-9480-4936
Jacqueline Batley
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0002-5391-5824
Steven Penfield
John Innes Centre, Norwich, UK (United Kingdom)
https://orcid.org/0000-0001-6340-1731
Abstract
The mapping population of winter oilseed rape composed of 78 doubled haploid lines obtained from hybrids originating from the intersection of lines differing in germination power was examined. Seed germination power was automatically recorded every 30 minutes using a camera placed in the Raspberry Pi mini computer. The photos were analyzed using software created at the John Innes Center. In the next step, using the whole genome sequencing method (Illumina® HiSeq) of all mapping population lines, single nucleotide polymorphisms (SNPs) were identified in these lines. This data will be used for genetic mapping and identification of genes regulating the germination process.
Supporting Agencies
The authors would like to acknowledge funding support from the National Science Centre, Poland (Project 2017/01/X/NZ2/00113).
Keywords:
rape seed, genetic mapping, germinationGacek, K. . (2019) “Identification of the genetic basis of germination in rape seed (Brassica napus L.) using genetic mapping ”, Bulletin of Plant Breeding and Acclimatization Institute, (287), pp. 23–24. doi: 10.37317/biul-2019-0100.
Authors
Katarzyna Gacekkgacek@nico.ihar.poznan.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0001-8621-8673
Authors
Iwona Bartkowiak-BrodaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0002-4071-1849
Authors
Laurencja SzalaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0001-9157-7638
Authors
Teresa Cegielska-TarasInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0002-6002-9281
Authors
Philipp E. BayerSchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0001-8530-3067
Authors
David EdwardsSchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0002-9480-4936
Authors
Jacqueline BatleySchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0002-5391-5824
Authors
Steven PenfieldJohn Innes Centre, Norwich, UK United Kingdom
https://orcid.org/0000-0001-6340-1731
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