Genetic similarity of various populations of alfalfa (Medicago sativa L. sl.) and their seed yield potential
Zbigniew Broda
zbroda@up.poznan.plKatedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Agnieszka Dobrzycka
Katedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Jan Bocianowski
Katedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
Several traits affecting seed yield were observed in 16 genetically differentiated populations of alfalfa. The analyzed traits included number of pods per infrutescence, number of seeds per pod, seed yield per infrutescence and fertility. An analysis of genetic similarity was carried out for the populations using RAPD markers, in two series. For the first series the DNA was isolated using the Thomson-Henry’s method and for the other series – with the column method. The dendrograms of genetic similarity varied for both methods and included four and five groups of similarity, respectively. Variability of the observed traits was compared between and within the similarity groups using analysis of variance.
Keywords:
alfalfa, seed yield, RAPD-PCRReferences
Brown P. T. H., Lange F. D., Kranz E., Lorz H. 1993. Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol. Gen. Genet. 235: 157 — 165.
Google Scholar
Crochemore M. L., Huyghe C., Kerlan M. C., Durand F., Julier B. 1996. Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16: 421 — 432.
Google Scholar
Davin-Regli A., Abed Y., Charrel R. N., Bollet C., Micco P. 1995. Variations in DNA concentrations significantly affect the reproducibility of RAPD fingerprint patterns. Res. Microbiol. 146: 561 — 568.
Google Scholar
Delouche J. C. 1980. Environmental effects on seed development and seed quality. Hort. Sc. 15: 775 — 780.
Google Scholar
Echt C. S., Erdahl L. A., McCoy T. J. 1992. Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome 35: 84 — 87.
Google Scholar
Genstat 5 Committee. 1993. Genstat 5 Release 3 Reference Manual. Clarendon Press, Oxford.
Google Scholar
Hu J., Quiros C. F. 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10: 505 — 511.
Google Scholar
Irzykowska L., Bocianowski, J. 2008. Genetic variation, pathogenicity and mycelial growth rate differentiation between Gaeumannomyces graminis var. tritici isolates derived from winter and spring wheat. Annales of Applied Biology 152: 369 — 375.
Google Scholar
Julier B., Porcheron A., Ecalle C., Guy P. 1995. Genetic variability for morphology, growth and forage yield among perennial diploid and tetraploid lucerne populations (Medicago sativa L.). Agronomie 15: 295 — 304.
Google Scholar
Marilla E. F., Scoles G. J. 1996. The use of RAPD markers in Hordeum phylogeny. Genome 39: 646 — 654.
Google Scholar
Meunier J. R., Grimont P. A. D. 1993. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144: 373 — 379.
Google Scholar
Nei M., Li W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269 — 5273.
Google Scholar
Park Y. H., Kohel R. J. 1994. Effects of concentration of MgCl2 on RAPD DNA polymorphism. Biotech. 16: 652.
Google Scholar
Penner G. A., Bush A., Wise R., Kim W., Domier L., Kasha K., Laroche A., Scoles G., Molnar S. J., Fedak G. 1993. Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods Appl. 2: 341 — 345.
Google Scholar
Schachermayr G., Siedler H., Gale M. D., Winzeler H., Winzeler M., Keller B. 1994. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor. Appl. Genet. 88: 110 — 115.
Google Scholar
Słomski R. (red.). 2001. Przykłady analiz DNA — praca zbiorowa: 158 — 160.
Google Scholar
Staszewski Z. 1975. Lucerny. PWRiL, Warszawa.
Google Scholar
Sypniewski J. 1986. Problemy uprawy roślin strączkowych w Polsce. Fragm. Agron. 1: 29 — 36.
Google Scholar
Thomson D., Henry R. 1995. Single step protocol for preparation of plant tissue for analysis by PCR. Biotechniques 19: 394 — 400.
Google Scholar
Volenec J. J., Cunningham S. M., Haagenson D. M., Berg W. K., Joern B. C., Wiersma D. W. 2002. Physiological genetics of alfalfa improvement: past failures, future prospects. Field Crops Research 75: 97 — 110
Google Scholar
Waugh R., Powell W. 1992. Using RAPD markers for crop improvement. Trends Biotech. 10: 186 — 191.
Google Scholar
Weder Jurgen K.P., 2002. Identification of food and feed legumes by RAPD-PCR. Lebensm.-Wiss. und Technol. 35: 504 — 511.
Google Scholar
Willams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531 — 6535.
Google Scholar
Yu K., Pauls K. P. 1993. Rapid estimation of genetic relatedness among heterogeneous populations of alfalfa by random amplification of bulked genomic DNA samples. Theor. Appl. Genet. 86: 788 — 794.
Google Scholar
Authors
Zbigniew Brodazbroda@up.poznan.pl
Katedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Agnieszka DobrzyckaKatedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Jan BocianowskiKatedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu Poland
Statistics
Abstract views: 46PDF downloads: 35
License
Copyright (c) 2009 Zbigniew Broda, Agnieszka Dobrzycka, Jan Bocianowski
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Wojciech Rybiński, Jan Bocianowski, Katarzyna Pankiewicz, Variability of quantitative traits of two mutated grasspea (Lathyrus sativus L.) cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Zbigniew Broda, Danuta Kurasiak-Popowska, Aleksandra Kowalska, Anna Ćwiklińska, The analysis of genetic similarity among Secale species , Bulletin of Plant Breeding and Acclimatization Institute: No. 247 (2008): Regular issue
- Zbigniew Broda, Agnieszka Dobrzycka, The study of self-incompatibility and genetic similarity of genetically differentiated forms of alfalfa (Medicago sativa L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 245 (2007): Regular issue
- Wojciech Rybiński, Michał Starzycki, Elżbieta Starzycka-Korbas, Jan Bocianowski, Genus Lathyrus — estimation and characterization using the chosen species and collected accessions , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Ewa Bakinowska, Jan Bocianowski, Anna Budka, Wiesław Pilarczyk, Bogna Zawieja, Katarzyna Ambroży, Estimation of error variance in unreplicated plant breeding trials with replicated standard treatments , Bulletin of Plant Breeding and Acclimatization Institute: No. 251 (2009): Regular issue
- Agnieszka Tomkowiak, Zbigniew Broda, Krzysztof Moliński, A choice of the mathematical model for the relationship between heterosis effect of F1 hybrids and genetic distance of rye and triticale parental lines , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Jan Bocianowski, Łukasz Stępień, Comparison of five measures of genetic diversity of Polish wheats estimated on the basis of microsatellite markers , Bulletin of Plant Breeding and Acclimatization Institute: No. 242 (2006): Special issue
