Influence of chemical mutagens on morphological traits in petunia (Petunia × atkinsiana D. Don)
Marcelina Krupa-Małkiewicz
kro@zut.edu.plZakład Hodowli Roślin Ogrodniczych, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie (Poland)
Abstract
The objective of the presented study was to induce mutation in petunia ( Petunia atkinsiana D. Don) Flash Red using sodium azide (AS), ethyl methane-sulfonate (EMS), methyl methane-sulfonate (MMS) and diethyl sulphate (DES) of different concentrations 0.5; 1.0; 1.5 and 2.0 mM. Genetic variation of petunia was investigated with the ISSR-PCR method. The morphological changes, observed in mutants in the M1 and M2 generations, referred principally to: lack of pigments in flowers and leaves, darkened midribs, colour of flowers, plant habit (shape of rosette). Frequency of new phenotypes in the progeny populations depended on the mutagen used and its dose. In the M1 generation the most effective were EMS and MMS with doses 1.5 and 2.0 mM, M2 — 2.0 mM MMS proved to be most efficient.
Keywords:
DNA, ISSR-PCR, petunia, mutation, chemical mutagensReferences
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Authors
Marcelina Krupa-Małkiewiczkro@zut.edu.pl
Zakład Hodowli Roślin Ogrodniczych, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie Poland
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