Characteristics of winter oilseed rape (Brassica napus L.) volunteers with the use of RAPD markers
Jan Bocianowski
jan.bocianowski@up.poznan.plKatedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Alina Lieesch
Zakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu (Poland)
Iwona Bartkowiak-Broda
Zakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu (Poland)
Wiesława Popławska
Zakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu (Poland)
Abstract
This study aimed to estimate genetic similarity (GS) and relationships between molecular markers and phenotypic traits and between molecular markers and genotype. The investigations included progenies of 31 volunteers collected from winter oilseed rape fields in the growing season 2005/2006. The plantations were selected in three Voivodships in northern Poland. Winter oilseed rape cultivars Californium, Castille, Lisek, Rasmus, cultivated in the fields of origin of volunteers, and winter turnip rape (B. campestris) Ludowy were chosen as standards. The volunteers of winter oilseed rape have been investigated through 431 RAPD markers. Dendrogram based on Nei and Li (1979) coefficient grouped genotypes in two clusters. The first one was represented by oilseed rape-like plants and standard oilseed rape cultivars; the second group consisted of turnip rape-like plants and turnip rape cultivar Ludowy. Significant associations between molecular markers, phenotypic traits and genotypes were found. Twenty-one of the molecular markers were specific for the turnip rape-like plants with high erucic acid content, and 59 markers were specific for oilseed rape-like plants with low erucic acid content.
Supporting Agencies
Keywords:
molecular marker RAPD, polymorphism DNA, winter oilseed rape (Brassica napus L.), volunteersReferences
Aleksandrzak Ł., Broda Z. 2004. Badanie polimorfizmu „rzepakochwastów” za pomocą markerów RAPD. Rośliny Oleiste – Oilseed Crops XXV (1): 61 — 66.
Google Scholar
Babula D., Kaczmarek M., Barakat A., Delseny M., Quiros C.F., Sadowski J. 2003. Chromosomal mapping of Brassica oleracea based on ESTs from Arabidopsis thaliana: complexity of the comparative map. Mol. Genet. Genomics 268: 656 — 665.
Google Scholar
Barret P., Delourme R., Foisset N., Renard M. 1998. Development of a SCAR (sequence characterised amplified region) marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L. Theor. Appl. Genet. 97: 828 — 833.
Google Scholar
Becker J., Engqvist G.M., Karlsson B. 1995. Comparison of rapeseed cultivars and resynthesized line based on allozyme and RFLP markers. Theor. Appl. Genet. 91: 62 — 67.
Google Scholar
Delourme R., Foisset N., Horvais R., Barret P., Champagne G., Cheung W.Y., Landry B.S., Renard M. 1998. Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.). Theor. Appl. Genet. 97: 129 — 134.
Google Scholar
Delourme R., Pilet-Nayel M.L., Archipiano M., Horvais R., Tanguy X., Rouxel T., Brun H., Renard M., Balesdent M.H. 2004. A cluster of major specific resistance genes to Leptosphaeria maculans in Brassica napus. Phytophatology 94: 578 — 583.
Google Scholar
Doyle J. J., Doyle J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13 — 15.
Google Scholar
Ecke W., Uzunova M., Weißleder K. 1995. Mapping the genome of rapeseed (Brassica napus L.). II. Localization of genes controlling erucic acid synthesis and seed oil content. Theor. Appl. Genet. 91: 972 — 977.
Google Scholar
Foisset N., Delourme R., Barret P., Renard M. 1995. Molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus. Theor. Appl. Genet. 91: 756 — 761.
Google Scholar
Foisset N., Delourme R., Barret P., Hubert N., Landry B.S., Renard M. 1996. Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Theor. Appl. Genet. 93: 1017 — 1025.
Google Scholar
Friedt W., Stoll C., Raclau M., Zarhloul M.K., Lühs W. 2007. Molecular breeding of oilseed rape (Brassica napus) with modified oil quality for nutritional and non-food purposes. Proceedings 12th International Rapeseed Congress, 26-30 March 2007, Wuhan, China, vol. II: 237 — 240.
Google Scholar
Genstat 5 Committee 1993. Genstat 5 Release 3 Reference Manual. Clarendon Press, Oxford.
Google Scholar
Hasan M., Seyis F., Badani A.G., Pons-Kühnemann J. 2006. Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetic Resourses and Crop Evolution 53 (4): 793 — 802.
Google Scholar
Hastie T. J., Tibshirani R. J. 1990. Generalized additive models. Chapman and Hall, London.
Google Scholar
Jain A., Bhatia S., Banga S.S., Prakash S., Lakshmikumaran M. 1994. Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor. Appl. Genet. 88: 116 — 122.
Google Scholar
Javidfar F., Ripley V.L., Roslinsky V., Zeinali H., Abdmishani C. 2006. Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus). Plant Breeding 125: 65 — 71.
Google Scholar
Kole C., Thormann C.E., Karlsson B. H., Palta J. P., Gaffney P., Yandell B., Osborn T. C. 2002. Comparative mapping of loci controlling winter survival and related traits in oilseed Brassica rapa and B. napus. Mol. Breeding 9: 201 — 210.
Google Scholar
Liersch A., Popławska W., Ogrodowczyk M., Bartkowiak-Broda I., Bocianowski J. 2008. Charakterystyka fenotypowa samosiewów rzepaku ozimego (Brassica napus L.) występujących w północnych regionach Polski. Biuletyn IHAR, w druku.
Google Scholar
Liu R., Qian W., Meng J. 2002. Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus × B. campestris). Theor. Appl. Genet. 105: 1050 — 1057.
Google Scholar
Lombard V., Delourme R. 2001. A consensus linkage map for rapeseed (Brassica napus L.): construction and integration of three individual maps from DH populations. Theor. Appl. Genet. 103: 491 — 507.
Google Scholar
Lu G., Cao J., Yu X., Xiang X., Chen H. 2008. Mapping QTLs for root morphological traits in Brassica rapa L. based on AFLP and RAPD markers. J. Appl. Genet. 49(1): 23 — 31.
Google Scholar
Mailer R. J., Scarth R., Fristensky B. 1994. Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers. Theor. Appl. Genet. 87: 697 — 704.
Google Scholar
Mikolajczyk K., Dabert M., Karlowski W., Spasibionek S., Cegielska-Taras K., Bartkowiak-Broda I. 2007. Development of allele-specific SNP markers for the new low-linolenic mutant of winter oilseed rape. Proceedings 12th International Rapeseed Congress, 26–30 March 2007, Wuhan, China, vol. II: 282 — 284.
Google Scholar
Nei M., Li W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269 — 5273.
Google Scholar
Pilet M., Duplan L.G., Archipiano H., Barret P., Baron C., Horvais R., Tanguy X., Lucas M.O., Renard M., Delourme R. 2001. Stability of QTL for field resistance to blackleg across two genetic backgrounds in oilseed rape. Crop. Sci. 41: 197 — 205.
Google Scholar
Rahman M., McVetty P. B. E., Li G. 2007. Inheritance of seed coat color genes of Brassica napus (L.) and tagging the genes using SRAP molecular markers. Proceedings 12th International Rapeseed Congress, 26-30 March 2007, Wuhan, China, vol. II: 310 — 313.
Google Scholar
Santos dos J.B., Nienhuis J., Skroch P., Tivang J., Slocum M. K. 1994. Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes. Theor. Appl. Genet. 87: 909 — 915.
Google Scholar
Snowdon R. J., Friedt W. 2004. Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breeding 123: 1 — 8.
Google Scholar
Sommers D. J., Rakow G., Prabhu V. K., Friesen K. R .D. 2001. Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus. Genome 44: 1077 — 1082.
Google Scholar
Song K.M., Suzuki J.Y., Slocum M.K., Williams P.H., Osborn T.C. 1991. A linkage map of Brassica rapa (syn. campestris) based on restriction fragment length polymorphisms. Theor. Appl. Genet. 82: 296 — 304.
Google Scholar
Uzunova M., Ecke W., Wießleder K. and Röbbelen G. 1995. Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor. Appl. Genet. 90: 194 — 204.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531 — 6535.
Google Scholar
Wu X., Zhang D., Chen B., Lu G., Liu F. 2007. SSR and AFLP fingerprinting of 89 newly bred winter rapeseed cultivars in China. Proceedings 12th International Rapeseed Congress, 26–30 March 2007, Wuhan, China, vol. II: 330 — 332.
Google Scholar
Yu C. Y., Hu S. W., Zhao H. X., Guo A. G., Sun G. L. 2005. Genetic distance revealed by morphological characters, isozymes, proteins and RAPD markers and their relationships with hybrid performance in oilseed rape (Brassica napus L.). Theor. Appl. Genet. 110: 511 — 518.
Google Scholar
Yu F., Lydiate D. J., Hahn K., Kuzmicz S., Hammond Ch., Rimmer S. R. 2007. Identification and mapping of a novel blackleg resistance locus LepR4 in the progenies from Brassica napus × Brassica rapa subsp. sylvestris. Proceedings 12th International Rapeseed Congress, 26-30 March 2007, Wuhan, China, vol. II: 317 — 32.
Google Scholar
Authors
Jan Bocianowskijan.bocianowski@up.poznan.pl
Katedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Alina LieeschZakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu Poland
Authors
Iwona Bartkowiak-BrodaZakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu Poland
Authors
Wiesława PopławskaZakład Genetyki i Hodowli Roślin Oleistych IHAR w Poznaniu Poland
Statistics
Abstract views: 234PDF downloads: 19
License
Copyright (c) 2008 Jan Bocianowski, Alina Lieesch, Iwona Bartkowiak-Broda, Wiesława Popławska
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Marcin Praczyk, Jan Bocianowski, Grażyna Silska, Genetic analysis of fiber content in fibrous flax F1 hybrids , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Jan Bocianowski, Zofia Banaszak, The effect of parental forms on yielding of doubled haploid lines of winter triticale , Bulletin of Plant Breeding and Acclimatization Institute: No. 247 (2008): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Szymon Dziamba, Variation of quantitative traits in Polish local grasspea accessions (Lathyrus sativus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Jan Bocianowski, Waldemar Brukwiński, Analysis of an unreplicated breeding experiment with rye (Secale cereale L.) hybrids , Bulletin of Plant Breeding and Acclimatization Institute: No. 255 (2010): Regular issue
- Sylwia Mikołajczyk, Dorota Weigt, Agnieszka Tomkowiak, Zbigniew Broda, Jan Bocianowski, Haploidization of the rye — the molecular diagnostics and the influence of nanomolecules on supporting the induction and regeneration of plants in in vitro conditions , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Grażyna Mańkowska, Aleksandra Luwańska, Karolina Wielgus, Jan Bocianowski, Evaluation of cannabinoid content in selected varieties of Cannabis sativa L. , Bulletin of Plant Breeding and Acclimatization Institute: No. 277 (2015): Regular issue
- Iwona Bartkowiak-Broda , Alina Liersch, Marcin Matuszczak, Katarzyna Mikołajczyk, Wiesława Popławska, Joanna Wolko, Joanna Nowakowska, The investigations of winter rapeseed genome with the use of molecular markers , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Wojciech Rybiński, Michał Starzycki, Robert Rusinek, Jan Bocianowski, Bogusław Szot, Variation of legume seed’s chemical composition and resistance to mechanical damage , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Alina Lieesch, Wiesława Popławska, Maria Ogrodowczyk, Iwona Bartkowiak-Broda, Jan Bocianowski, Phenotypic characteristics of winter oilseed rape volunteers (Brassica napus L.) occurring in northern regions of Poland , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Michał Starzycki, Analysis of variability for quantitative traits and chemical composition of seeds of chosen accessions of grass pea (Lathyrus sativus L.) and red pea (Lathyrus cicera L.) with different geographical origins , Bulletin of Plant Breeding and Acclimatization Institute: No. 274 (2014): Regular issue
