Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN, Poznań (Poland)
Karolina Krystkowiak
Instytut Genetyki Roślin PAN, Poznań (Poland)
Anetta Kuczyńska
Instytut Genetyki Roślin PAN, Poznań (Poland)
Michał Rębarz
Instytut Genetyki Roślin PAN, Poznań (Poland)
Abstract
The aim of the study was estimation of genetic variability in hull-less barley mutants as compared to their initial form at the phenotypic and molecular levels. Material for the performed studies constituted hull-less barley mutants obtained as a result of mutagenic treatment of grain of hull-less spring barley line — 1N/86. Two chemomutagens were the mutagenic agents. The phenotypic as well as yield structure traits of the mutants were analyzed on the ground of performed field trial. Genetic variations of the mutants, in comparison to their initial line, were elaborated with the use of multivariate statistics methods and the RAPD molecular method. Statistical analysis of the obtained results indicated that the mutants were characterized by broad spectrum of variation, as compared to the initial line. On ground of the molecular study, the calculated genetic similarity coefficients allowed to select the mutants with the greatest and smallest genetic similarity to the initial line. Genetic similarity was estimated according to the formula given by Nei and the results were used for hierarchical grouping of the analyzed mutants. A result of the grouping was presented on the dendrogram. With the use of calculated phenotypic and genetic distances their correlation coefficient was estimated. As final results, the mutants were chosen with the lowest similarity to the initial line, on the both analyzed levels (phenotypic and molecular).
Keywords:
hull-less barley, genetic variation, genetic similarity, mutants, RAPDReferences
Abdel-Fattach, El-Sayed A. A. 2005. Improvement of food hull-less barley in Egypt. International Workshop on Food Barley Improvement, 14–17 January 2002, Hammamet, Tunisia. Proccedings: 7 — 12.
Google Scholar
Akhuary S.B., Sinha A.N., Sinha A. K. 1996. The effectiveness and efficiency of chemical mutagens on biological parameters in hull-less variety of Hordeum vulgare (L.) Karan. Neo Botanica 4 (1): 1 — 5.
Google Scholar
Arumugan S., Reddy V. R. K., Asir R., Viswanathan P., Dhamodaran S. 1997. Induced mutagensis in barley. Advances in Plant Science 10 (1): 103 — 106.
Google Scholar
Barua U. M., Chalmers K. J., Hackett C. A., Thomas W. T. B., Powell W., Waugh R. 1993. Identification of RAPD markers linked to the Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71: 177 — 184.
Google Scholar
Beames R. M., Helm J. H., Eggum B.O., Boisen S., Bach Knudsen K. E., Swift M. L. 1996. A comparison of methods for measuring the nutritive value for pigs of a range of hulled and hulless barley cultivars. Anim. Feed Sci. Technol. 62: 189 — 201.
Google Scholar
Bhatty R. S., Brdhal J. D., Christison G. I. 1975. Chemical composition and digestible energy of barley. Can. J. Anim. Sci., 55: 759 — 764.
Google Scholar
Bhatty R. S. 1986. The potential of hull-less barley — review. Cereal Chem., 63: 97 — 103.
Google Scholar
Bhatty R. S. 1993. Nonmalting uses of barley. In: A. MacGregor and R.S.Bhatty (ed), Barley: Chemistry and Technology. AACC: 355 — 417.
Google Scholar
Bhatty R. S. 1996. Nonwheat grains and products — production of food malt from hull-less barley. Cereal Chemistry 73 (1): 75 — 80.
Google Scholar
Bhatty R.S. 1997. Milling of regular and waxy starch hull-less barleys for the production of bran and flour. Cereal Chemistry 74 (6): 693 — 699.
Google Scholar
Bhatty R. S., Rossnagel B. G. 1998. Comparison of pearled and unpearled Canadian and Japanese barleys. Cereal Chemistry 75 (1): 15 — 21.
Google Scholar
Caliński T., Dyczkowska A., Kaczmarek Z. 1976. Algorytmy Biometrii i Statystyki, Zeszyt 5: 77 — 113.
Google Scholar
Campbell S. G., Mehra R. B., Agraval S.K., Chen Y. Z., Abd El Moneim A.M., Khawaja H .I. T., Yadow C. R., Tay J. U., Araya W. A.. 1994. Current status and future strategy in breeding grasspea (Lathyrus sativus L.). Euphytica 73: 167 — 175.
Google Scholar
Ceranka B., Chudzik H., Czajka S., Kaczmarek Z. 1987. Wielozmienna analiza wariancji dla doświadczeń wieloczynnikowych. Algorytmy Biometrii i Statystyki. Zesz. 6: 51 — 60
Google Scholar
Choo T. W., Ho K. H., Martin R. A. 2001. Genetic analysis of hulless x covered cross of barley using doubled-haploids lines. Crop Sci. 41: 1021 — 1026.
Google Scholar
Czembor H. J. 1989. Stan i perspektywy hodowli w Polsce. Biul. IHAR 171-172: 5 — 14.
Google Scholar
Czembor H. J. 1997. Hodowla jęczmienia w Polsce i Europie. Agrotechnika i Wykorzystanie Jęczmienia, Seminarium Naukowe. Puławy, 23–24 października 1997: 41 — 48.
Google Scholar
Dziamba S., Rachoń L. 1988. Zróżnicowanie elementów struktury plonu nagoziarnistych i oplewionych odmian jęczmienia jarego uprawianych w siewie czystym i mieszanym. Biul. IHAR 167: 79 — 85.
Google Scholar
El-Sayed A. A., El-Enein R. A., El-Gamal A. S. 2004. Two new hull-less barley varieties for rainfed in Egypt. New directions for a diverse plant. 4th International Crop Science Congress, Brisbane, Australia, 26 Sep. – 1 Oct. 2004. Ed. — Fischer T., Proccedings: 28
Google Scholar
Eslick R. F., Blake T., Stallknecht G. 1990. Registration of Wanubet a hulless waxy barley germplasm. Crop Science 30 (6): 1371.
Google Scholar
Górny A. 2004. Zarys Genetyki Zbóż (Zarys Genetyki Jęczmienia), IGR PAN, Poznań. Tom 1: 15 — 142.
Google Scholar
Hang A., Burton C. S., Hoffman D. L., Jones B. L. 2000. Random amplified polymorphic primer-generated embryo DNA polymorphism among 16 North American malting barley cultivars. J. Am. Soc. Brew. Chem. 58 (4): 147 — 151.
Google Scholar
Harlan H. V., Martini M. L., Stevens H. 1940. A study on methods in barley breeding. USDA Washington DC, Technical Bull. 720.
Google Scholar
Helm C. V., De Francisco Gaziola S A., Fornazier R. F., Pompeu G. B., Azevedo R. A. 2004. Hull-less barley varieties: storage proteins and amino acid distribution in relation to nutritional quality. Food Biotechnology 18 (3): 327 — 341.
Google Scholar
Kolasińska K., Boros L. 2003. Wartość siewna oplewionych i nieoplewionych nasion jęczmienia jarego. Hod. Rośl.Nasien. 1/2003: 14 — 15.
Google Scholar
Kroth M. A., Ramella M. S., Tagliari C., De Francisco A., Arisi A. C. M. 2005. Genetic similarity of Brazilian hull-less and malting barley varieties evaluated by RAPD markers. Sci. Agric. 62 (1): 36 — 39.
Google Scholar
Kuczyńska A., Milczarski P., Surma M., Masojć P., Adamski T. 2001. Genetic diversity among cultivars of spring barley revealed by random amplified polymorphic DNA (RAPD). J. Appl. Genet. 42 (1): 43 — 48.
Google Scholar
Kuczyńska A., Bocianowski J., Masojć P., Surma M., Adamski T. 2003. Zastosowanie markerów RAPD do określenia podobieństwa genetycznego odmian jęczmienia ozimego (Hordeum vulgare L.). Biul. IHAR 226/227 (1): 81 — 85.
Google Scholar
Kuczyńska A., Bocianowski J., Surma M., Kaczmarek Z., Adamski T. 2004. Relationship between phenotypic and genetic distances in a set of barley breeding lines. 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June, Proc., 33.
Google Scholar
Lockhart H.N., Hurt D.H. 1986. Nutrition of oats. In: Webster (ed.). Oats: chemistry and technology. St. Paul, AACC: 73 — 130.
Google Scholar
McGuire C.F., Hockett. 1981. Effect of awn length and naked caryopsis of malting quality of Betzes barley. Crop Sci. 21: 18 — 21.
Google Scholar
Nagy J.G. 2003. Economic returns to feed barley yield-increasing and disease resistance research at the Alberta Field Crop Development Centre. Canadian J. of Agricultural Economics 51(30: 281 — 298.
Google Scholar
Nam I.H., Lee E.S. 1990. Effect of waxy hulless genes on endosperm quality, yield and its related traits in barley (Hordeum vulgare L.). Upland and Industrial Crops 32(2): 1 — 12.
Google Scholar
Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction. Proceedings of the Academy of Sciences of the USA 76: 5269 — 5273.
Google Scholar
Noworolnik K., Leszczyńska D. 2004. Reakcja nagoziarnistego jęczmienia jarego na gęstość siewu w porównaniu z jęczmieniem oplewionym. Biul. IHAR 233: 99 — 105.
Google Scholar
Paris R. L. 1999. Development of hulless varieties as an improved feed crop. Barley Newsl., 43: 30.
Google Scholar
Przyborowski J.A., Weeden N.F. 2001. RAPD-based assessment of genetic similarity and distance between Lupinus in section Albus. J. Appl. Genet. 42 (4): 55 — 61.
Google Scholar
Rybiński W. 1981. Short-straw forms of spring barley (Hordeum vulgare L.) obtained as a result of MNUA-induced mutations. Genetica Polonica 22 (3): 271 — 287.
Google Scholar
Rybiński W., Patyna H., Przewoźny T. 1993. Mutagenic effect of laser and chemical mutagens in barley (Hordeum vulgare L.). Genetica Polonica 34 (4): 337 — 343.
Google Scholar
Scholz F. 1965. Experiments on the use of induced mutants to hybridization breeding in barley. Induction of Mutations and the Mutation Process, Praha: 73 — 79.
Google Scholar
Shewry P.R. 1993. Barley seeds proteins. In: MacGregor and Bhatty, Barley: Chemistry and technology. St. Paul, AACC: 1 — 5.
Google Scholar
Takahaski R.H., Inamura H., Matsumoto T. 1961. Effect of genes for covered and naked kernels on some agronomic characters in barley. I. Comparison of two covered and their naked mutants. Ber. Ohara Inst. Landwirtsch. Biol., Okayama Univ. 11: 385 — 392.
Google Scholar
Thair M., Shevtsov V. 1994. New hull-less barley germplasm for high — altitude areas. Rachis 13(1/2): 43 — 46.
Google Scholar
Trop J., Doll H., Haahr V. 1981. Genotypic and environmental influence upon the nutritional composition of barley grain. Euphytica 30 (30; 719 — 728.
Google Scholar
Welsh J., McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213 — 7218.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531 — 6535.
Google Scholar
Vaidya S. M., Mahabalran M. 1989. Karan 3 and Karan 16 — two high yielding hull-less barley varieties. Indian Farming 39 (7): 37 — 38.
Google Scholar
Xue Q., Wang L., Newman R. K., Newman C. W., Graham H. 1997. Influence of hulless, waxy starch and short-awn genes on the composition of barleys. J. of Cereal Sci. 26(2): 251 — 257.
Google Scholar
Yang. W. Z., Beauchemin B. I., Farr B. I., Rode L. M. 1997. Comparison of barley, hull-less barley, and corn in the concentrate of dairy cow. J. Dairy Sci. 80: 2885 — 2895.
Google Scholar
Authors
Karolina KrystkowiakInstytut Genetyki Roślin PAN, Poznań Poland
Authors
Anetta KuczyńskaInstytut Genetyki Roślin PAN, Poznań Poland
Authors
Michał RębarzInstytut Genetyki Roślin PAN, Poznań Poland
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Copyright (c) 2007 Wojciech Rybiński, Karolina Krystkowiak, Anetta Kuczyńska, Michał Rębarz
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