The study of self-incompatibility and genetic similarity of genetically differentiated forms of alfalfa (Medicago sativa L.)
Zbigniew Broda
zbroda@up.poznan.plKatedra Genetyki i Hodowli Roślin, Akademii Rolniczej w Poznaniu (Poland)
Agnieszka Dobrzycka
Katedra Genetyki i Hodowli Roślin, Akademii Rolniczej w Poznaniu (Poland)
Abstract
The study purpose was to analyse genetic similarity between 16 populations of alfalfa using RAPD molecular markers and to describe their self-incompatiblity. The examined objects formed four groups of genetical similarity, which ranged from 42% to 75%. The populations with similar level of self-incompatibility were not in the same group of genetical similarity. To study the self-incompatibility effects, penetration of pollen tubes into the ovaries was observed with the use of fluorescence microscope. According to this, four populations with high self-incompatibility (population Syn 7-3, line F, hybrid F × B10, mutant ‘tf’) and five populations with low self-incompatibility (population Syn 9-3, line B10, hybrid B10 × F, Ulstar and mutant ‘lp’) were distinguished.
Keywords:
alfalfa, self-incompatibility, RAPD markers, genetic similarityReferences
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Authors
Zbigniew Brodazbroda@up.poznan.pl
Katedra Genetyki i Hodowli Roślin, Akademii Rolniczej w Poznaniu Poland
Authors
Agnieszka DobrzyckaKatedra Genetyki i Hodowli Roślin, Akademii Rolniczej w Poznaniu Poland
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Copyright (c) 2007 Zbigniew Broda, Agnieszka Dobrzycka
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