The usefulness of a QTL marker in breeding for apple powdery mildew resistance
Marta Stankiewicz-Kosyl
ino@sggw.edu.plSamodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa (Poland)
Emilian Pitera
Samodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa (Poland)
Stanisław W. Gawroński
Samodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa (Poland)
Abstract
Scab and powdery mildew, caused by Venturia inaequalis (Cke.) Wint. and Podosphaera leucotricha (Ellis et Ev.) Salm. respectively, are the most important apple diseases. The apple clone U 211 is resistant to scab and highly resistant to powdery mildew in field conditions. The QTL U 7, one of the quantitative trait loci in the clone U 211, is responsible for 48–72% of the phenotypic variation, and its effect is stable over years. It was identified in the progeny Idared × U 211. The aim of this study was to assess effectiveness of U 7 marker for marker-assisted selection (MAS). Powdery mildew resistance was evaluated visually in a seedling population Granny Smith U 211 for two years in the nursery, and one year in the orchard using a 0–5 scale. Selection based on the SSR marker NZ28f04 allowed to eliminate 33–72% of plants from class 3 (nursery and orchard), and 100% of plants from class 4 (orchard). The efficiency of selection depended on the year. In the progeny Granny Smith × U 211 neither of the individuals grown in the nursery was classified in class 4 or 5 in the 2- year period of investigations. Twenty-two individuals from the progeny were planted in the orchard. In the following year some of them were classified in class 4. Neither of these plants possessed the NZ28f04 allele, associated with the higher level of resistance to powdery mildew.
Keywords:
apple powdery mildew, microsatellite marker, quantitative trait loci, resistanceReferences
Borecki Z. 1987. Field susceptibility of 13 scab-resistant apple cultivars to apple powdery mildew (Podosphaera leucotricha /Ell. et Ev./ Salm.). Acta Agrobot. 40 (1–2):87 — 98.
DOI: https://doi.org/10.5586/aa.1987.010
Google Scholar
Briard M., Le Clerc V., Grzebelus D., Senalik D., Simon P. W. 2000. Modified protocols for rapid carrot genomic DNA extraction and AFLP analysis using silver staining or radioisotopes. Plant Mol. Biol. Rep. 18: 235 — 241.
DOI: https://doi.org/10.1007/BF02823994
Google Scholar
Calenge F., Faure A., Goerre M., Gebhardt C., van de Weg W. E., Parisi L., Durel C. E. 2004. A QTL analysis reveals both broad-spectrum and isolate-specific QTL for scab resistance in an apple progeny challenged with eight isolates of Venturia inaequalis. Phytopathology 94: 370 — 379.
DOI: https://doi.org/10.1094/PHYTO.2004.94.4.370
Google Scholar
Caranta C., Levebvre V., Palloix A. 1997. Polygenic resistance of pepper to potyviruses consists of a combination of isolate specific and broad spectrum quantitative trait loci. Mol. Plant-Microbe Int. 10: 862 — 870.
DOI: https://doi.org/10.1094/MPMI.1997.10.7.872
Google Scholar
Dalbo M. A., Ye G. N., Weeden W. F., Wilcox W. F., Reisch B. I. 2001. Marker-assisted selection for powdery mildew resistance in grapes. J. Amer. Soc. Hort. Sci. 126: 83 — 89.
DOI: https://doi.org/10.21273/JASHS.126.1.83
Google Scholar
Fasoula V. A., Harris D. K., Boerma H. R. 2004. Validation and designation of quantitative trait loci for seed protein, seed oil and seed weight from two soybean populations. Crop Sci. 44: 1218 — 1225.
DOI: https://doi.org/10.2135/cropsci2004.1218
Google Scholar
Gadamski G., Ciarka D., Gawronski S. W. 1996. Molecular survey of Polish resistant biotypes of weeds. Proc. Sec. Int. Weed Control Congress. Copenhagen: 547 — 550.
Google Scholar
Guilford P., Prakash S., Zhu J. M., Rikkerink E., Gardiner S., Bassett H., Forster R. 1997. Microsatellites in Malus domestica (apple) abundance, polymorphism and cultivar identification. Theor. Appl. Genet. 94: 249 — 254.
DOI: https://doi.org/10.1007/s001220050407
Google Scholar
Kolb F. L., Bai G. H., Muehlbauer G. J., Anderson J. A., Smith K. P., Fedak G. 2001. Host plant resistance genes for Fusarium head blight: mapping and manipulation with molecular markers. Crop Sci. 41: 611 — 619.
DOI: https://doi.org/10.2135/cropsci2001.413611x
Google Scholar
Krieghoff O. 1995. Entwicklung einer In-vitro-Selectionsmethode auf Resistenz von Malus-Genotypen gegenuber Podosphaera leucotricha (Ell. et Ev.) Salm. und In-vitro-Differenzierung von Virulenzunterschieden des Erregers. Ph D dissertation, Humboldt-Universitat, Berlin.
Google Scholar
Pitera E. 2003. Przydatność klonu U 211 w hodowli odpornościowej jabłoni. Rozprawy Naukowe i Monografie. Wyd. SGGW-AR Warszawa.
Google Scholar
Rogers S. O., Bendich A. J. 1985. Extraction of DNA from miligram amounts of fresh, herbarium and mummified plant tissues. Plant. Mol. Biol. 5: 69 — 76.
DOI: https://doi.org/10.1007/BF00020088
Google Scholar
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Google Scholar
Sewell M. M., Bassoni D. L., Megraw R. A., Wheeler N. C., Neale D. B. 2000. Identification of QTLs influencing wood property traits in loblolly pine (Pinus taeda L.). I. Physical wood properties. Theor. Appl. Genet. 101: 1273 — 1281.
DOI: https://doi.org/10.1007/s001220051607
Google Scholar
Stankiewicz-Kosyl M., Pitera E., Gawronski S.W. 2005. Mapping QTL involved in powdery mildew resistance of the apple clone U 211. Plant Breed. 124: 63 — 66.
DOI: https://doi.org/10.1111/j.1439-0523.2004.01049.x
Google Scholar
Viviani A., Kellerhals M., Gianfranceschi L., Gessler C. 1996. Towards marker-assisted breeding of scab resistant apple cultivars. Eucarpia Fruit Breeding Section Newsletter 2: 25 — 26.
Google Scholar
Zhou W. C., Kolb F. L., Bai G. H., Domier L. L., Boze L. K., Smith N. J. 2003. Validation of major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breed. 122: 40 — 46.
DOI: https://doi.org/10.1046/j.1439-0523.2003.00802.x
Google Scholar
Authors
Marta Stankiewicz-Kosylino@sggw.edu.pl
Samodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa Poland
Authors
Emilian PiteraSamodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa Poland
Authors
Stanisław W. GawrońskiSamodzielny Zakład Przyrodniczych Podstaw Ogrodnictwa, Wydział Ogrodnictwa i Architektury Krajobrazu, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa Poland
Statistics
Abstract views: 9PDF downloads: 2
License
Copyright (c) 2006 Marta Stankiewicz-Kosyl, Emilian Pitera, Stanisław W. Gawroński
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
