Effect of pH and nitrogen content in substrate on the yield of Withania somnifera (L.) Dun.
Grażyna Obidowska
grazyna_obidowska@sggw.plKatedra Genetyki, Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa (Poland)
Ala Sadowska
Katedra Genetyki, Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa (Poland)
Abstract
Withania somnifera, a subtropical plant from Solanaceae family, is a very interesting medicinal species. In the regions of its natural occurrence, especially in India, the root of Withania (Radix Withaniae) has been widely used as an energizing, immunostimulating and adaptogenic drug. The aim of experiments carried out in the greenhouses of Agricultural University in Warsaw in 1997–1999, was to verify the hypothesis that: the observed in Withania lack of response to nitrogen fertilization is not a result of low demand for nitrogen, but it is caused by the pH of soils in which the plant has been grown in India. The experimental plants were grown in peat substrate with pH adjusted to 5, 6 and 7. Nitrogen contents were: 100, 200, 300, 350 and 400 mg/dm3, while the constant phosphorus and potassium levels were set to 80 and 300 mg/dm3, respectively. The results suggest that Withania somnifera prefers acidic soils. The highest yields of herb and roots, as well as withaferine A content and yield, were obtained at pH 5. The plants took up significantly more minerals (nitrogen, potassium and phosphorus) than the ones grown at a higher pH of soil. Withania somnifera seems to have high demand for nitrogen, and the observed lack of reaction to nitrogen fertilization is probably due to inappropriate pH of soils, on which it is grown in India.
Keywords:
Withania somnifera, nitrogen, pH, withaferine AReferences
Bhattacharya A., Ghosal S., Bhattacharya S. K. 2001. Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum. J. Ethnopharmacol., 74 (1): 1 — 6.
DOI: https://doi.org/10.1016/S0378-8741(00)00309-3
Google Scholar
Bhattacharya A., Ramanathan M., Ghosal S., Bhattacharya S. K. 2000 a. Effect of Withania somnifera glycowithanolides on iron-induced hepatotoxicity in rats. Phytotherap. Res., 14 (7): 568 — 570.
DOI: https://doi.org/10.1002/1099-1573(200011)14:7<568::AID-PTR663>3.0.CO;2-Q
Google Scholar
Bhattacharya S. K., Bhattacharya A., Sairam K., Ghosal S. 2000 b. Anxiolytic-antidepressant activity of Withania somnifera glycowithanolides: an experimental study. Phytomed., 7 (6): 463 — 469.
DOI: https://doi.org/10.1016/S0944-7113(00)80030-6
Google Scholar
Dahatoude B. N., Joshi B. G., Vitkare D. G. 1983. Studies on response of nitrogen fertilization on the root yield of ashwagandha (Withania somnifera). Punj. Krish. Vid. Res. J., 7 (1): 7 — 8.
Google Scholar
Demeyer K., Dejaegere R. 1997. Nitrogen and alkaloid accumulation and partitioning in Datura stramonium L. J. Herb. Spices Med. Plant., 5 (3): 15 — 23.
DOI: https://doi.org/10.1300/J044v05n03_03
Google Scholar
Golcz L., Kordana S., Nowak S. 1977. Potrzeby i dynamika pobierania składników pokarmowych przez pokrzyk wilczą jagodę (Atropa belladonna L.). Herba Pol. 23: 247 — 261.
Google Scholar
Golcz L., Kordana S., Załęcki R. 1970. Potrzeby pokarmowe pieprzowca rocznego (Capsicum annuum L.). Herba Pol., 16: 107 — 125.
Google Scholar
Gomez I., Navarro-Pedreno J., Moral R., Iborra M. R., Palacios G., Mataix J. 1996. Salinity and nitrogen fertilization affecting the macronutrient content and yield of sweet pepper plants. J. Plant. Nutr., 19 (2): 353 — 359.
DOI: https://doi.org/10.1080/01904169609365126
Google Scholar
Hegde D. M. 1997. Nutrient requirements of solanaceous vegetable crops. Ext. Bull. ASPAC Food Fert. Tech., 441: 9.
Google Scholar
Łata B. 1993. Wpływ nawożenia azotem oraz dolistnego dokarmiania azotem, fosforem, potasem i cynkiem na plon surowca i zawartość alkaloidów u barwinka różowego [Catharanthus roseus (L.) G. Don]. Praca doktorska, SGGW, Warszawa.
Google Scholar
Maitra S., Jana B. K., Debnath S. 1998. Response of plant nutrients on growth and alkaloid content of Ashwagandha (Withania somnifera Dunal). J. Interacadem., 2 (4): 243 — 246.
Google Scholar
Mengel K., Kirkby E. A. 1983. Podstawy żywienia roślin. PWRiL, Warszawa.
Google Scholar
Mulonas B. A., Stergianopoulos G. N., Maurommates A. Z. 1980. Effect of nitrogen and potassium on yield and chemical composition of Basma tabacco. Georg. Erouna, 4: 229 — 239.
Google Scholar
Nigam K. B., Rawat G. S., Prasad B. 1984. Effect of methods of sowing, plant density and fertility level on aswagandha (Withania somnifera Dunal). South Indian Hort. 32 (6): 356 — 359.
Google Scholar
Nowacki E. 1980. Gospodarka azotowa roślin uprawnych. PWRiL, Warszawa.
Google Scholar
Nowotny-Mieczyńska A. 1976. Fizjologia mineralnego żywienia roślin. PWRiL, Warszawa.
Google Scholar
Obidoska G. 2001. Wpływ czynników agrotechnicznych na plon surowca i nasion Withania somnifera (L.) Dun. uprawianej w warunkach glebowo-klimatycznych Polski. Praca doktorska, SGGW, Warszawa.
Google Scholar
Ozguven M., Sener B. 1992. Drug yield and withanolide contents of Withania somnifera (L.) Dunal cultivated in Turkey. Acta Hort., 306: 219 — 227.
DOI: https://doi.org/10.17660/ActaHortic.1992.306.24
Google Scholar
Piskornik Z. 1994. Fizjologia roślin dla wydziałów ogrodniczych. Część I. AR im. H. Kołłątaja, Kraków.
Google Scholar
Roja G., Heble M. R., Sipahimdani A. T. 1991. Tissue cultures of Withania somnifera, morphogenesis of withanolide synthesis. Phytotherap. Res., 5 (4): 185 — 187
DOI: https://doi.org/10.1002/ptr.2650050411
Google Scholar
Russo A., Izzo A. A., Cardile V., Borrelli F., Vanella A. 2001. Indian medicinal plants as antiradicals and DNA cleavage protectors. Phytomed., 8 (2): 125 — 132.
DOI: https://doi.org/10.1078/0944-7113-00021
Google Scholar
Shohat B., Kirson I., Lavie D. 1978. Immunosuppressive activity of two plant steroidal lactones, withaferin A and withanolide E. Biomedicine, 28 (1): 18 — 24.
Google Scholar
Wagner H., Norr H., Winterhoff H. 1994. Plant Adaptogens. Phytomed. 1 (1): 63 — 76.
DOI: https://doi.org/10.1016/S0944-7113(11)80025-5
Google Scholar
Authors
Grażyna Obidowskagrazyna_obidowska@sggw.pl
Katedra Genetyki, Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa Poland
Authors
Ala SadowskaKatedra Genetyki, Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa Poland
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