Induction of haploids in rye (Secale cereale L.)
Zbigniew Broda
agro@up.poznan.plKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza, Poznań (Poland)
Sylwia Mikołajczyk
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza, Poznań (Poland)
Abstract
Inbred lines production in rye (Secale cereale L.) with the classical breeding procedure is a difficult and time consuming task. With use of in vitro culture techniques for obtaining doubled haploid plants of rye it can be done in a shorter time. In cereals, for the haploid induction the androgenesis (anther culture and isolation microspores) and elimination of chromosomes are widely used. The most important factors influencing the androgenic capacity are: growth of donor plant material, genotype, the initial treatment of spikes and anthers, the stage of microspores development, the medium and culture condition. Rye (Secale cereale L.) is a species in which induction of haploids in vitro anther cultures causes many problems. The most frequent occurring problems are low percentage of regenerated plants and high participation albinotic plants.
Keywords:
Secale cereale, androgenesis, factors, elimination of chromosomes, haploids, ryeReferences
Bajaj Y. P. S. 1990. Biotechnology in agriculture and forestry: haploids in crop improvement I. Springer-Verlag, Berlin, Vol. 12: 18/20: 125 — 127.
DOI: https://doi.org/10.1007/978-3-642-61499-6
Google Scholar
Broda Z., Mikołajczyk S. 2001. Próby indukowania haploidów żyta (Secale cereale) metodą kultur pylnikowych. Biotechnologia, 1/52: 148 — 152.
Google Scholar
Daniel G., 1993. Anther culture in rye: improved plant regeneration using modified MS-media. Plant Breeding, 110: 259 — 261.
DOI: https://doi.org/10.1111/j.1439-0523.1993.tb00587.x
Google Scholar
Deimling S., Flehinghaus-Roux T. 1997. Haploidy in rye. In: In vitro haploid production in higher plants. S. M. Jain, S. K. Sopory, Veilleux R. E. (eds.). Kluwer Academic Publishers, Dordrecht: Vol. 4: 181 — 204.
DOI: https://doi.org/10.1007/978-94-017-1862-2_10
Google Scholar
Deimling S., Geiger H. H. 1996. Anther culture in rye: Methodical improvements and genetic analysis. Vortr. Pflanzenzüchtg.: 35: 225 — 235.
Google Scholar
Deimling S., Flehinghaus-Roux T., Röber F., Schechert S., Roux R., Geiger H. H. 1994. Doubled haploid production — now reproducible in rye. In: Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, June 12–17, 1994: 95.
Google Scholar
Flehinghaus T., Deimling S., Geiger H. H. 1995. Anther-culture ability in Secale cereale L. Plant Breeding 114: 259 — 261.
DOI: https://doi.org/10.1111/j.1439-0523.1995.tb00807.x
Google Scholar
Flehinghaus-Roux T., Deimling S., Geiger H. H. 1991. Methodical improvements in rye anther culture. Plant Cell Reports 10: 397 — 400.
DOI: https://doi.org/10.1007/BF00232610
Google Scholar
Hörlein A. J. 1991. Methodische Untersuchungen zur Antherenkultur bei Roggen. Ph. D. Thesis, University of Hohenheim, Stuttgart.
Google Scholar
Immonen S., Anttila H. 1999. Cold pretreatment to enhance green plant regeneration from rye anther culture. Plant Cell, Tissue and Organ Culture 57: 121 — 127.
DOI: https://doi.org/10.1023/A:1006381516632
Google Scholar
Immonen S. 1999. Androgenetic green plants from winter rye, Secale cereale L., of diverse origin. Plant Breeding 118: 319 — 322.
DOI: https://doi.org/10.1046/j.1439-0523.1999.00381.x
Google Scholar
Immonen S., Anttila H. 1996. Success in anther culture of rye. Vortr. Pflanzenzüchtg. 35: 237 — 244.
Google Scholar
Kasha K. J., Kao K. N. 1970. High frequency haploid production in barley (Hordeum vulgare L.). Nature 225: 874 — 876.
DOI: https://doi.org/10.1038/225874a0
Google Scholar
Larsen E. T., Tuvesson I. K. D., Andersen S. B. 1991. Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture. Theor. Appl. Genet. 82: 417 — 420.
DOI: https://doi.org/10.1007/BF00588593
Google Scholar
Laurie D. A., Bennett M. D. 1988. The production of haploid wheat plants from wheat × maize crosses. Theor. Appl. Genet. 76: 393 — 397.
DOI: https://doi.org/10.1007/BF00265339
Google Scholar
Marsolais A. A., Seguin-Swartz G., Kasha K. J. 1984. The influence of anther cold pretreatments and donor plant genotypes on in vitro androgenesis in wheat (Triticum aestivum L.). Plant Cell, Tissue and Organ Culture 3: 69 — 79.
DOI: https://doi.org/10.1007/BF00035922
Google Scholar
Milewska-Pawliczuk E. 1987. Induction of androgenesis in vitro in various genetic forms of Secale cereale. Biologia Plantarum 29/4: 295 — 298.
DOI: https://doi.org/10.1007/BF02892793
Google Scholar
Orlikowska T. 1977. Induction of androgenesis in vitro in Secale cereale and Triticale. Genet. Pol. 18/1: 51 — 58.
Google Scholar
Powell W. 1988. The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgare L.). Plant Cell, Tissue and Organ Culture 12: 291 — 297.
DOI: https://doi.org/10.1007/BF00034371
Google Scholar
Rakoczy-Trojanowska M., Kwaśniak A., Malepszy S. 1996. Factors influencing viability and sporophytic development in vitro of rye (Secale cereale L.) microspores. Vortr. Pflanzenzüchtg. 35: 256 — 257.
Google Scholar
Rakoczy-Trojanowska M., Śmiech M., Malepszy S. 1996 a. An evaluation of the factors influencing anther culture of rye (Secale cereale L.) Vortr. Pflanzenzüchtg. 35: 260 — 261.
Google Scholar
Rakoczy-Trojanowska M., Śmiech M., Malepszy S. 1997. The influence of genotype and medium on rye (Secale cereale L.) anther culture. Plant Cell, Tissue and Organ Culture 48: 15 — 21.
DOI: https://doi.org/10.1023/A:1005792912316
Google Scholar
Sharma G. C., Wen Chung Wang, Sapra V. T. 1982. Effect of genotype, media and temperature pretreatment on calus initiation in triticale, wheat, and rye anther cultures. Cereal Res. Commun. 10 3/4: 143 — 150.
Google Scholar
Suenaga K. 1994. Doubled haploid system using the intergeneric crosses between wheat (Triticum aestivum) and maize (Zea mays). Bull. Natl. Inst. Agrobiol. Resour. 9: 83 — 139.
Google Scholar
Szarejko I. 1994. Techniki otrzymywania podwojonych haploidów i ich wykorzystanie w hodowli roślin. Prace Ogrodu Botanicznego PAN 5/6: 39 — 53.
Google Scholar
Ślusarkiewicz-Jarzina A., Ponitka A., Woźna J. 1999. Kultury pylnikowe, izolowane mikrospory i oddalone krzyżowanie w uzyskiwaniu haploidów żyta. I Krajowa Konferencja „Haploidy i linie podwojonych haploidów w genetyce i hodowli roślin”. Instytut Genetyki Roślin PAN, Poznań: 30.
Google Scholar
Thomas E., Hoffmann F., Wenzel G. 1975. Haploid plantlets from microspores of rye. Z. Pflanzenzüchtg. 75: 106 — 113.
Google Scholar
Tuvesson I. K. D., Pedersen S., Andersen S. B. 1989. Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture. Theor. Appl. Genet. 78: 879 — 883.
DOI: https://doi.org/10.1007/BF00266675
Google Scholar
Wenzel G., Foroughi-Wehr B. 1990. Haploidenkultur. Agrarspectrum 17: 85 — 98.
Google Scholar
Wenzel G., Hoffmann F., Thomas E. 1976. Heterozygous microspore-derived plants in rye. Theor. Appl. Genet. 48: 205 — 208.
DOI: https://doi.org/10.1007/BF00527372
Google Scholar
Wenzel G., Hoffmann F., Thomas E. 1977. Increased induction and chromosome doubling of androgenetic haploid rye. Theor. Appl. Genet. 51: 81 — 86.
DOI: https://doi.org/10.1007/BF00299481
Google Scholar
Wenzel G, Thomas E. 1974. Observations on the growth in culture of anthers of Secale cereale. Z. Pflanzenzüchtg. 72: 89 — 94.
Google Scholar
Wędzony M., Marcińska I., Ponitka A., Ślusarkiewicz-Jarzina A., Woźna A. 1998. Production of doubled haploids in triticale (X Triticosecale Wittm.) by means of crosses with maize (Zea mays L.) using picloram and dicamba. Plant Breeding 117: 211 — 215.
DOI: https://doi.org/10.1111/j.1439-0523.1998.tb01928.x
Google Scholar
Authors
Zbigniew Brodaagro@up.poznan.pl
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza, Poznań Poland
Authors
Sylwia MikołajczykKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza, Poznań Poland
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