The analysis of genetic diversity of rye inbred lines and their uniformity
Iwona Wiśniewska
i.wisniewska@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Andrzej Rafalski
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Lucjan Madej
Zakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The usefulness of PCR systems for detection of genetic diversity among inbred lines of rye (Secale cereale L) and for evaluation of homogeneity of two inbreds was investigated. In comparison to RAPD primers, the primers targeting the sequences of intron-exon junction generated more complex and polymorphic patterns of amplified DNA fragments. Some primers 18 bases in length generated too complex patterns of DNA fragments, which may cause the difficulties in their computer analysis. Clear and polymorphic banding patterns were generated with the use of primers 12–15 bases in length. The evaluation of genetic differences between single plants of the lines indicated that this approach is useful for assessment of homogenity level of varieties or inbred lines.
Keywords:
PCR, genetic distance, inbred lines, ryeReferences
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Authors
Iwona Wiśniewskai.wisniewska@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Andrzej RafalskiZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Lucjan MadejZakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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