A simple method of selecting wheat and triticale breeding materials using a crude filtrate containing the Tox3 effector
Jakub Walczewski
j.walczewski@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin – Państwowy Instytut Badawczy, Radzików, 05‒870 Błonie (Poland)
https://orcid.org/0000-0002-9045-1299
Abstract
Parastagonospora nodorum is a wide spread necrotrophic pathogen of crop that primary attack wheat and triticale. It is casual agent of septoria nodorum leaf and glume blotch (SNB), which in favorable weather conditions causes large yield losses. This pathogen uses a number of specific protein effectors that in sensitive genotypes trigger signaling pathways leading to programmed cell death, resulting in necrotic changes in infected tissue. This work presents the procedure for testing breeding objects for sensitivity to the Tox3 effector using non-purified filtrate from P. nodorum culture. This approach allows achieving satisfactory selection results without the need for troublesome purification procedures or expression based on genetically modified microorganisms.
Keywords:
effectors, SNB, nodorum, necrotroph, selection, resistance breedingReferences
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Authors
Jakub Walczewskij.walczewski@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin – Państwowy Instytut Badawczy, Radzików, 05‒870 Błonie Poland
https://orcid.org/0000-0002-9045-1299
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