Pyramiding of resistance genes against Phytophthora infestans in potato
Jarosław Plich
j.plich@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Beata Tatarowska
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Dorota Milczarek
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Bogdan Flis
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Abstract
Pyramiding of the resistance genes against P. infestans enhances effectiveness and durability of resistance used in potato breeding programs. The aim of presented study was to combine two broad-spectrum resistance genes against P. infestans. The resistance donors cultivar Bzura (possessing R2-like gene) and potato clone 04-IX-21 (possessing Rpi-phu1 gene) were crossed, and unselected progeny was developed. The resistance of progeny clones was tested in three consecutive years in detached leaflet tests with the use of three groups of isolates different in terms of virulence profiles. Based on the results of phenotypical tests, a group of progeny clones combining both resistance genes were selected. Since phenotypical tests are very laborious and time-consuming, authors proposed selection of such clones with the use of DNA markers specific for these genes. Results of genotyping were fully consistent with phenotypic tests, which confirms that proposed DNA markers can be used for selection of resistant clones in breeding programs.
Keywords:
gene Rpi-phu1, gene R2-like, late blight, R genesReferences
Brylińska M., Sobkowiak S., Stefańczyk E., Śliwka J. 2016. Potato cultivation system affects population structure of Phytophthora infestans. Fungal Ecology 20: 132 — 143.
Google Scholar
Cooke L. R., Schepers H. T. A. M., Hermansen A., Bain R. A., Bradshaw N. J., Ritchie F., Shaw D. S., Evenhuis A., Kessel G. J. T., Wander J. G. N., Andersson B., Hansen J.G., Hannukkala A., Nærstad R., Nielsen B. J. 2011. Epidemiology and integrated control of potato late blight in Europe. Potato Res. 54: 183 — 222.
Google Scholar
DeFauw S. L., He Z., Larkin R. P., Mansour S. A. 2012. Sustainable potato production and global food security. W: Sustainable Potato Production: Global Case Studies. He Z. Larkin R., Honeycutt W. Springer Science + Business Media B.V. 2012.
Google Scholar
Forbes G. A. 2012. Using host resistance to manage potato late blight with particular reference to developing countries. Potato Res. 55: 205 — 216.
Google Scholar
Gilroy E. M., Breen S., Whisson S. C., Squires J., Hein I., Kaczmarek M., Turnbull D., Boevink P. C., Lokossou A., Cano L. M., Morales J., Avrova A. O., Pritchard L., Randall E., Lees A., Govers F., van West P., Kamoun S, Vleeshouwers V. G. A. A., Cooke D. E .L., Birch P. R. J. 2011. Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2 — like in Phytophthora infestans determine virulence on R2 plants. New Phytologist 191: 763 — 776.
Google Scholar
Kim H. J., Lee H. R., Jo K. R., Mahdi Mortazavian S. M., Huigen D.J., Evenhuis B., Kessel G., Visser R. G. F., Jacobsen E., Vossen J. H. 2012. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theor. Appl. Genet. 124: 923 — 935.
Google Scholar
Plich J., Tatarowska B., Milczarek D., Zimnoch-Guzowska E. Flis B. 2016. Relationships between race-specific and race-non-specific resistance to potato late blight and length of potato vegetation period in various sources of resistance. Field Crops Research 196: 311 — 324.
Google Scholar
Plich J., Tatarowska B., Lebecka R. Śliwka J., Zimnoch-Guzowska E., Flis B. 2015. R2-like Gene Contributes to Resistance to Phytophthora infestans in Polish Potato Cultivar Bzura. Am. J. Potato Res. 92 (3): 350 — 358.
Google Scholar
Śliwka J. Jakuczun H., Lebecka R., Marczewski W., Gebhardt C., Zimnoch-Guzowska E. 2006. The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period. Theor. Appl. Genet. 113: 685 — 695.
Google Scholar
Śliwka J., Świątek M., Tomczyńska I., Stefańczyk E., Chmielarz M., Zimnoch-Guzowska E. 2013. Influence of genetic background and plant age on expression of the potato late blight resistance gene Rpi-phu1 during incompatible interaction with Phytophthora infestans. Plant Pathology 62: 1072 — 1080.
Google Scholar
Vleeshouwers V. G. A. A., Raffaele S., Vossen J., Champouret N., Oliva R., Segretin M. E., Rietman H., Cano L. M., Lokossou A., Kessel G., Pel M. A., Kamoun S. 2011. Understanding and exploiting late blight resistance in the age of effectors. Annual Review of Phytopathology 49: 25.1 — 25.25.
Google Scholar
Zarzycka H. 2001. Ocena odporności na zarazę ziemniaka w teście listkowym. Sporządzanie inokulum. Instytut Hodowli i Aklimatyzacji Roślin, Radzików, Monografie i Rozprawy Naukowe IHAR 10/2001: 77 — 80.
Google Scholar
Authors
Jarosław Plichj.plich@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Beata TatarowskaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Dorota MilczarekInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Bogdan FlisInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
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