Assessment of growth and genetic stability of gooseberry (Ribes grossularia L.)propagated in vitro and ex vitro
Danuta Kucharska
danuta.kucharska@inhort.plInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0002-9635-967X
Danuta Wójcik
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
https://orcid.org/0000-0002-3556-8080
Aleksandra Trzewik
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
https://orcid.org/0000-0003-0378-5440
Abstract
The aim of the study was to evaluate phenotypically and genetically, 15 gooseberry genotypes plants propagated in vitro and ex vitro by cuttings grown in the second year in field conditions. The outcome of this was that in vitro propagated shrubs were shown to grew more strongly. Their height and the number of shoots were significantly higher for the 11 genotypes and a width of 12 genotypes for gooseberry. However, fruit abundance was greater in much more traditionally multiplied plants. Beyond the aforementioned, Genetic stability analysis of clones derived from in vitro cultures of five gooseberry varieties was performed. Herein, 13-15 plants with in vitro and mother plants were analyzed. The number of products generated by the AFLP primer pairs ranged from 33 to 108. The highest total number of amplification products was obtained as a result of the AFLP reaction for the plants ‚Hinnonmaki Rot’ (300), and the lowest for the varieties ‚Hinsel’ and ‚Resika’ (262). Genetic variability in gooseberry in vitro plants ranged from 1.03% for ‚Captivator’, to 10.3% for ‚Hinsel’. Genetic stability was also assessed using ISSR markers. Plants of five genotypes derived from conventional and in vitro reproduction were used. Accordingly, a total of 2294 amplification products were obtained, of which 2.8% were polymorphic. The size of the obtained products was from 250 to 2900 bp, depending on the starter and variety. ISSR-PCR analysis showed different degrees of polymorphism - from 0 for the ‘Hinnonmaki Rot’ and ‘Resica’ to 11.6% for the ‚Hinsel’ variety.
Supporting Agencies
Keywords:
AFLP, gooseberry, DNA, ISSR, in vitro cultures, polymorphismReferences
Bahulikar R.A., Stanculescu D., Preston C.A., Baldwin I.T. 2004. ISSR and AFLP analysis of the temporal and spatial population structure of the post-fire annual, Nicotiana attenuata, in SW Utah. BMC Ecol. 4: 12.
Google Scholar
Cavanna M., Marinoni D.T., Beccaro G.L., Bounous G. 2009. Microsatellite-based evaluation of Ribes spp. germplasm. Genome 52(10): 839-848.
Google Scholar
Costa R., Pereira G., Garrido I., Tavares-deSousa M.M., Espinosa F. 2016. Comparison of RAPD, ISSR, and AFLP molecular markers to reveal and classify orchardgrass (Dactylis glomerata L.) germplasm variations. PLoS ONE 11(4): e0152972. doi:10.1371/journal. pone.0152972.
Google Scholar
Drew R.A., Smith M.K. (1990): Field evaluation of tissuecultured bananas in south-eastern Queensland. Austr. J. Exp. Agric., 30: 569–574.
Google Scholar
Dubois L.A.M., Roggermans J., Soyeurt G., de Vries D.P. (1988): Comparison of the growth and development of dwarf rose cultivars propagated in vitro and in vivo by softwood cuttings. Sci. Hortic., 55: 293–299.
Google Scholar
Howard B.H., Jones O.P., Vasek J. (1989): Long-term improvement in the rooting of plum cuttings following apparent rejuvenation. J. Hort. Sci., 64: 147–156.
Google Scholar
Korbin M., Kuras A., Żurawicz E. 2002. Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR. Cell. Mol. Biol. Lett. 7(2B): 785-94.
Google Scholar
Kucharska D., Orlikowska T., Maciorowski R., Kunka M., Wójcik D., Pluta S. 2020. Application of meta-topolin for improving micropropagation of gooseberry (Ribes grossularia). Sci. Hort., 272: 109529. https://doi. org/10.1016/j.scienta.2020.109529
Google Scholar
Kumar A., Misra P., Dube A. 2013. Amplified fragment length polymorphism: an adept technique for genome mapping, genetic differentiation, and intraspecific variation in protozoan parasites. Parasitol. Res. 112: 457-466.
Google Scholar
Lanham P.G., Brennan R.M. 1999a. Genetic characterization of gooseberry (Ribes grossularia subgenus Grossularia) germplasm using RAPD, ISSR and AFLP markers. J. Hort. Sci. Biotech. 74(3): 361-366.
Google Scholar
Lanham P.G., Brennan R.M. 1999b. Genetic characterisation of Ribes nigrum, Ribes rubrum and Ribes grossularia. Acta Hort. 505: 385-392.
Google Scholar
Lanham P.G., Brennan R.M. 2001. Genetic diversity in Ribes. Acta Hort. 546: 135-137.
Google Scholar
Meudt H.M., Clarke A.C. 2007. Almost forgotten or latest practice? AFLP applications, analyses and advances. Trends Plant Sci. 12(3): 106–117.
Google Scholar
Podwyszyńska M., Niedoba K., Korbin M., Marasek A. 2006. Somaclonal variation in micropropagated tulips determined by phenotype and DNA markers. Acta Hort. 714: 211-220.
Google Scholar
Podwyszyńska M., Pluta S. 2019. In vitro tetraploid induction of the blackcurrant (Ribes nigrum L.) and preliminary phenotypic observations. Zemdirbyste-Agriculture, 106(2), 151-158.
Google Scholar
Reed B.M., Hummer K.E. 2002. Cryopreservation of Ribes. W: Towill L.E., Bajaj Y.P.S. (red.), Cryopreservation of Plant Germplasm II. Biotech. in Agriculture and Forestry 50: 323–343.
Google Scholar
Sedlák J., Paprštein F. 2012. In vitro establishment and proliferation of red currant cultivars. Hort. Sci. 39: 21–25.
Google Scholar
Wainwright H., Flegmann A.W. 1986. Studies on the micropropagation of Ribes species. Acta Horticulturae 183: 315–322.
Google Scholar
Welander M. 1985. Micropropagation of gooseberry, Ribes grossularia. Sci. Hort. 26(3): 267–272.
Google Scholar
Zabeau M., Vos P., 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application 92402629.7, Publication number 0 534 858 A1.
Google Scholar
Authors
Danuta Kucharskadanuta.kucharska@inhort.pl
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
http://orcid.org/0000-0002-9635-967X
Authors
Danuta WójcikInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
https://orcid.org/0000-0002-3556-8080
Authors
Aleksandra TrzewikInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
https://orcid.org/0000-0003-0378-5440
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