Research on in vitro haploidization of heterozygotic breeding lines of tomato (Solanum lycopersicum L.)
Waldemar Kiszczak
waldemar.kiszczak@gmail.comResearch Institute of Horticulture, Konstytucji 3 Maja 1/3, 96‒100 Skierniewice, Poland (Poland)
https://orcid.org/0000-0002-3925-5233
Urszula Kowalska
Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96‒100 Skierniewice, Poland (Poland)
http://orcid.org/0000-0003-1806-0493
Maria Burian
Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02‒096 Warsaw, Poland (Poland)
http://orcid.org/0000-0002-5891-6869
Abstract
Experiments were made in order to examin the influence of various factors on the induction of androgenesis in heterozygous breeding material of tomato. The factors like: length of buds the manner of sterilization, type and the composition of induction media, genotype and thermal shock were included in conducted experiments. Most of all sterile cultures with the highest number of anther-derived callus were obtain by applying 2,5% calcium hypochlorite for 5 minutes. Anther-derived calli was obtained in 9 breeding lines from 18 used genotypes. The induction medium with the composition based on B5 medium with the addition of 750 mg L-1 calcium chloride and 100 g L-1 sucrose proved to be the best for inducing androgenesis. The addition of thidiazuron and NAA to this medium in the following season and silver nitrate in the other experiminet improved the efficiency of this process, which was depended on the genotype. Despite the lack of statistically significant differences, the highest number of anther-derived calli was obtained when anthers were cooled for 2 days in +4oC in the refrigerating chamber.
Keywords:
genotype, microsporogenesis, phytohormones, sterilization, thermal shock, tomatoReferences
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Authors
Waldemar Kiszczakwaldemar.kiszczak@gmail.com
Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96‒100 Skierniewice, Poland Poland
https://orcid.org/0000-0002-3925-5233
Authors
Urszula KowalskaResearch Institute of Horticulture, Konstytucji 3 Maja 1/3, 96‒100 Skierniewice, Poland Poland
http://orcid.org/0000-0003-1806-0493
Authors
Maria BurianInstitute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02‒096 Warsaw, Poland Poland
http://orcid.org/0000-0002-5891-6869
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Copyright (c) 2021 Waldemar Kiszczak, Urszula Kowalska, Maria Burian
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