Evaluation of cannabinoid content in selected varieties of Cannabis sativa L.

Grażyna Mańkowska

sekretariat@iwnirz.pl
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)

Aleksandra Luwańska


Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)

Karolina Wielgus


Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)

Jan Bocianowski


Uniwersytet Przyrodniczy w Poznaniu (Poland)

Abstract

The study rated the cannabinoid content in hemp varieties of Cannabis sativa L. that have been obtained from Gene Bank of Institute of Natural Fibres and Medicinal Plants in Poznań. The experiment with hemp was conducted in 2014 at the Experimental Farm in Pętkowo (Wielkopolska Region). After collecting the plant material analysis of the content of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) was performed in the six varieties of hemp of European and Asian origins. For these parameters correlation coefficients were calculated and analysis of variance was performed using the software STATISTICA 12. The results showed significant differences in the contents of THC and CBD, and the relationship between the two cannabinoid contents in the hemp varieties.

Supporting Agencies

The presented work received financial support from the National Center for Research and Development (contract no. INNOMED/I/11/NCBR/2014) from the funds of the Innovative Economy Operational Program under the European Regional Development Fund

Keywords:

cannabidiol, varieties of hemp, tetrahydrocannabinol

Białousowa J., Bartosik A., Kurhański M., Nagórski A., Tumalewicz B. 1958. Konopie, rośliny włókniste (Fibrous Plants). PWRil, Warszawa: 193 — 323.
Google Scholar

Bosca I., Karus M. 1997. Der Hanfanbau. Heidelberg, C. F. Muller: 195.
Google Scholar

De Backer. B., Debrus B., Lebrun, P., Theunis L., Dubois N., Decock L., Verstraete A., Hubert P., Charlier C. 2009. Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative of major cannabinoids in cannabis plant material. J. Chromatograf, B: Anal. Tech Biomed. Life Sci. 877: 4115 — 4124.
Google Scholar

Galal A. M., Slade D., Gul W., El-Alfy A. T., Ferreira D., El Sohly M. A. 2009. Naturally occurring and related synthetic cannabinoids and their potential therapeutic applications. Recent Patents on CNS Drug Discovery 4: 112 — 36.
Google Scholar

Grabowska L., Mańkowska G., Baraniecki P. 2004. Zasoby genowe Cannabis sativa L. w Instytucie Włókien Naturalnych w Poznaniu 497: 53 — 57.
Google Scholar

Jądrzak R., Biskupski M. 1999. Zastosowanie wysoko sprawnej chromatografii cieczowej (HPLC) w analizie kannabinoli, Problemy Kryminalistyki 224: 5 — 10.
Google Scholar

Kojoma M., Seki H., Yoshida S., Muranaka T. 2006. DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in „drug-type” and „fibre-type” Cannabis sativa L. Forensic Sci. Int. 159: 132 — 140.
Google Scholar

Lewis R., Ward S., Johnson R., Burns D. T. 2005. Distribution of the principal cannabinoids within bars of compressed cannabis resin. Anal. Chim. Acta 538: 399 — 405.
Google Scholar

Mackie K. 2007. From active ingredients to the discovery of the targets: the cannabinoid receptors. Chern. Biodivers. 4: 1693 — 706.
Google Scholar

Mańkowska G., Strybe M., Chudy M., Luwańska A., Baraniecki P. 2010. Ocena cech użytkowych wybranych odmian konopi Cannabis sativa L. zgromadzonych w Instytucie Włókien Naturalnych i Roślin Zielarskich w 2008 roku. Zeszyty Problemowe Postępów Nauk Rolniczych 555: 529 — 536.
Google Scholar

Mechoulam R., Hanus L. 2013. Inne rodzaje kannabinoidów. [In:] Marihuana i obłęd. Castle D, Murray R M, D’ Souza D C (red.), MediPage. Warszawa: 22 — 28.
Google Scholar

Rymanowski M. 2014. Konopie, przegląd zagadnień związanych z oznaczaniem sumarycznej zawartości delta-9-tetrahydrokannabinolu (D-9-THC) oraz kwasu delta-9-tetrahydrokannabinolowego (D-9-THCA-A) Problemy Kryminalistyki 285 (3): 1 — 22.
Google Scholar

Scheifele G., Dragla P. 2000. 1999. report on environment and latitude effect on THC levels of industrial hemp varieties grown in Ontario. Ontario Ministry of Agriculture, Food and Rural Affairs.
Google Scholar

Scheifele G., Hinz H., Davies K., Calder K-J., Bowman M., Guillemette L. 1999. 1998 Ontario studies in determining the genetic stability, environment and latitude effect on the levels of delta -9 THC for industrial hemp varieties. Ontario Ministry of Agriculture, Food and Rural Affairs.
Google Scholar

Szukalski B. 2005. Kannabinoidy. W: Kompendium wiedzy o środkach uzależniających. Wydawnictwo Instytutu Psychiatrii i Neurologii, Warszawa, 4: 5 — 15 .
Google Scholar

Tkaczyk M., Florek E., Piekoszewski W. 2012. Marihuana i kannabinoidy jako leki. Przegl. Lek. 10: 1095 — 1097.
Google Scholar

Wielgus K., Przewoźna J., Mańkowska G., Grabowska L., Podralska M., Słomski R. 2010. Polimorfizm genu syntazy THCA w zasobach genowych konopi siewnych — Cannabis sativa L. zgromadzonych w Instytucie Włókien Naturalnych i Roślin Zielarskich. Zeszyty Problemowe Postępów Nauk Rolniczych, 555: 457 — 464.
Google Scholar


Published
2015-09-30

Cited by

Mańkowska, G. (2015) “Evaluation of cannabinoid content in selected varieties of Cannabis sativa L”., Bulletin of Plant Breeding and Acclimatization Institute, (277), pp. 79–86. doi: 10.37317/biul-2015-0012.

Authors

Grażyna Mańkowska 
sekretariat@iwnirz.pl
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland

Authors

Aleksandra Luwańska 

Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland

Authors

Karolina Wielgus 

Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland

Authors

Jan Bocianowski 

Uniwersytet Przyrodniczy w Poznaniu Poland

Statistics

Abstract views: 165
PDF downloads: 191


License

Copyright (c) 2015 Grażyna Mańkowska, Aleksandra Luwańska, Karolina Wielgus, Jan Bocianowski

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:

  1. Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
  2. Placing on the market, lending or renting the original or copies of the article.
  3. Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
  4. Including the article in a collective work.
  5. Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
  6. Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
  7. Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.

Authors by sending a request for publication:

  1. They consent to the publication of the article in the journal,
  2. They agree to give the publication a DOI (Digital Object Identifier),
  3. They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
  4. They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
  5. They agree to send article metadata to commercial and non-commercial journal indexing databases.

Most read articles by the same author(s)

<< < 1 2 3 4 > >>