Doubled haploid production of winter and spring triticale hybrids using colchicine in anther cultures
Aurelia Ślusarkiewicz-Jarzina
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Aleksandra Ponitka
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Abstract
The aim of this work was to increase the efficiency of doubled haploids production in anther cultures of 6 winter and 6 spring triticale hybrids. The colchicine concentration and the time of its use (0.1 mg/l or 1.0 mg/l colchicine, for 24, 48 and 72 h, respectively) in androgenesis inducing C17 liquid medium were modified. It was found that the optimal parameters of colchicine treatment were 1.0 mg/l for 24 hours, and 0.1 mg/l for 48 hours. Based on flow cytometry analysis, in those conditions an increase in a number of doubled haploids was observed. The increase was observed in 6 forms of winter (average 66.2%) and 2 forms of spring triticale (average of 52.7%), as compared to control conditions (the C17 medium without colchicine), producing of about 30.0% of doubled haploids
Supporting Agencies
Keywords:
colchicine, anther culture, doubled haploids, winter triticale, spring triticaleReferences
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Authors
Aurelia Ślusarkiewicz-Jarzinaoffice@igr.poznan.pl
Instytut Genetyki Roślin PAN w Poznaniu Poland
Authors
Aleksandra PonitkaInstytut Genetyki Roślin PAN w Poznaniu Poland
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