Application of thiophilic chromatography for purification of polyclonal antibodies from rabbit serum
Agnieszka Barnyk
a.barnyk@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin (Poland)
Jerzy Lewosz
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin (Poland)
Krzysztof Treder
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin (Poland)
Włodzimierz Przewodowski
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin (Poland)
Tomasz Pilecki
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin (Poland)
Abstract
A procedure used for purification of antibodies from serum has a significant effect upon their quality and therapy their suitability for diagnostic tests. The commonly used approaches include: (i) ion-exchange chromatography (both on anion and cation exchangers, depending on conditions applied) and (ii) affinity chromatography (on resins charged with the protein A or with the specific antigen). These methods differ in efficacy; amount of time needed and costs for antibodies purification. The study presented herein estimates the suitability of thiophilic chromatography as a cost and time-saving alternative approach for purification of antibodies from rabbit serum.
Keywords:
antibody purification, blood serum, rabbits, thiophilic chromatographyReferences
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Authors
Agnieszka Barnyka.barnyk@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin Poland
Authors
Jerzy LewoszInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin Poland
Authors
Krzysztof TrederInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin Poland
Authors
Włodzimierz PrzewodowskiInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin Poland
Authors
Tomasz PileckiInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie, Zakład Nasiennictwa i Ochrony Ziemniaka, Bonin Poland
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Copyright (c) 2008 Agnieszka Barnyk, Jerzy Lewosz, Krzysztof Treder, Włodzimierz Przewodowski, Tomasz Pilecki
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