Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN, Poznań (Poland)
Karolina Krystkowiak
Instytut Genetyki Roślin PAN, Poznań (Poland)
Anetta Kuczyńska
Instytut Genetyki Roślin PAN, Poznań (Poland)
Michał Rębarz
Instytut Genetyki Roślin PAN, Poznań (Poland)
Abstract
The aim of the study was estimation of genetic variability in hull-less barley mutants as compared to their initial form at the phenotypic and molecular levels. Material for the performed studies constituted hull-less barley mutants obtained as a result of mutagenic treatment of grain of hull-less spring barley line — 1N/86. Two chemomutagens were the mutagenic agents. The phenotypic as well as yield structure traits of the mutants were analyzed on the ground of performed field trial. Genetic variations of the mutants, in comparison to their initial line, were elaborated with the use of multivariate statistics methods and the RAPD molecular method. Statistical analysis of the obtained results indicated that the mutants were characterized by broad spectrum of variation, as compared to the initial line. On ground of the molecular study, the calculated genetic similarity coefficients allowed to select the mutants with the greatest and smallest genetic similarity to the initial line. Genetic similarity was estimated according to the formula given by Nei and the results were used for hierarchical grouping of the analyzed mutants. A result of the grouping was presented on the dendrogram. With the use of calculated phenotypic and genetic distances their correlation coefficient was estimated. As final results, the mutants were chosen with the lowest similarity to the initial line, on the both analyzed levels (phenotypic and molecular).
Keywords:
hull-less barley, genetic variation, genetic similarity, mutants, RAPDReferences
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Authors
Karolina KrystkowiakInstytut Genetyki Roślin PAN, Poznań Poland
Authors
Anetta KuczyńskaInstytut Genetyki Roślin PAN, Poznań Poland
Authors
Michał RębarzInstytut Genetyki Roślin PAN, Poznań Poland
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