Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels

Wojciech Rybiński

office@igr.poznan.pl
Instytut Genetyki Roślin PAN, Poznań (Poland)

Karolina Krystkowiak


Instytut Genetyki Roślin PAN, Poznań (Poland)

Anetta Kuczyńska


Instytut Genetyki Roślin PAN, Poznań (Poland)

Michał Rębarz


Instytut Genetyki Roślin PAN, Poznań (Poland)

Abstract

The aim of the study was estimation of genetic variability in hull-less barley mutants as compared to their initial form at the phenotypic and molecular levels. Material for the performed studies constituted hull-less barley mutants obtained as a result of mutagenic treatment of grain of hull-less spring barley line — 1N/86. Two chemomutagens were the mutagenic agents. The phenotypic as well as yield structure traits of the mutants were analyzed on the ground of performed field trial. Genetic variations of the mutants, in comparison to their initial line, were elaborated with the use of multivariate statistics methods and the RAPD molecular method. Statistical analysis of the obtained results indicated that the mutants were characterized by broad spectrum of variation, as compared to the initial line. On ground of the molecular study, the calculated genetic similarity coefficients allowed to select the mutants with the greatest and smallest genetic similarity to the initial line. Genetic similarity was estimated according to the formula given by Nei and the results were used for hierarchical grouping of the analyzed mutants. A result of the grouping was presented on the dendrogram. With the use of calculated phenotypic and genetic distances their correlation coefficient was estimated. As final results, the mutants were chosen with the lowest similarity to the initial line, on the both analyzed levels (phenotypic and molecular).


Keywords:

hull-less barley, genetic variation, genetic similarity, mutants, RAPD

Abdel-Fattach, El-Sayed A. A. 2005. Improvement of food hull-less barley in Egypt. International Workshop on Food Barley Improvement, 14–17 January 2002, Hammamet, Tunisia. Proccedings: 7 — 12.
Google Scholar

Akhuary S.B., Sinha A.N., Sinha A. K. 1996. The effectiveness and efficiency of chemical mutagens on biological parameters in hull-less variety of Hordeum vulgare (L.) Karan. Neo Botanica 4 (1): 1 — 5.
Google Scholar

Arumugan S., Reddy V. R. K., Asir R., Viswanathan P., Dhamodaran S. 1997. Induced mutagensis in barley. Advances in Plant Science 10 (1): 103 — 106.
Google Scholar

Barua U. M., Chalmers K. J., Hackett C. A., Thomas W. T. B., Powell W., Waugh R. 1993. Identification of RAPD markers linked to the Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71: 177 — 184.
Google Scholar

Beames R. M., Helm J. H., Eggum B.O., Boisen S., Bach Knudsen K. E., Swift M. L. 1996. A comparison of methods for measuring the nutritive value for pigs of a range of hulled and hulless barley cultivars. Anim. Feed Sci. Technol. 62: 189 — 201.
Google Scholar

Bhatty R. S., Brdhal J. D., Christison G. I. 1975. Chemical composition and digestible energy of barley. Can. J. Anim. Sci., 55: 759 — 764.
Google Scholar

Bhatty R. S. 1986. The potential of hull-less barley — review. Cereal Chem., 63: 97 — 103.
Google Scholar

Bhatty R. S. 1993. Nonmalting uses of barley. In: A. MacGregor and R.S.Bhatty (ed), Barley: Chemistry and Technology. AACC: 355 — 417.
Google Scholar

Bhatty R. S. 1996. Nonwheat grains and products — production of food malt from hull-less barley. Cereal Chemistry 73 (1): 75 — 80.
Google Scholar

Bhatty R.S. 1997. Milling of regular and waxy starch hull-less barleys for the production of bran and flour. Cereal Chemistry 74 (6): 693 — 699.
Google Scholar

Bhatty R. S., Rossnagel B. G. 1998. Comparison of pearled and unpearled Canadian and Japanese barleys. Cereal Chemistry 75 (1): 15 — 21.
Google Scholar

Caliński T., Dyczkowska A., Kaczmarek Z. 1976. Algorytmy Biometrii i Statystyki, Zeszyt 5: 77 — 113.
Google Scholar

Campbell S. G., Mehra R. B., Agraval S.K., Chen Y. Z., Abd El Moneim A.M., Khawaja H .I. T., Yadow C. R., Tay J. U., Araya W. A.. 1994. Current status and future strategy in breeding grasspea (Lathyrus sativus L.). Euphytica 73: 167 — 175.
Google Scholar

Ceranka B., Chudzik H., Czajka S., Kaczmarek Z. 1987. Wielozmienna analiza wariancji dla doświadczeń wieloczynnikowych. Algorytmy Biometrii i Statystyki. Zesz. 6: 51 — 60
Google Scholar

Choo T. W., Ho K. H., Martin R. A. 2001. Genetic analysis of hulless x covered cross of barley using doubled-haploids lines. Crop Sci. 41: 1021 — 1026.
Google Scholar

Czembor H. J. 1989. Stan i perspektywy hodowli w Polsce. Biul. IHAR 171-172: 5 — 14.
Google Scholar

Czembor H. J. 1997. Hodowla jęczmienia w Polsce i Europie. Agrotechnika i Wykorzystanie Jęczmienia, Seminarium Naukowe. Puławy, 23–24 października 1997: 41 — 48.
Google Scholar

Dziamba S., Rachoń L. 1988. Zróżnicowanie elementów struktury plonu nagoziarnistych i oplewionych odmian jęczmienia jarego uprawianych w siewie czystym i mieszanym. Biul. IHAR 167: 79 — 85.
Google Scholar

El-Sayed A. A., El-Enein R. A., El-Gamal A. S. 2004. Two new hull-less barley varieties for rainfed in Egypt. New directions for a diverse plant. 4th International Crop Science Congress, Brisbane, Australia, 26 Sep. – 1 Oct. 2004. Ed. — Fischer T., Proccedings: 28
Google Scholar

Eslick R. F., Blake T., Stallknecht G. 1990. Registration of Wanubet a hulless waxy barley germplasm. Crop Science 30 (6): 1371.
Google Scholar

Górny A. 2004. Zarys Genetyki Zbóż (Zarys Genetyki Jęczmienia), IGR PAN, Poznań. Tom 1: 15 — 142.
Google Scholar

Hang A., Burton C. S., Hoffman D. L., Jones B. L. 2000. Random amplified polymorphic primer-generated embryo DNA polymorphism among 16 North American malting barley cultivars. J. Am. Soc. Brew. Chem. 58 (4): 147 — 151.
Google Scholar

Harlan H. V., Martini M. L., Stevens H. 1940. A study on methods in barley breeding. USDA Washington DC, Technical Bull. 720.
Google Scholar

Helm C. V., De Francisco Gaziola S A., Fornazier R. F., Pompeu G. B., Azevedo R. A. 2004. Hull-less barley varieties: storage proteins and amino acid distribution in relation to nutritional quality. Food Biotechnology 18 (3): 327 — 341.
Google Scholar

Kolasińska K., Boros L. 2003. Wartość siewna oplewionych i nieoplewionych nasion jęczmienia jarego. Hod. Rośl.Nasien. 1/2003: 14 — 15.
Google Scholar

Kroth M. A., Ramella M. S., Tagliari C., De Francisco A., Arisi A. C. M. 2005. Genetic similarity of Brazilian hull-less and malting barley varieties evaluated by RAPD markers. Sci. Agric. 62 (1): 36 — 39.
Google Scholar

Kuczyńska A., Milczarski P., Surma M., Masojć P., Adamski T. 2001. Genetic diversity among cultivars of spring barley revealed by random amplified polymorphic DNA (RAPD). J. Appl. Genet. 42 (1): 43 — 48.
Google Scholar

Kuczyńska A., Bocianowski J., Masojć P., Surma M., Adamski T. 2003. Zastosowanie markerów RAPD do określenia podobieństwa genetycznego odmian jęczmienia ozimego (Hordeum vulgare L.). Biul. IHAR 226/227 (1): 81 — 85.
Google Scholar

Kuczyńska A., Bocianowski J., Surma M., Kaczmarek Z., Adamski T. 2004. Relationship between phenotypic and genetic distances in a set of barley breeding lines. 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June, Proc., 33.
Google Scholar

Lockhart H.N., Hurt D.H. 1986. Nutrition of oats. In: Webster (ed.). Oats: chemistry and technology. St. Paul, AACC: 73 — 130.
Google Scholar

McGuire C.F., Hockett. 1981. Effect of awn length and naked caryopsis of malting quality of Betzes barley. Crop Sci. 21: 18 — 21.
Google Scholar

Nagy J.G. 2003. Economic returns to feed barley yield-increasing and disease resistance research at the Alberta Field Crop Development Centre. Canadian J. of Agricultural Economics 51(30: 281 — 298.
Google Scholar

Nam I.H., Lee E.S. 1990. Effect of waxy hulless genes on endosperm quality, yield and its related traits in barley (Hordeum vulgare L.). Upland and Industrial Crops 32(2): 1 — 12.
Google Scholar

Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction. Proceedings of the Academy of Sciences of the USA 76: 5269 — 5273.
Google Scholar

Noworolnik K., Leszczyńska D. 2004. Reakcja nagoziarnistego jęczmienia jarego na gęstość siewu w porównaniu z jęczmieniem oplewionym. Biul. IHAR 233: 99 — 105.
Google Scholar

Paris R. L. 1999. Development of hulless varieties as an improved feed crop. Barley Newsl., 43: 30.
Google Scholar

Przyborowski J.A., Weeden N.F. 2001. RAPD-based assessment of genetic similarity and distance between Lupinus in section Albus. J. Appl. Genet. 42 (4): 55 — 61.
Google Scholar

Rybiński W. 1981. Short-straw forms of spring barley (Hordeum vulgare L.) obtained as a result of MNUA-induced mutations. Genetica Polonica 22 (3): 271 — 287.
Google Scholar

Rybiński W., Patyna H., Przewoźny T. 1993. Mutagenic effect of laser and chemical mutagens in barley (Hordeum vulgare L.). Genetica Polonica 34 (4): 337 — 343.
Google Scholar

Scholz F. 1965. Experiments on the use of induced mutants to hybridization breeding in barley. Induction of Mutations and the Mutation Process, Praha: 73 — 79.
Google Scholar

Shewry P.R. 1993. Barley seeds proteins. In: MacGregor and Bhatty, Barley: Chemistry and technology. St. Paul, AACC: 1 — 5.
Google Scholar

Takahaski R.H., Inamura H., Matsumoto T. 1961. Effect of genes for covered and naked kernels on some agronomic characters in barley. I. Comparison of two covered and their naked mutants. Ber. Ohara Inst. Landwirtsch. Biol., Okayama Univ. 11: 385 — 392.
Google Scholar

Thair M., Shevtsov V. 1994. New hull-less barley germplasm for high — altitude areas. Rachis 13(1/2): 43 — 46.
Google Scholar

Trop J., Doll H., Haahr V. 1981. Genotypic and environmental influence upon the nutritional composition of barley grain. Euphytica 30 (30; 719 — 728.
Google Scholar

Welsh J., McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213 — 7218.
Google Scholar

Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531 — 6535.
Google Scholar

Vaidya S. M., Mahabalran M. 1989. Karan 3 and Karan 16 — two high yielding hull-less barley varieties. Indian Farming 39 (7): 37 — 38.
Google Scholar

Xue Q., Wang L., Newman R. K., Newman C. W., Graham H. 1997. Influence of hulless, waxy starch and short-awn genes on the composition of barleys. J. of Cereal Sci. 26(2): 251 — 257.
Google Scholar

Yang. W. Z., Beauchemin B. I., Farr B. I., Rode L. M. 1997. Comparison of barley, hull-less barley, and corn in the concentrate of dairy cow. J. Dairy Sci. 80: 2885 — 2895.
Google Scholar


Published
2007-09-30

Cited by

Rybiński, W. (2007) “Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels”, Bulletin of Plant Breeding and Acclimatization Institute, (245), pp. 113–127. doi: 10.37317/biul-2007-0028.

Authors

Wojciech Rybiński 
office@igr.poznan.pl
Instytut Genetyki Roślin PAN, Poznań Poland

Authors

Karolina Krystkowiak 

Instytut Genetyki Roślin PAN, Poznań Poland

Authors

Anetta Kuczyńska 

Instytut Genetyki Roślin PAN, Poznań Poland

Authors

Michał Rębarz 

Instytut Genetyki Roślin PAN, Poznań Poland

Statistics

Abstract views: 144
PDF downloads: 34


License

Copyright (c) 2007 Wojciech Rybiński, Karolina Krystkowiak, Anetta Kuczyńska, Michał Rębarz

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:

  1. Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
  2. Placing on the market, lending or renting the original or copies of the article.
  3. Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
  4. Including the article in a collective work.
  5. Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
  6. Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
  7. Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.

Authors by sending a request for publication:

  1. They consent to the publication of the article in the journal,
  2. They agree to give the publication a DOI (Digital Object Identifier),
  3. They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
  4. They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
  5. They agree to send article metadata to commercial and non-commercial journal indexing databases.

Most read articles by the same author(s)

<< < 1 2 3 > >>