Acccleration of the determination of potential germinability of winter wheat seed by a more rapid tetrazolium method
Janina Budzyńska
Instytut Hodowli i Aklimatyzacji Roślin - Radzików, Zakład Metodyki Kontroli Materiału Siewnego (Poland)
Krzysztof Niemyski
Instytut Hodowli i Aklimatyzacji Roślin - Radzików, Zakład Metodyki Kontroli Materiału Siewnego (Poland)
Abstract
Investigations were carried out on a method of shortening the time of viability assessment of cereal seed by the tetrazolium topographical (TT) method. The influence of temperature, acidity of the tetrazolium solution and incubation time of excised embryos on the intensity of staining were tested. The range of variables covered the temperatures: 25°, 30°, 35°. and 40°C, the acidity of pH 6.0 to 8.0 and times of incubation of 1, 2, 3, 4 and 5 hours. A 1% 2, 3, 5-triphenyltetrazolium chloride solution (TTC) was used in all cases.
Taking into account the obtained results a new more rapid version of the TT method is proposed. The excised embryos should be incubated in a 1% TTC buffered solution having and acidity of pH 7.2 - 7.4 at the temperature 40°C for 3 hours.
Determinations of germinability and viability by the standard TT procedure, the new quicker method were carried out on 280 samples of winter wheat seed, representing lots from the 1975 and 1976 harvests. The analysis of variance proved that the new method and the standard TT method arc equal on the 0,001 level. As the two methods can be considered as equivalent, the most advantageous is the proposed one, allowing for the final assessment of seed viability one day earlier.
Keywords:
seed science, winter wheat, Triticum aestivumReferences
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Authors
Janina BudzyńskaInstytut Hodowli i Aklimatyzacji Roślin - Radzików, Zakład Metodyki Kontroli Materiału Siewnego Poland
Authors
Krzysztof NiemyskiInstytut Hodowli i Aklimatyzacji Roślin - Radzików, Zakład Metodyki Kontroli Materiału Siewnego Poland
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Copyright (c) 1980 Janina Budzyńska, Krzysztof Niemyski
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