Comparison of aniline blue staining and immunoblotting methods for detecting endophytic fungi in grass seeds
Barbara Wiewióra
b.wiewiora@ihar.edu.plZakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Maria Prończuk
Samodzielna Pracownia Traw i Roślin Motylkowych, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The objective of the study was to compare the efficiency of and the input of labour and money in aniline blue staining and immunoblotting assays used to identify endophytic fungi infesting grass seed. Seeds of 17 cultivars, strains and ecotypes of meadow fescue (Festuca pratensis L.), red fescue (F. rubra L.), perennial ryegrass (Lolium perenne L.) and tufted hairgrass (Deschampsia caespitosa (L.) P. Beauv.) were tested. Detectability of endophytes using the two methods was comparable. However, the aniline blue staining was found to be a very laborious and time-consuming method. Moreover, great experience of the researcher is needed to recognize the endophyte mycelium under the microscope. Furthermore, the method requires additional laboratory equipment to protect the staff against the harmful effects of some reagents. The immunoblotting method is comparatively more expensive, but it is evidently easier and less labour- and time-consuming.
Keywords:
aniline blue, endophytic fungi, grass seed, immunoblot method, staining methodReferences
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Authors
Barbara Wiewiórab.wiewiora@ihar.edu.pl
Zakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Maria PrończukSamodzielna Pracownia Traw i Roślin Motylkowych, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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