Using SSR markers for genetic diversity testing in barley genotypes with different sensitivity against Fusarium Head Blight
Ivana Ježíšková
int.office@mendelu.czMendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Jan Bednář
Mendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Hana Nevimová
Mendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Vlasta Wegscheiderová
Mendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Abstract
Fusarium Head Blight (FHB) or scab is a devastating disease of cereals widely spread all over the world and is caused by members of the genus Fusarium (F. culmorum, F. graminearum, and other ones). The FHB infection of barley may result in lower yields and in the accumulation of dangerous secondary metabolites of fungi — mycotoxins in the kernels (e.g. deoxynivalenol, DON). The accumulated mycotoxins have a negative effect on the safety and technological quality of the grain. The objective of the study was to test 7 parental genotypes of spring barley (with a recognised resistance or susceptibility to FHB) with SSR markers (Simple Sequence Repeats). We tested the following parental genotypes: Chevron, Zao Zhou 3, PEC210, CI4196, 6NDRFG-1 (resistant genotypes), and PI383933 and Foster (susceptible genotypes). The results of the analyses will be used to test 22 DH lines, which were derived after crossing the tested parental genotypes (resistant × susceptible). By now, a total of 35 SSR markers of parental genotypes were tested out of the total number of 60 selected microsatellite markers; 29 of the tested SSR markers showed a polymorphous character in the parental genotypes. The remaining 6 ones were monomorphous and will not be used to test the DH lines. Partial results of the analyses were elaborated into the form of a binary matrix and transformed into the form of a dendrogram, which characterised genetic affinity of the tested genotypes.
Supporting Agencies
Keywords:
FHB, barley, microsatellitesReferences
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Authors
Ivana Ježíškováint.office@mendelu.cz
Mendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
Authors
Jan BednářMendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
Authors
Hana NevimováMendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
Authors
Vlasta WegscheiderováMendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
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Copyright (c) 2006 Ivana Ježíšková, Jan Bednář, Hana Nevimová, Vlasta Wegscheiderová
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