Genetic variability of storage proteins in some winter triticale (X Triticosecale Wittmack) varieties
Miłosz Smolik
dziekanat.wksir@zut.edu.plZakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie (Poland)
Danuta Rzepka-Plevneš
Zakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie (Poland)
Katarzyna Grys
Zakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie (Poland)
Abstract
The aim of this work was to determine the genetic polymorphism of 15 varieties of triticale (Dagro, Moniko, Salvo, Fidelio, Lamberto, Pronto, Kitaro, Woltario, Tevo, Disco, Moreno, Presto, Prego, Chrono, Lasko) on the basis of SDS-PAGE technique. From 19 (Presto) to 27 (Salvo, Fidelio and Lambert) protein bands were observed on the electrophoregrams obtained for the abave triticale varieties. Their weight varied from 105.8 to 18.4 kDa. According to the weight they were classified in four protein fractions: HMW, ω, γ75 and γ40 with weight: > 100, 100 — 75, 75 — 40, and < 40 kDa, respectively. In the fraction including the heaviest proteins (HMW) the majority of varieties showed the presence of 2 polypeptides of 105.8 and 101 kDa. The exception were varieties Dagro, Lamberto and Kitaro because they showed the presence of 1 polypeptide of 101 kDa. In fraction ω the presence of 1 (Kitaro) to 6 (Lamberto) protein bands was observed. The bands of 99.2, 95.09, 87.9, 83.44 and 81.6 kDa appeared to be polymorphic. In this fraction, they were observed on the electrophoregrams in different arrangements. Protein fraction γ75 consisted of 7 to 9 bands of weight ranging from 74.9 to 41.9 kDa. The polypeptides 72.31, 70; 68.8; and 65.11 kDa turned out to be polymorphic. The lightest fraction of the storage proteins (γ40) contained 9 to 12 protein bands of weight ranging from 39.6 to 18.4 kDa. The analysis of the tree of the phylogenetic similarity showed that the examined varieties of triticale were similar to each other in the range from 73.7 to 93.6%. They were divided into 5 different groups of genetic similarities.
Keywords:
SDS-PAGE, gliadins, polymorphism, triticale, varietiesReferences
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Authors
Miłosz Smolikdziekanat.wksir@zut.edu.pl
Zakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie Poland
Authors
Danuta Rzepka-PlevnešZakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie Poland
Authors
Katarzyna GrysZakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie Poland
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Copyright (c) 2005 Miłosz Smolik, Danuta Rzepka-Plevneš, Katarzyna Grys
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