Electrophoretic variability of storage protein and morphological features of some rye species (Secale sp.)
Agata Markowska
ktchniis@zut.edu.plInstytut Technologii Chemicznej Nieorganicznej i Inżynierii Środowiska, Politechnika Szczecińska, Szczecin (Poland)
Danuta Rzepka-Plevneš
Zakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie (Poland)
Abstract
The studies included 8 wild species of rye (Secale sp.): Secale afghanicum (Vav.), Secale ancestrale (Zhuk.), Secale dighoricum (Vav.), Secale kuprijanovii (Grossh.), Secale montanum (Guss.), Secale segatale (Roshev.), Secale silvestre (Host.), Secale vavilovii (Grossh.). Phenotypic differences were determined on the basis of morphological features of rye plants and grain, and genetic differences were measured based on polymorphism of storage proteins, assayed with the SDS-PAGE electrophoresis. To differentiate the rye species, morphological features of grain, such as: length, width and area of kernels, were found to be better measures than morphological features of plants. The results of experiments showed that among the tested genotypes there were differences in the number and weight of polypeptides and polypeptide patterns in particular fractions. The similarity between the genotypes ranged from about 29% to 55%. S. ancestrale and S. segetale were found to be the most similar genetically.
Keywords:
storage proteins, electrophoresis, variability, ryeReferences
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Authors
Agata Markowskaktchniis@zut.edu.pl
Instytut Technologii Chemicznej Nieorganicznej i Inżynierii Środowiska, Politechnika Szczecińska, Szczecin Poland
Authors
Danuta Rzepka-PlevnešZakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie Poland
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