The use of in vitro gynogenesis for obtaining sugar beet dihaploids
Maria Gośka
m.goska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy (Poland)
Teresa Krysińska
Kutnowska Hodowla Buraka Cukrowego, Stacja Hodowli Roślin, Straszków (Poland)
Krystyna Strycharczuk
Kutnowska Hodowla Buraka Cukrowego, Stacja Hodowli Roślin, Straszków (Poland)
Abstract
The in vitro cultures of unpollinated ovules can be successfully used for obtaining dihaploids from tetraploid pollinators. Dihaploid lines from tetraploid pollinators give the feasibility to obtain a new variation from high heterozygous tetraploid materials. Ovules of tetraploid pollinators of sugar beet — N-type, ZN-type and Z-type were used as plant material in the studies. They were incubated on initial medium (MS) with 1 mg·l-1 BAP and 0.1 mg·l-1 NAA. A parental plant genotype affected the ability of gynogenesis. The percentage of plants obtained from ovules varied between 0.2 and 2%. The higher ability of plant regeneration was observed with ZN-type, the lower one was observed with Z-type and N-type. Analysis of ploidy levels of plants obtained from unpollinated ovules showed a 98.5% share of diploids and a 1.5% share of tetraploids. Dihaploid lines obtained from ovules of various genotypes differed in morphological traits.
Keywords:
dihaploids, in vitro culture, sugar beet, unpollinated ovulesReferences
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Authors
Maria Gośkam.goska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy Poland
Authors
Teresa KrysińskaKutnowska Hodowla Buraka Cukrowego, Stacja Hodowli Roślin, Straszków Poland
Authors
Krystyna StrycharczukKutnowska Hodowla Buraka Cukrowego, Stacja Hodowli Roślin, Straszków Poland
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Copyright (c) 2004 Maria Gośka, Teresa Krysińska, Krystyna Strycharczuk
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