Resistance tests of interspecific hybrids of the tribe Brassiceae to infection caused by the Leptosphaeria sp. and Alternaria sp.

Elżbieta Starzycka-Korbas

e.starzycka-korbas@ihar.edu.pl
IHAR — PIB Oddział w Poznaniu, Pracownia Metod Hodowli Odpornościowej (Poland)

Michał Starzycki


IHAR — PIB Oddział w Poznaniu, Pracownia Metod Hodowli Odpornościowej (Poland)

Piotr Kamiński


Instytut Ogrodnictwa, Zakład Hodowli Roślin Ogrodniczych, Pracownia Genetyki i Hodowli Roślin Warzywnych w Skierniewicach (Poland)

Mirosława Dabert


Uniwersytet Adama Mickiewicza w Poznaniu (Poland)

Wojciech Rybiński


Instytut Genetyki Roślin PAN w Poznaniu (Poland)

Grzegorz Budzianowski


HR Strzelce Sp. z o.o. Grupa IHAR (Poland)

Michał Stefanowicz


HR Strzelce Sp. z o.o. Grupa IHAR (Poland)

Abstract

The paper presents the results of studies on resistance of interspecies hybrids from the Brassiceae caused by Leptosphaeria sp. and Alternaria sp. pathogens. 159 intercross hybrid genomes were obtained, of which 52 genotypes were cloned in vitro and in vivo. The genotypes were searched from the Brassiceae less resistant to infestation by Leptosphaeria sp. and Alternaria sp. 49 oilseed rape lines were tested, of which a small proportion showed increased resistance to both pathogens. DNA analyses of ten pathogens used for inoculation were also performed. The resistance of seedlings (infection index for the 12 objects) obtained from interspecific hybrids and also control and donor plants of oilseed rape were tested for infection caused by pathogens of the genus Leptosphaeria and Alternaria. Selected genotypes with low infection indices were transferred to resistance breeding.


Keywords:

DNA of pathogen, interspecific cross Brassiceae, resistance to Leptosphaeria sp. and Alternaria sp.

Balesdent M. H., Barbetti M., Li Hua, Sivasithamparam K., Gout L., Rouxel T. 2005. Analysis of Leptosphaeria maculans race structure in a worldwide collection of isolates. Phytopathology 95: 1061 — 1071.
Google Scholar

Boba A. Kulma, Kostyn K., Starzycki M., Starzycka E., Szopa J. 2011. The influence of carotenoid biosynthesis modification on the Fusarium culmorum and Fusarium oxysporum resistance in flax. Physiological and Molecular Plant Pathology 76: 39 — 47.
Google Scholar

Cai X., Yang L., Zhang J., Li G. Q. 2014. First Report of Leptosphaeria biglobosa Causing Black Leg on Raphanus sativus in Central China. Plant Disease 98(7): 993 — 993.
Google Scholar

Chen Q., Jiang J. R., Zhang G. Z., Cai L., Crous P. W. 2015. Resolving the Phoma enigma. 2015. Studies in Mycology 82: 137 — 217.
Google Scholar

CETIOM, 1990. Techniques for the artificial contamination of oilseed rape in trial plots. Les Points Methodes du CETIOM: 20 — 25.
Google Scholar

Degenhardt K. J., Petrie G. A., Morrall R. A. A. 1982. Effects of temperature on spore germination and infection of rapeseed by Alternaria brassicae, A. brassicicola and A. raphani. Canadian Journal of Plant Pathology 4: 115 — 118.
Google Scholar

Delourme R., Chevre A. M., Brun H., Rouxel T., Balesdent M. H., Dias J., Salisbury P., Renard M., Rimmer S. R. 2006. Major gene and polygenic resistance to Leptosphaeria maculans in oilseed rape (Brassica napus). European Journal of Plant Pathology 114: 41 — 52.
Google Scholar

Douglas W. Heath, Elizabeth D. Earle, Michael H. Dickson. 1994. Introgressing Coldtolerant Ogura Cytoplasm from Rapeseed Choi and Chinese Cabbage. HortScience 29 (3): 202 — 203.
Google Scholar

Fudal I., Ross S., Gout L., Blaise F., Kuhn M. L., Eckert M .R., Cattolico L., Bernard-Samain S., Balesdent M. H., Rouxel T. 2007. Heterochromatin-like regions as ecological niches for avirulence genes in the Leptosphaeria maculans genome: map-based cloning of AvrLm6. Molecular Plant — Microbe Interactions 20: 459 — 470.
Google Scholar

Kamiński P., Podwyszyńska M., Starzycki M., Starzycka-Korbas E. 2016. Interspecific hybridisation of cytoplasmic male-sterile rapeseed with Ogura cytoplasm and Brassica rapa var. pekinensis as a method to obtain male-sterile Chinese cabbage inbred lines. Euphytica, Volume 208: 519 — 534.
Google Scholar

Naumann T. A., Wicklow D. T. 2013. Chitinase modifying proteins from phylogenetically distinct lineages of Brassica pathogens. Physiological and Molecular Plant Pathology 82: 1 — 9.
Google Scholar

Niemann J., Olender M, Wojciechowski A, Tomkowiak A. 2015. Interspecific hybridization between Brassica napus and Brassica rapa ssp. chinensis genotypes through embryo rescue and their evaluation for crossability. BioTechnologia vol. 96 (2): 184 — 191.
Google Scholar

Plissonneau C., Daverdin G., Ollivier B., Blaise F., Degrave A., Fudal I., Rouxel T., Balesdent M. H. 2016. A game of hide and seek between avirulence genes AvrLm4-7 and AvrLm3 in Leptosphaeria maculans. New Phytologist 209: 1613 — 1624.
Google Scholar

Nowicki M., Nowakowska M., Niezgoda A., Kozik E. U. 2012. Alternaria black spot of crucifers symptoms, importance of disease, and perspectives of resistance breeding. Vegetable Crops Research, Bulletin Research Institute of Horticulture 76: 5 — 19.
Google Scholar

Rouxel T., Grandaubert J., Hane J.K., Hoede C., van de Wouw A.P., Couloux A., Dominguez V., Anthouard V., Bally P., Bourras S., Cozijnsen A.J., Ciuffetti L.M., Degrave A., Dilmaghani A., Duret L., Fudal I., Goodwin S.B., Gout L., Glaser N., Linglin J., Kema G.H., Lapalu N., Lawrence C.B., May K., Meyer M., Ollivier B., Poulain J., Schoch C.L., Simon A., Spatafora J.W., Stachowiak A., Turgeon B.G., Tyler B.M., Vincent D., Weissenbach J., Amselem J., Quesneville H., Oliver R.P., Wincker P., Balesdent M.H., Howlett B.J. 2011. Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations. Nature Communications 2: 202.
Google Scholar

Sprague S. J., Balesdent M.H., Brun H., Hayden H. L., Marcroft S. J., Pinochet X., Rouxel T., Howlett B. J. 2006. Major gene resistance in Brassica napus (oilseed rape) is overcome by changes in virulence of populations of Leptosphaeria maculans in France and Australia. European Journal of Plant Pathology 114: 33 — 40.
Google Scholar

Starzycki M., Starzycka E, Pszczoła J. 2007. Development of Alloplasmic Rape. Advances in Botanical Research, Elsevier Ltd. Vol. 45: 313 — 335.
Google Scholar

Starzycka E., Starzycki M. 2008. Najważniejsze wyniki badań nad zgnilizną twardzikową w rzepaku przedstawione na XII Międzynarodowym Kongresie Rzepakowym w Wuhan. Rośliny Oleiste. Oilseed Crops t. XXIX: 281 — 290.
Google Scholar

Starzycka E., Kauzik M., Starzycki M., Cichy H., Budzianowski G., Woś H. 2009. Odporność rzepaku na porażenie przez Leptosphaeria sp. i Sclerotinia sclerotiorum (Lib.) de Bary oceniana w doświadczeniach PDO w dwóch miejscowościach: Małyszynie i Borowie, w latach 2008 i 2009. Rośliny Oleiste. Oilseed Crops t. XXX: 207 — 222.
Google Scholar

Starzycka E., Starzycki M. 2011. In vivo and in vitro investigations on changes taking place under the influence of Sclerotinia sclerotiorum (Lib.) de Bary fungus mycotoxin. Phytopathologia 61: 43 — 49.
Google Scholar

Van de Wouw A. P., Lowe R. G. T., Elliott C. E., Dubois D. J., Howlett B. J. 2014. An avirulence gene, AvrLmJ1, from the blackleg fungus, Leptosphaeria maculans, confers avirulence to Brassica juncea cultivars. Molecular Plant Pathology 15: 523 — 530.
Google Scholar

Wei J., Lin Z., Zhang M., Qin L., Bao Y., Wang J., Rao G. P. 2015. First Report of Alternaria sp. causing Brown Leaf Streak on Sugarcane in China. Plant Disease 99 (8): 1176.
Google Scholar

Winter H. 1999. Blackleg resistance of different origin transferred into Brassica napus. www.regional.org.au/au/gcirc/4/593.htm.
Google Scholar

Yuko Kaneko and Sang Woo Bang. 2014. Interspecific and intergeneric hybridization and chromosomal engineering of Brassicaceae Crops. Breeding Science 64 (1): 14 — 22.
Google Scholar


Published
2018-08-29

Cited by

Starzycka-Korbas, E. (2018) “Resistance tests of interspecific hybrids of the tribe Brassiceae to infection caused by the Leptosphaeria sp. and Alternaria sp”., Bulletin of Plant Breeding and Acclimatization Institute, (282), pp. 123–138. doi: 10.37317/biul-2017-0019.

Authors

Elżbieta Starzycka-Korbas 
e.starzycka-korbas@ihar.edu.pl
IHAR — PIB Oddział w Poznaniu, Pracownia Metod Hodowli Odpornościowej Poland

Authors

Michał Starzycki 

IHAR — PIB Oddział w Poznaniu, Pracownia Metod Hodowli Odpornościowej Poland

Authors

Piotr Kamiński 

Instytut Ogrodnictwa, Zakład Hodowli Roślin Ogrodniczych, Pracownia Genetyki i Hodowli Roślin Warzywnych w Skierniewicach Poland

Authors

Mirosława Dabert 

Uniwersytet Adama Mickiewicza w Poznaniu Poland

Authors

Wojciech Rybiński 

Instytut Genetyki Roślin PAN w Poznaniu Poland

Authors

Grzegorz Budzianowski 

HR Strzelce Sp. z o.o. Grupa IHAR Poland

Authors

Michał Stefanowicz 

HR Strzelce Sp. z o.o. Grupa IHAR Poland

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