Evaluation of onion (Allium cepa L.) accessions for drought tolerance at germination and seedling growth
Urszula Kłosińska
urszula.klosinska@inhort.plPracownia Genetyki i Hodowli Roślin Warzywnych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice (Poland)
https://orcid.org/0000-0003-2690-5986
Abstract
The aim of this study was to assess the effect of water deficiency on germination ability and growth of onion seedlings, and identify promising accessions for use in future breeding programs of new onion varieties with drought tolerance. We evaluated 150 onion lines/cultivars derived from Polish and foreign breeding and seed companies and from the Gene Bank of the Institute of Horticulture in Skierniewice. Experiments were performed under laboratory conditions inducing drought stress by using 18% polyethylene glycol (PEG8000) in the seed germination phase and 10% PEG in the seedling phase. Significant differences in reaction to drought stress were observed among onion accessions, confirmed by the high values of the coefficient of variation (CV) for the assessed parameters: maximum percentage of seedling (56,3%). The received results showed, that water deficiency reduced seed germination the least by 23%, then the root length by 36%, and the fresh weight of seedling and cotyledon length by 52% and 53,5%, respectively. A high correlation (r = 0,81‒0,86) between the features describing the growth of seedlings under drought conditions was found, indicating that water deficit reduced growth of whole seedlings. However, no correlation (r = 0,19‒0,24) between them and germination under drought conditions was observed. Among 150 onion accessions, genotype 171026 showed a high level of drought tolerance, both in seed germination stage and in seedling growth stage.
Supporting Agencies
Keywords:
Allium cepa, polyethylene glycol (PEG), tolerance, water deficitReferences
Addai, I. K., Takyi, H., Oduro, G. (2014). Influence of Bulb Weight at Planting and Drought Stress on Growth and Development of onion (Allium cepa L.) in the Northern Region of Ghana. British Journal of Applied Science & Technology, 4 (14): 2125‒2135.
Google Scholar
Ahmad, S., Ahmad, R., Ashraf, M. Y., Ashraf, M., Waraich, E. A. (2009). Sunflower (Helianthus annuus L.) response to drought stress at germination and growth stages. Pak. J. Bot. 41: 647‒654.
Google Scholar
Albuquerque, F. M. C. D., Carvalho, N. M. D. (2003). Effect of type of environmental stress on the emergence of sunflower (Helianthus annuus L.), soyabean (Glycine max L.) Merril) and maize (Zea mays L.) seeds with different levels of vigor. Seed Sci Technol. 31: 65‒467.
Google Scholar
Bibi, A., Sadaqat, H. A., Tahir, M. H. N., Akram, H. M. (2012). Screening of sorghum (Sorghum bicolor Var Moench) for drought tolerance at seedling stage in polyethylene glycol. J. Anim. Plant Sci, 22 (3): 671‒678.
Google Scholar
Brdar-Jokanović, M., Zdravković, J. (2015). Germination of tomatoes under PEG-induced drought stress. Ratar. i Povrtar, 52 (3): 108‒113.
Google Scholar
Hanci, F., Cebeci, E. (2015). Comparison of salinity and drought stress effects on some morphological and physiological parameters in onion (Allium cepa L.) during early growth phase. Bulgarian Journal of Agricultural Science, 21 (6): 1204‒1210.
Google Scholar
Hohl, M., Schopfer, P. (1991). Water relations of growing maize coleoptiles. Comparison between mannitol and polyethylene glycol 6000 as external osmotica for adjusting turgor pressure. Plant Physiol. 95: 716‒722.
Google Scholar
Kavas, M., Baloglu, M. C., Akca, O., Kose, F. S., Gorcay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology 37: 491‒498.
Google Scholar
Kłosińska, U., Kozik, E. U., Treder, W., Klamkowski, K. (2016). Differential effects of drought stress on germination and seedling growth of cucumber accessions. In: Kozik, E. U., Paris, H. S. (eds) Proceedings of Cucurbitaceae 2016. Warsaw: 217‒221.
Google Scholar
Kłosińska, U., Nowakowska, M., Szczechura, W., Nowak, K., Treder, W., Klamkowski, K., Wójcik, K. (2019). Analiza genetycznych i biochemicznych podstaw tolerancji cebuli (Allium cepa L.) na stres niedoboru wody. Biuletyn Inst. Hodowli i Aklimatyzacji Roślin NR 286: 309‒312.
Google Scholar
Kolasińska, K. (2009). Wartość siewna i reakcja ziarniaków zbóż jarych wyprodukowanych na ekologicznych plantacjach nasiennych na stres suszy symulowanej glikolem polietylenowym. Biul. Instyt. Hod. i Aklimat. Rośl. Nr 251: 53‒66.
Google Scholar
Michel, B. E. (1983). Evaluation of the water potentials of solutio of polyethylene glycol 8000 both in the absence and presence of other solutes. Plant Physiol. 72: 66‒70.
Google Scholar
Muscolo, A., Sidari, M., Anastasi, U., Santonoceto, C., Maggio, A. (2014). Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions, 9 (1): 354‒363.
Google Scholar
Prokic, L. J., Stikic, R. (2012). Effects of different drought treatments on root and shoot development of the tomato wildtype and flacca mutant. Arch. Biol. Sci. 63 (4): 1167‒1171.
Google Scholar
Tuna, A. L., Kaya, C., Ashraf, M. (2010). Potassium sulfate improves water deficit tolerance in melon plants grown under glasshouse conditions. J Plant Nutr 33: 1276–1286.
Google Scholar
Türkan, İ., Bor, M., Özdemir, F., Koca, H. (2004). Differential responses of lipid peroxidation and antioxidants in the leaves of drought tolerant P. acutifolius gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168: 223–231.
Google Scholar
Wang, H., Ma, J., Li X., Zhang, R., Li, Y. (2013). Study on drought resistance and screening of the drought resistance assessment indexes at germinating stage of rice. Southwest China Journal of Agricultural Sciences, 17 (5): 594‒599.
Google Scholar
Ziernicka-Wojtaszek, A. (2015). Klimatyczny bilans wodny na obszarze Polski w świetle współczesnych zmian klimatu. Woda-Środowisko-Obszary Wiejskie, T. 15. Z. 4 (52): 93‒100.
Google Scholar
Zhang, N., Zhao, B., Zhang, H. J., Weeda, S., Yang, C., Yang, Z. C., et al. (2013). Melatonin promotes water‐stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L.). Journal of Pineal Research, 54 (1): 15‒23.
Google Scholar
Zhu, J., Kang, H., Tan, H., Xu, M. (2006). Effects of drought stresses induced by polyethylene glycol on germination of Pinus sylvestris var. mongolica seeds from natural and plantation forests on sandy land. Journal of Forest Research, 11 (5): 319‒328.
Google Scholar
Authors
Urszula Kłosińskaurszula.klosinska@inhort.pl
Pracownia Genetyki i Hodowli Roślin Warzywnych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice Poland
https://orcid.org/0000-0003-2690-5986
Statistics
Abstract views: 272PDF downloads: 270 PDF downloads: 19
License
Copyright (c) 2020 Urszula Kłosińska
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Wojciech Szczechura, Marzena Nowakowska, Urszula Kłosińska, Katarzyna Nowak, Krzysztof Rutkowski, Studies of cytological and biochemical mechanisms of plant resistance in the pathosystems tomato — Phytophthora infestans and cucumber — Pseudoperonospora cubensis , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Marzena Nowakowska, Urszula Kłosińska, Katarzyna Nowak, Wojciech Szczechura, Studies on the development of molecular methods for identifying genes responsible for important traits of tomato , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Urszula Kłosińska, Marzena Nowakowska, Wojciech Szczechura, Katarzyna Nowak, Waldemar Treder, Krzysztof Klamkowski, Katarzyna Wójcik , Analysis of genetic and biochemical basis of onion (Allium cepa L.) tolerance to water deficit stress , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Urszula Kłosińska, Marzena Nowakowska, Wojciech Szczechura, Katarzyna Nowak, Waldemar Treder, Krzysztof Klamkowski, Katarzyna Wójcik , Development of genetic, physiological and biochemical basis of cucumber tolerance to water deficit stress , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
