Development of sensitive methods for detection of the most important potato viruses

Krzysztof Treder

k.treder@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii (Poland)

Mateusz Mielczarek


Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii (Poland)

Anna Pawłowska


Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii (Poland)

Bogumiła Zacharzewska


Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii (Poland)

Maria Fedczak


Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii (Poland)

Supporting Agencies

Prace zostały wykonane w ramach badań podstawowych na rzecz postępu biologicznego w produkcji roślinnej na podstawie decyzji Ministra Rolnictwa i Rozwoju Wsi nr HOR.hn.802.19.2018, Zadanie 58.

Keywords:

test ELISA, RT-PCR, RT-LAMP, wirusy ziemniaka, wykrywanie, ziemniak

Boonham N., Glover R., Tomlinson J., Mumford R. 2008. Exploiting generic platform technologies for the detection and identification of plant pathogens. Eur. J. Plant Pathol. 121: 355 — 363.
Google Scholar

Clark M. F., Adams A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34: 475 — 483.
Google Scholar

Du Z., Chen J. and Hiruki C. 2006. Optimization and application of a multiplex RT-PCR system for simultaneous detection of five potato viruses using 18S rRNA as an internal control. Plant Disease 90: 185 — 189.
Google Scholar

Hill S. A., Jackson E. A. 1984. An investigation of the reliability of ELISA as a practical test for detecting potato leafroll virus and potato virus Y in tubers. Plant Pathol. 33: 21 — 26.
Google Scholar

Przewodowska A., Zacharzewska B., Chołuj J., Treder K. 2015. A one-step, real-time reverse transcription loop-mediated isothermal amplification assay to detect Potato virus Y. Am. J. Potato Res., 92: 303 — 311.
Google Scholar

Singh R. P., Kurz J, Boiteau G., Bernard G. 1995. Detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application. J. Virol. Methods 55: 133 — 43.
Google Scholar

Syller J. Detection of potato leaf roll virus in intact sprout disks by enzyme-linked immunosorbent assay. Journal of Phytopathology, 121: 58 — 64.
Google Scholar

Treder K., Chołuj J., Zacharzewska B., Babujee L., Mielczarek M., Burzyński A., Rakotondrafara A. 2018. Optimization of a magnetic capture RT-LAMP assay for fast and real-time detection of potato virus Y and differentiation of N and O serotypes. DOI:10.1007/s00705-017-3635-3, Archives of Virology 163: 447 — 458.
Google Scholar

Treder K., Chołuj J., Zacharzewska B., Mielczarek M. 2017. Detection of potato virus Y (PVY) by reverse-transcription loop-mediated nucleic acid amplification (RT-LAMP). Plant Breed. Seed Sci. 75: 77 — 85.
Google Scholar

Treder K., Przewodowski W., Barnyk A. 2009. Factors influencing detection of potato leafroll virus and potato virus Y in potato tuber extracts. Plant Breed. Seed Sci. 59: 65 — 74.
Google Scholar

Zacharzewska B., Przewodowska A., Treder K. 2014. The adaptation of silica capture RT-PCR for the detection of Potato Virus Y. Am. J. Potato Res., 91: 525 — 531.
Google Scholar


Published
2019-11-30

Cited by

Treder, K. . (2019) “Development of sensitive methods for detection of the most important potato viruses”, Bulletin of Plant Breeding and Acclimatization Institute, (286), pp. 261–264. doi: 10.37317/biul-2019-0059.

Authors

Krzysztof Treder 
k.treder@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii Poland

Authors

Mateusz Mielczarek 

Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii Poland

Authors

Anna Pawłowska 

Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii Poland

Authors

Bogumiła Zacharzewska 

Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii Poland

Authors

Maria Fedczak 

Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, Oddział w Boninie, Pracownia Diagnostyki Molekularnej i Biochemii Poland

Statistics

Abstract views: 174
PDF downloads: 248


License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:

  1. Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
  2. Placing on the market, lending or renting the original or copies of the article.
  3. Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
  4. Including the article in a collective work.
  5. Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
  6. Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
  7. Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.

Authors by sending a request for publication:

  1. They consent to the publication of the article in the journal,
  2. They agree to give the publication a DOI (Digital Object Identifier),
  3. They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
  4. They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
  5. They agree to send article metadata to commercial and non-commercial journal indexing databases.