Gene pyramiding — a tool commonly used in breeding programs breeding programs
Aleksandra Pietrusińska
a.pietrusinska@ihar.edu.plInstytut i Hodowli i Aklimatyzacji Roślin, Radzików, Krajowe Centrum Roślinnych Zasobów Genowych, Pracownia Gromadzenia i Oceny Roślin (Poland)
Jerzy H. Czembor
Instytut i Hodowli i Aklimatyzacji Roślin, Radzików, Krajowe Centrum Roślinnych Zasobów Genowych, Pracownia Gromadzenia i Oceny Roślin (Poland)
Abstract
The main purpose of crop production is to achieve the highest possible yield while minimizing use of pesticides. Growing varieties with beneficial traits, also with a high potential of yield is closely connected with their resistance. At present, many tools of classical and molecular genetics, which can be successfully used to improve plant disease resistance, are available. The aim of this study was to present the gene pyramids, obtained through various research projects conducted by the Laboratory of Applied Genetics (Department of Plant Breeding and Genetics, Plant Breeding and Acclimatization Institute — National Research Institute, Radzików). Since 1st of February 2016 this research will be continued in the Laboratory of Plant Collection and Evaluation (National Centre for Plant Genetic Resources, Plant Breeding and Acclimatization Institute — National Research Institute, Radzików). Profile of the work involves improving winter wheat resistance to powdery mildew and leaf rust.
Supporting Agencies
Keywords:
cultivar resistance genes, powdery mildew, gene pyramids, leaf rust, wheatReferences
Apolinarska B., Gruszecka D. 2001. Transfer genów z Dasypyrum villosum (Haynaldia villosa L.) do Secale cereale L. Biotechnologia 2 (53): 63 — 65.
Google Scholar
Bennett F. G. A. 1984. Resistance to powdery mildew in wheat: A review of its use in agriculture and breeding programmes. Plant. Pathol. 33: 279 — 300.
Google Scholar
Blanco A., Gadaleta A., Cenci A., Carluccio A. V., Abdelbacki A. M. M., Simeone, R. 2008. Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor. Appl. Genet. 117: 135 — 142.
Google Scholar
Brown-Guedira G. L., Singh S., Fritz, K. 2003. Performance and mapping of leaf rust resistance transferred to wheat from Triticum timopheevii subsp. armeniacum. Phytopathology 93 (7): 784 — 789.
Google Scholar
Cenci A., D’Ovidio R., Tanzarella O. A., Ceoloni C., Porceddu E. 1999. Identification of molecular markers linked to Pm13, and Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theor. Appl. Genet. 98: 448 — 454.
Google Scholar
Charpe A., Koul S., Gupta S. K., Singh A., Pallavil J. K., Prabhu K. V. 2012. Marker assisted gene pyramiding of leaf rust resistance genes Lr24, Lr28 and Lr9 in a bread wheat cultivar HD2329.
Google Scholar
Chełkowski J., Koczyka G. 2005 a. Genomika i bioinformatyka roślin. Rozprawy i Monografie. IGR PAN, Poznań: 139 — 157.
Google Scholar
Chełkowski J., Stępień Ł., Strzembicka A. 2005 b. Ocena podatności pszenicy ozimej na rdzę brunatną oraz poszukiwanie źródeł odporności. Acta Agrobotanica 58 (1): 143 — 152.
Google Scholar
Chełkowski J., Koczyka G. 2005 a. Genomika i bioinformatyka roślin. Rozprawy i Monografie. IGR PAN, Poznań: 139 — 157.
Google Scholar
Chełkowski J., Stępień Ł. 2001. Molecular markers for leaf rust resistance genes in wheat. J. Appl. Genet. 42 (2): 117 — 126.
Google Scholar
Chen P. D., Qi L. L., Zhou B., Zhang S. Z., Liu D. J. 1995. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor. Appl. Genet. 91: 1125 — 1128.
Google Scholar
Chen X. M., Luo, Y. H., Xia X.C., Xia L.Q., Chen, X., Ren Z. L., He Z. H., Jia J. Z. 2005. Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breed. 124: 225 — 228.
Google Scholar
Czajowski G., Strzembicka A., Karska K. 2011. Wirulencja populacji Puccinia triticina sprawcy rdzy brunatnej pszenicy i pszenżyta. Konferencja Nauka dla Hodowli i Nasiennictwa Roślin Uprawnych. Streszczenia prac. Konferencja Nauka dla Hodowli i Nasiennictwa Roślin Uprawnych. Zakopane 2001.
Google Scholar
Czembor H. J., Wiewióra M. 2001. Dziedziczenie tolerancji na toksyczne działanie glinu u wybranych odmian pszenicy jarej (Triticum aestivum L.). 220: 45 — 52.
Google Scholar
Flor H. H. 1955. Host-parasite interaction in flax rust-its genetics and other implications. Phytopathology 45: 680 — 685.
Google Scholar
Gnanesh B. N., Mitchell Fetch J., Menzies J. G., Beattie A. D., Eckstein P. E., McCartney C. A. 2013. Chromosome location and allele-specific PCR markers for marker-assisted selection of the oat crown rust resistance gene Pc91. Mol. Breeding 32: 679 — 686.
Google Scholar
Goutam U., Kukreja S., Yadav R., Salaria N., Thakur K., Goyal A. K. 2015. Recent trends and perspectives of molecular markers against fungal diseases in wheat. Front Microbiol. 6: 861.
Google Scholar
Górny A. G. 2004. Zarys genetyki zbóż. Tom 1. Jęczmień, pszenica i żyto. Wyd. Instytut Genetyki Roślin PAN, Poznań: 181 — 327.
Google Scholar
Hao Y., Liu, A., Wang Y., Feng D., Gao J., Li X., Liu S., Wang H. 2008. Pm23: a new allele of Pm4 located on chromosome 2AL in wheat. Theor. Appl. Genet. 117 (8): 1205 — 1212.
Google Scholar
Helguera M., Khan I. A., Dubcovsky J. 2000. Development of PCR markers for the wheat leaf rust resistance gene Lr47. Theor. Appl. Genet. 100: 1137 — 1143.
Google Scholar
Hiebert C. W., Thomas J. B., McCallum B .D., Somers D. J. 2008. Genetic mapping of the wheat leaf rust resistance gene Lr60 (LrW2). Crop Sci. 48: 1020 — 1026.
Google Scholar
Hovhannisyan N. A., Dulloo M. E., Yesayan A. H., Knupffer H., Amari A. 2011. Tracking of powdery mildew and leaf rust resistance genes in Triticum boeticum and T. urartu, wild relatives of common wheat. Czech J. Plant Breed. 47 (2): 45 — 57.
Google Scholar
Hsam S. L. K., Lapochkina I. F., Zeller F. J. 2003. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em. Thell) 8. Gene Pm32 in a wheat Aegilops speltoides translocation line. Euphytica 133: 367 — 370.
Google Scholar
Huang X. Q., Röder M. S. 2004. Molecular mapping of powdery mildew resistance genes in wheat. Euphytica 137: 203 — 223.
Google Scholar
Huang L., Brooks S. A., Li W., Fellers J. P., Trick H. N., Gill B. S. 2003 a. Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploidy genome of bread wheat. Genetics 164: 655 — 664.
Google Scholar
Huang L., Gill B. S. 2001. An RGA — like marker detected all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat. Theor. Appl. Genet. 103: 1007 — 1013.
Google Scholar
Huang X. Q., Hsam S. L. K., Zeller F. J., Wenzel G., Mohler V. 2000. Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor. Appl. Genet. 101: 407 — 414.
Google Scholar
Huang X. Q., Wang L. X., Xu M. X., Röder M. S. 2003 b. Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor. Appl. Genet. 106: 858 — 865.
Google Scholar
Huerta-Espino J., Singh R. P., Pérez-López J. B. 2009. Phenotypic Variation Among Leaf Rust Isolates From Durum Wheat In Northwestern Mexico. 12th International Cereal Rust and Powdery Mildews Conference, October 13–16, Antalya — Turcja. Abstract Book, pp. 29.
Google Scholar
Hysing S. C., Merker A., Liljeroth E., Koebner R. M. D., Zeller F. J., Hsam S. L. K. 2007. Powdery mildew resistance in 155 Nordic bread wheat cultivars and landraces. Hereditas 144: 102 — 119.
Google Scholar
Hysing S. H., Singh R. P., Huerta-Espino J., Merker A., Liljeroth E., Diaz O. 2006. Leaf rust (Puccinia triticina) resistance in wheat (Triticum aestivum) cultivars grown in Northern Europe 1992–2002. Hereditas 143: 1 — 14.
Google Scholar
Jańczak C., Pawlak A. 2006. Występowanie i szkodliwość mącznika prawdziwego (Blumeria graminis) w pszenicy ozimej w latach 2003–2005. Postępy w Ochronie Roślin 46 (2): 538 — 542.
Google Scholar
Ji J., Qin B., Wang H., Cao A., Wang S., Chen P., Zhuang L., Du Y., Liu D., Wang X. 2008. STS markers for powdery mildew resistance gene Pm6 in wheat. Euphytica 163: 159 — 165.
Google Scholar
Kimber G., Feldman M. 1987. Wild wheat: an introduction. Department of Agronomy University of Missouri-Columbia, Columbia, Missouri, USA. Plant Genetics The Weizmann Institute of Science Rehovot, Israel.
Google Scholar
Kochman J., Węgorek W. 1997. Ochrona Roślin. Choroby infekcyjne: Wyd. V, Plantpress, Kraków: 445 — 447.
Google Scholar
Kolmer J. A. 1996. Genetics of resistance to wheat leaf rust. Annu. Rev. Phytopathol. 34: 435 — 455.
Google Scholar
Kowalczyk K., Hsam S. L. K, Zeller F. J. 1998. Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em. Thell.). XI. Cultivars grown in Poland. J. Appl. Genet. 39 (3): 225 — 236.
Google Scholar
Larson, S., Kadyrzhanova, D., McDonald, C., Sorrells M., Blake, T. K. 1996. Evaluation of barley Chromosome 3 yield QTL in a- backcross F2 Population using PCR-STS markers. Theor. Appl. Genet. 93: 618 — 625.
Google Scholar
Leśniowska-Nowak J., Grądzielewska A., Majek M. 2013. Identyfikacja genów odporności na rdzę brunatną w wybranych europejskich odmianach pszenicy zwyczajnej oraz opracowanie warunków Multiplex PCR. Annales Universitatis Mariae Curie-Skłodowska Lublin - Polonia Vol. LXVIII (3) SECTIO E: 20 — 28.
Google Scholar
Lillemo M., Asalf B., Singh R.P., Huerta-Espino J., Chen X. M., He Z. H., Bjørnstad A. 2008. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor. Appl. Genet. 116: 1155 — 1166.
Google Scholar
Liu Z., Sun Q., Ni Z., Yang T. 1999. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat. Plant Breed. 118: 215 — 219.
Google Scholar
Liu J., Liu D., Tao W., Li W., Wang S., Chen P., Cheng S., Gao D. 2000. Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed. 119: 21 — 24.
Google Scholar
Liu Z., Sun Q., Ni Z., Nevo E., Yang T. 2002. Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123: 21 — 29.
Google Scholar
Lopez-Pardo R., Barandalla L., Ritter E., de Galarreta J. I. R. 2013. Validation of molecular markers for pathogen resistance in potato. Plant Breeding 132: 246 — 251.
Google Scholar
Ma Z.Q., Wei J.B., Cheng S.H. 2004. PCR-based markers for the powdery mildew resistance gene Pm4a in wheat. Theor. Appl. Genet. 109: 140 — 145.
Google Scholar
Malepszy S. 2001. Biotechnologia Roślin. Wydawnictwo Naukowe PAN, Warszawa.
Google Scholar
McIntosh R. A., Hart G. E., Devos K. M., Gale M. D., Rogers W. J. 1998. Catalogue of gene symbols for wheat. In: Slinkard A. E. (ed.). Proc. 9th Int. Wheat Genet Symp. 5: 13 — 72. Univ. Extension Press. University of Saskatchewan Saskatoon.
Google Scholar
McIntosh R. A., Wellings C. R., Park R. F. 1995. Wheat Rust: an atlas of resistance genes. CSIRO, Australia, Kluwer Academic Publishers, Dordrecht, The Netherlands.
Google Scholar
Mesterházy Á., Bartoš P., Goyeau H. 2000. European virulence survey for leaf rust in wheat. Agronomie 20: 793 — 804.
Google Scholar
Miranda L. M., Murphy J. P., Leath S., Marshall D. 2006. Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor. Appl. Genet. 113: 1497 — 1504.
Google Scholar
Miranda L. M., Murphy J. P., Marshall D., Cowger C., Leath S. 2007. Pm35: a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor. Appl. Genet. 114: 1451 — 1456.
Google Scholar
Mohler V., Hsam S. L. K., Zeller F. J., Wenzel, G. 2001. An STS marker distinguishing the rye-derived poldery mildew resistance alleles At the Pm8/Pm17 locus of common Wheat. Plant Breed. 120: 448 — 450.
Google Scholar
Nematollahi G., Mohler V., Wenzel G., Zeller F. J., Hsam S. L. K. 2008. Microsatellite mapping of powdery mildew resistance allele Pm5d from common wheat line IGV1-455. Euphytica 159: 307 — 313.
Google Scholar
Neu C., Stein N., Keller B. 2002. Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45: 737 — 744.
Google Scholar
Perugini L. D., Murphy J. P., Marshall D., Brown-Guedira G. 2008. Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii. Theor. Appl. Genet. 116: 417 — 425.
Google Scholar
Pietrusińska A., Czembor J. H., Czembor P. Cz. 2011. Pyramiding of two resistance genes for leaf rust and powdery mildew resistance in common wheat. Cereal Research Comm. 39 (4): 577 — 588.
Google Scholar
Pietrusińska A., Czembor J. H. 2014. Struktura wirulencji populacji Blumeria graminis f. sp. tritici występującej na terenie Polski w latach 2012–2013. Biul. IHAR 274: 15 — 25.
Google Scholar
Pietrusińska A., Czembor P. Cz., Czembor J. H. 2013. Lr39 + Pm21, as a new effective combination of resistance genes for leaf rust and powdery mildew. Czech J. Genet. Plant Breed. 49: 109 — 115.
Google Scholar
Pietrusińska 2010. Wykorzystanie markerów molekularnych do wprowadzania genów odporności na rdzę brunatną (Puccinia recondita f. sp. tritici) i mączniaka prawdziwego (Blumeria graminis f. sp. tritici) do pszenicy ozimej. Biul. IHAR 256: 31 — 54.
Google Scholar
Prabhu K. V., Singh A. K., Basavaraj S. H., Cherukuri D. P., Charpe A., Gopala Krishnan S., Gupta S. K., Joseph M., Koul S., Mohapatra T., Pallavi J. K., Samsampour D., Singh A., Singh V. K., Singh A., Singh V. P. 2009. Marker assisted selection for biotic stress resistance in wheat and rice. Indian J. Genet 69 (4): 305 — 314.
Google Scholar
Robert O., Abelard C., Dedryve F. 1999. Identification of molecular markers for the detection of the yellow rust resistance genes Yr17 in wheat. Mol. Breed. 5:167 — 175.
Google Scholar
Saini R.G., Kaur M., Singh B., Sharma S., Nanda G. S., Nayar S. K. 2002. Lr48 and Lr49, noval hypersensitive adult plant leaf rust resistance genes in wheat (Triticum aestivum L.). Euphytica 124: 365 — 370.
Google Scholar
Seyfarth, R., Feuillet, C., Schachermayr, G., Messmer, M., Winzeler, M., Keller, B., 2000. Molecular mapping of the adult-plant leaf rust resistance gene Lr13 in wheat (Triticum aestivum L.). J. Genet. & Breed. 54: 193 — 198.
Google Scholar
Singh S., Sidhu J. S., Huang N., Vikal Y., Li Z., Brar D. S., Dhaliwal H. S., Khush G. S. 2001. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor. Appl. Genet. 102: 1011 — 1015.
Google Scholar
Singh R. P., McIntosh R. A. 1984. Complementary genes for resistance to Puccinia recondita tritici in Triticum aestivum II. Cytogenetic studies. Can. J. Genet. Cytol. 26: 736 — 742.
Google Scholar
Singh S., Franks C. D., Huang L., Brown-Guedira, G. L., Marshall D. S., Gill B.S. 2004. Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theor. Appl. Genet. 108: 586 — 591.
Google Scholar
Singrün C., Hsam, S. L. K., Hartl L., Zeller F. J., Mohler V. 2003. Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em, Thell.). Theor. Appl. Genet. 106: 1420 — 1424.
Google Scholar
Song W., Xie H., Liu Q., Xie C.J., Ni Z.F., Yang T. M., Sun Q., Liu Z. Y. 2007. Molecular identification of Pm12-carrying introgression line in wheat using genomic and EST-SSR markers. Euphytica 158: 95 — 102.
Google Scholar
Spielmeyer W., McIntosh, R. A., Kolmer J. 2005. Powdery mildew resistance and Lr34/Yr18 to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor. Appl. Genet. 111: 731 — 735.
Google Scholar
Stępień Ł., Golka L., Chełkowski J. 2003. Lear rust resistance genes of wheat: identification in cultivars and resistance sources. J. Appl. Genet. 44 (2): 139 — 149.
Google Scholar
Suenaga K., Singh R. P., Huberta-Espino J., William H. M. 2003. Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for rust and stripe rust resistance in bread wheat. Phytopathology 93: 881 — 890.
Google Scholar
Tommasini L., Yahiaoui N., Srichumpa P., Keller B. 2006. Development of functional markers specific for seven Pm3 resistance alleles and their validation in the bread wheat gene pool. Theor. Appl. Genet 114: 165 — 175.
Google Scholar
Tratwal A., Jakubowska M. 2004. Ocena przydatności systemów wspomagania decyzji o ochronie pszenicy ozimej przed mączniakiem prawdziwym na terenie Wielkopolski. Postępy w Ochronie Roślin 44: 1169 — 1172.
Google Scholar
Tyrka M., Chełkowski J. 2003. Enhancing the resistance of triticale by using genes from wheat and rye. J. Appl. Genet. 45 (3): 283 — 295.
Google Scholar
Vanzetti L. S., Campos P., Demichelis M., Lombardo L. A., Aurelia P. R., Vaschetto L. M., Bainotti C. T., Helguera M. 2011. Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers. Electronic Journal of Biotechnology ISSN: 0717-3458: 1 — 14.
Google Scholar
Vida G., Gál M., Uhrin A., Veisz O., Syed N. H., Flavell A. J., Wang Z., Bedő Z. 2009. Molecular markers for the identification of resistance genes and marker-assisted selection in breeding wheat for leaf rust resistance. Euphytica 170: 67 — 76.
Google Scholar
William M., Langridge P., Trethowan R., Dreisigacker S., Crouch J. 2008. Genomics of wheat, the basis of our daily bread. Genomics of Tropical Plants 15: 515 — 548.
Google Scholar
William H. M., Crosby M., Trethowan R., Ginkel M., Mujeeb-Kazi A., Pfeiffer W., Khairallah M., Hoisington D. 2003. Molecular marker service laboratory at CIMMYT: An interface between the laboratory and the field. 10th Intern. Wheat Genet. Symp., Paestum, Italy 2: 852 — 854.
Google Scholar
Wiśniewska H., Błaszczyk L., Chełkowski J. 2007. Charakterystyka genotypów pszenicy pod kątem odporności na fuzariozę kłosów, mączniaka prawdziwego i rdzę brunatną. Postępy Nauk Rolniczych 6: 75 — 88.
Google Scholar
Witkowska K., Śmiałowski T., Witkowski E. 2011. Zależność plonu rodów pszenicy ozimej od stopnia porażenia przez Stagonospora nodorum i Puccinia triticina w zróżnicowanych warunkach polowych. Biul. IHAR 262: 47 — 58.
Google Scholar
Woźniak-Strzembicka A. 2003. Wirulencja populacji Puccinia recondita f. sp. tritici w Polsce w latach 1998–2001. Biul. IHAR 230: 109 — 117.
Google Scholar
Xie C., Sun Q., Ni Z., Yang T., Nevo E., Fahima T. 2003. Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor. Appl. Genet. 106: 341 — 345.
Google Scholar
Yi Y. J., Liu H.Y., Haung X. Q., An L. Z., Wang F., Wang Z. L. 2008. Development of molecular markers linked to the wheat powdery mildew resistance gene Pm4b and marker validation for molecular breeding. Plant Breed. 127: 116 — 120.
Google Scholar
Zeller F. J., Lutz J., Reimlein E. I., Limpert E., Koenig J. 1993 a. Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L). II. French cultivars. Agronomie 13: 201 — 207.
Google Scholar
Zhu Z., Zhou R., Kong X., Dong Y., Jia J. 2005. Microsatellite markers linked to a 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat. Genome 48: 585 — 590.
Google Scholar
Authors
Aleksandra Pietrusińskaa.pietrusinska@ihar.edu.pl
Instytut i Hodowli i Aklimatyzacji Roślin, Radzików, Krajowe Centrum Roślinnych Zasobów Genowych, Pracownia Gromadzenia i Oceny Roślin Poland
Authors
Jerzy H. CzemborInstytut i Hodowli i Aklimatyzacji Roślin, Radzików, Krajowe Centrum Roślinnych Zasobów Genowych, Pracownia Gromadzenia i Oceny Roślin Poland
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