Evaluation of cannabinoid content in selected varieties of Cannabis sativa L.
Grażyna Mańkowska
sekretariat@iwnirz.plInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Aleksandra Luwańska
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Karolina Wielgus
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Jan Bocianowski
Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
The study rated the cannabinoid content in hemp varieties of Cannabis sativa L. that have been obtained from Gene Bank of Institute of Natural Fibres and Medicinal Plants in Poznań. The experiment with hemp was conducted in 2014 at the Experimental Farm in Pętkowo (Wielkopolska Region). After collecting the plant material analysis of the content of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) was performed in the six varieties of hemp of European and Asian origins. For these parameters correlation coefficients were calculated and analysis of variance was performed using the software STATISTICA 12. The results showed significant differences in the contents of THC and CBD, and the relationship between the two cannabinoid contents in the hemp varieties.
Supporting Agencies
Keywords:
cannabidiol, varieties of hemp, tetrahydrocannabinolReferences
Białousowa J., Bartosik A., Kurhański M., Nagórski A., Tumalewicz B. 1958. Konopie, rośliny włókniste (Fibrous Plants). PWRil, Warszawa: 193 — 323.
Google Scholar
Bosca I., Karus M. 1997. Der Hanfanbau. Heidelberg, C. F. Muller: 195.
Google Scholar
De Backer. B., Debrus B., Lebrun, P., Theunis L., Dubois N., Decock L., Verstraete A., Hubert P., Charlier C. 2009. Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative of major cannabinoids in cannabis plant material. J. Chromatograf, B: Anal. Tech Biomed. Life Sci. 877: 4115 — 4124.
Google Scholar
Galal A. M., Slade D., Gul W., El-Alfy A. T., Ferreira D., El Sohly M. A. 2009. Naturally occurring and related synthetic cannabinoids and their potential therapeutic applications. Recent Patents on CNS Drug Discovery 4: 112 — 36.
Google Scholar
Grabowska L., Mańkowska G., Baraniecki P. 2004. Zasoby genowe Cannabis sativa L. w Instytucie Włókien Naturalnych w Poznaniu 497: 53 — 57.
Google Scholar
Jądrzak R., Biskupski M. 1999. Zastosowanie wysoko sprawnej chromatografii cieczowej (HPLC) w analizie kannabinoli, Problemy Kryminalistyki 224: 5 — 10.
Google Scholar
Kojoma M., Seki H., Yoshida S., Muranaka T. 2006. DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in „drug-type” and „fibre-type” Cannabis sativa L. Forensic Sci. Int. 159: 132 — 140.
Google Scholar
Lewis R., Ward S., Johnson R., Burns D. T. 2005. Distribution of the principal cannabinoids within bars of compressed cannabis resin. Anal. Chim. Acta 538: 399 — 405.
Google Scholar
Mackie K. 2007. From active ingredients to the discovery of the targets: the cannabinoid receptors. Chern. Biodivers. 4: 1693 — 706.
Google Scholar
Mańkowska G., Strybe M., Chudy M., Luwańska A., Baraniecki P. 2010. Ocena cech użytkowych wybranych odmian konopi Cannabis sativa L. zgromadzonych w Instytucie Włókien Naturalnych i Roślin Zielarskich w 2008 roku. Zeszyty Problemowe Postępów Nauk Rolniczych 555: 529 — 536.
Google Scholar
Mechoulam R., Hanus L. 2013. Inne rodzaje kannabinoidów. [In:] Marihuana i obłęd. Castle D, Murray R M, D’ Souza D C (red.), MediPage. Warszawa: 22 — 28.
Google Scholar
Rymanowski M. 2014. Konopie, przegląd zagadnień związanych z oznaczaniem sumarycznej zawartości delta-9-tetrahydrokannabinolu (D-9-THC) oraz kwasu delta-9-tetrahydrokannabinolowego (D-9-THCA-A) Problemy Kryminalistyki 285 (3): 1 — 22.
Google Scholar
Scheifele G., Dragla P. 2000. 1999. report on environment and latitude effect on THC levels of industrial hemp varieties grown in Ontario. Ontario Ministry of Agriculture, Food and Rural Affairs.
Google Scholar
Scheifele G., Hinz H., Davies K., Calder K-J., Bowman M., Guillemette L. 1999. 1998 Ontario studies in determining the genetic stability, environment and latitude effect on the levels of delta -9 THC for industrial hemp varieties. Ontario Ministry of Agriculture, Food and Rural Affairs.
Google Scholar
Szukalski B. 2005. Kannabinoidy. W: Kompendium wiedzy o środkach uzależniających. Wydawnictwo Instytutu Psychiatrii i Neurologii, Warszawa, 4: 5 — 15 .
Google Scholar
Tkaczyk M., Florek E., Piekoszewski W. 2012. Marihuana i kannabinoidy jako leki. Przegl. Lek. 10: 1095 — 1097.
Google Scholar
Wielgus K., Przewoźna J., Mańkowska G., Grabowska L., Podralska M., Słomski R. 2010. Polimorfizm genu syntazy THCA w zasobach genowych konopi siewnych — Cannabis sativa L. zgromadzonych w Instytucie Włókien Naturalnych i Roślin Zielarskich. Zeszyty Problemowe Postępów Nauk Rolniczych, 555: 457 — 464.
Google Scholar
Authors
Grażyna Mańkowskasekretariat@iwnirz.pl
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Aleksandra LuwańskaInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Karolina WielgusInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Jan BocianowskiUniwersytet Przyrodniczy w Poznaniu Poland
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