Identification of SSR markers useful for construction of genetic map and mapping of angular leaf spot resistance genes in cucumber (Cucumis sativus L.)
Renata Słomnicka
skrzynka_ib-kghbr@sggw.edu.plKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Adrianna Pietluch
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Justyna Łęczycka
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Anna Doraczyńska
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Aleksandra Korzeniewska
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Helena Olczak-Woltman
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Katarzyna Niemirowicz-Szczytt
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Grzegorz Bartoszewski
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Abstract
The aim of this study was identification of SSR markers useful for construction of genetic map of cucumber population that consists of 110 recombinant inbred lines (RILs). RILs population segregating for angular leaf spot (ALS) resistance was developed by crossing two inbred lines, an American line Gy14 showing tolerance for angular leaf spot and a Polish line B10, susceptible to this disease. A set of SSR markers was preliminary examined for polymorphism using bioinformatic analysis. There were 222 selected SSR markers and 160 of them were further tested on parental lines and chosen RILs. The polymorphism was confirmed for 103 (64.4%) SSR markers. Fifty two SSR markers were found to be useful for construction of genetic map of cucumber population Gy14 × B10 and further mapping of angular leaf spot resistance genes. Preliminary results suggest that SSR00398 located on chromosome 5 may be linked to angular leaf spot resistance gene.
Supporting Agencies
Keywords:
Cucumis sativus, angular leaf spot, ALS, SSR markers, Pseudomonas syringae pv. lachrymansReferences
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Authors
Renata Słomnickaskrzynka_ib-kghbr@sggw.edu.pl
Katedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Adrianna PietluchKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Justyna ŁęczyckaKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Anna DoraczyńskaKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Aleksandra KorzeniewskaKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Helena Olczak-WoltmanKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Katarzyna Niemirowicz-SzczyttKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
Authors
Grzegorz BartoszewskiKatedra Genetyki Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
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Copyright (c) 2015 Renata Słomnicka, Adrianna Pietluch, Justyna Łęczycka, Anna Doraczyńska, Aleksandra Korzeniewska, Helena Olczak-Woltman, Katarzyna Niemirowicz-Szczytt, Grzegorz Bartoszewski
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