Obtaining of haploids with use of Hordeum bulbosum method and mutagenesis and analysis of quantitative traits variation in spring barley DH lines
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Tadeusz Adamski
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Maria Surma
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Jan Bocianowski
Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
In performed studies on the use of mutagenesis in DH system, the seeds of malting barley cultivar Rudzik were treated with laser light and chemomutagen — N-methyl-N-nitroso urea (MNU). The plants grown from the treated plants were crossed with Hordeum bulbosum. The effectiveness of haploid production was measured by proportion of the obtained seeds to the number of pollinated florets and number of haploids to number of pollinated florets and immature embryos transferred to in vitro culture. The obtained results as compared to control (non-treated seeds) indicates that the applied dose of MNU decreased the effectiveness of haploid production. In result of diploidization, a numerous DH plants were obtained which were propagated and used for estimation of quantitative traits variability in a field trial. The use of multivariate statistical method indicates that DH lines were characterized by a broader range of traits variability in comparison with their initial cultivar Rudzik.
Keywords:
dihaploid lines, Hordeum bulbosum, mutagen, spring barley, quantitative traitsReferences
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Authors
Tadeusz AdamskiInstytut Genetyki Roślin PAN w Poznaniu Poland
Authors
Maria SurmaInstytut Genetyki Roślin PAN w Poznaniu Poland
Authors
Jan BocianowskiUniwersytet Przyrodniczy w Poznaniu Poland
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