Genetic similarity among cultivars of winter barley (Hordeum vulgare L.) revealed by random amplified polymorphic DNA (RAPD)
Anetta Kuczyńska
office@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Jan Bocianowski
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Piotr Masojć
Akademia Rolnicza, Szczecin (Poland)
Maria Surma
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Tadeusz Adamski
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Abstract
The aim of this study was to detect genetic similarity among cultivars of winter barley using RAPD (random amplified polymorphic DNA) method. RAPD polymorphism was studied in eight winter barley cultivars: Marinka, Gil, Tramp, Kroton, Tiffany, Horus, Kos and Sigra. Twenty-four 10-mer primers were tested in each cultivar. 172 amplification products were observed, from which 115 were polymorphic. Genetic similarity was estimated according to the formula given by Nei and then these results were used for the hierarchical grouping of cultivars. Results of hierarchical grouping were presented on the dendrogram. The highest genetic similarity was revealed between the cultivars Tiffany and Horus, and Marinka and Kos, whereas the lowest genetic similarity was found for Kroton and Tiffany, and Kroton and Sigra.
Keywords:
barley, genetic similarity, hierarchical grouping, RAPDReferences
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Authors
Anetta Kuczyńskaoffice@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Jan BocianowskiInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Piotr MasojćAkademia Rolnicza, Szczecin Poland
Authors
Maria SurmaInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Tadeusz AdamskiInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
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Copyright (c) 2003 Anetta Kuczyńska, Jan Bocianowski, Piotr Masojć, Maria Surma, Tadeusz Adamski
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