Separation of the fractions of potato juice of different molecular weight and characterization of their biological activities
Grażyna Lewandowicz
grazyna.lewandowicz@up.poznan.plKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Przemysław Kowalczewski
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Wojciech Białas
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Anna Olejnik
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Joanna Rychlik
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
The clinical trials on the medicinal use of potato juice proved its efficiency in the therapy of gastrointestinal disorders. However, the literature data don’t determine which substances actually reveal the therapeutic effect. The aim of the work was estimation of the biological activity of potato juice sprayed in the spray dryer, as well as its fractions of different molecular mass. Fresh potato juice was spray dried or alternatively, cryoconcentrated. As the result of the latter process fivefold concentrated potato juice has been obtained. This product was separated by means of gel permeation chromatography. The isolated fractions were analysed in terms of their capability to inhibit the proliferation and to reduce viability of human cancer cell lines. Human normal cell lines were used as a reference. Our studies proved that processed potato juice, similar to the fresh one, reveals cytotoxicity potential to stomach and colon cancer cells. The spray drying only slightly decreased biological activity of potato juice. It is possible to obtain fivefold concentrated potato by application of cryoconcentration process. That product could be effectively separated by means gel filtration process. The highest cytotoxicity, specifically targeted to the cancer cells, was revealed in non-protein, thermally stable fraction of molecular mass below 1 kDa.
Supporting Agencies
Keywords:
cytotoxicity, cryoconcentration, potato juice, separation, spray dryingReferences
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Authors
Grażyna Lewandowiczgrazyna.lewandowicz@up.poznan.pl
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Przemysław KowalczewskiKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Wojciech BiałasKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Anna OlejnikKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Joanna RychlikKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
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