Identification of puroindoline alleles in Polish wheat cultivars by molecular markers
Monika Langner
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Bolesław Salmanowicz
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Abstract
Endosperm hardness plays an important role in quality characteristics of cultivated wheat (Triticum aestivum L.) and has a significant effect on milling and baking processes and on the quality of the final product. Gene sequence variation and mutations to the two puroindoline genes (Pina-D1 and Pinb-D1), located at the Ha locus on the chromosome 5D, account for the majority of variation in wheat kernel texture. The objective of the presented study was to characterize the allelic forms of puroindoline genes in Polish wheat cultivars. For a total of 69 wheat cultivars the measurement of SKCS grain hardness was performed, and puroindoline alleles were identified by molecular markers. On the basis of mean SKCS hardness eight cultivars were classified as a hard type, 60 cultivars as a mixed one and one as a soft type. In all the cultivars the Pina-D1a allele was identified. To identify alleles of Pinb-D1 gene, PCR product of this gene was digested to small fragments by BsrBI restriction enzyme. Among the grain samples, 35 cultivars were found to possess the wild-type Pinb-D1a allele.
Keywords:
PCR, allelic variation, kernel hardness, puroindolines, restriction enzyme, wheatReferences
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Authors
Bolesław SalmanowiczInstytut Genetyki Roślin PAN w Poznaniu Poland
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Copyright (c) 2009 Monika Langner, Bolesław Salmanowicz
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